The Botanic, physicochemical, phenolic and antioxidant properties of Muş Basin honeys

Yıl 2025, Cilt: 8 Sayı: 2, 175 - 192, 31.12.2025

Sevgi Kolaylı , Erol Tunca , Aslı Özkök

https://doi.org/10.35206/jan.1689764

Öz

: Muş is a prominent honey-producing area located in the central part of the Eastern Anatolia region, bordered by eight different provinces. In this study, the characteristic properties of honey from the Muş region, selected as the pilot area, were examined. The characteristics of the honey were determined based on its botanical origin, physicochemical properties, phenolic compound profile, and antioxidant potential. The honeys are monofloral or heterofloral blossom honeys, rich in Astragalus spp. and clover (Trifolium spp.) flowers, with smaller contributions from Lotus spp., Liliaceae, Rosaceae, Plantago spp., Poaceae, Elymus spp., Sanguisorba spp., Berberidaceae, Centaurea spp., Cistaceae, Rumex spp., Papaveraceae, and Asteraceae flowers. According to the Hunter Lab color scale, the honeys were found to have light, greenish, and yellowish tones. The average electrical conductivity (EC) was 0.34± 0.05mS/cm, indicating a very low moisture content of 15.33±0.75%. The honeys, which exhibited low HMF and high diastase activity, had an average proline content of 543±138 mg/kg. The ratio of fructose to glucose was found to be 1.23 in the honeys with low sucrose and maltose content. The average of total phenolic content (TFC) was to be 20.29± 2.90 mg GAE/100g, with notable antioxidant activity. The honeys were rich in gallic acid, chrysin, p-hydroxybenzoic acid, ferulic acid, and quercetin. These analyses demonstrate that the Muş region honeys possess rich floral characteristics especially, Astragalus spp. and Trifolium spp, typical of light-colored blossom honeys in the Eastern Anatolia region. This study provides valuable data for elucidating the geographic characteristics of Muş region honeys.

Anahtar Kelimeler

Honey , antioxidant , Astragalus spp , Trifolium spp , East Anatolia

The Botanic, physicochemical, phenolic and antioxidant properties of Muş Basin honeys

Öz

Muş is a prominent honey-producing area located in the central part of the Eastern Anatolia region, bordered by eight different provinces. In this study, the characteristic properties of honey from the Muş region, selected as the pilot area, were examined. The characteristics of the honey were determined based on its botanical origin, physicochemical properties, phenolic compound profile, and antioxidant potential. The honeys are monofloral or heterofloral blossom honeys, rich in Astragalus spp. and clover (Trifolium spp.) flowers, with smaller contributions from Lotus spp., Liliaceae, Rosaceae, Plantago spp., Poaceae, Elymus spp., Sanguisorba spp., Berberidaceae, Centaurea spp., Cistaceae, Rumex spp., Papaveraceae, and Asteraceae flowers. According to the Hunter Lab color scale, the honeys were found to have light, greenish, and yellowish tones. The average electrical conductivity (EC) was 0.34± 0.05mS/cm, indicating a very low moisture content of 15.33±0.75%. The honeys, which exhibited low HMF and high diastase activity, had an average proline content of 543±138 mg/kg. The ratio of fructose to glucose was found to be 1.23 in the honeys with low sucrose and maltose content. The average of total phenolic content (TFC) was to be 20.29± 2.90 mg GAE/100g, with notable antioxidant activity. The honeys were rich in gallic acid, chrysin, p-hydroxybenzoic acid, ferulic acid, and quercetin. These analyses demonstrate that the Muş region honeys possess rich floral characteristics especially, Astragalus spp. and Trifolium spp, typical of light-colored blossom honeys in the Eastern Anatolia region. This study provides valuable data for elucidating the geographic characteristics of Muş region honeys.

Anahtar Kelimeler

Honey , antioxidant , Astragalus spp , Trifolium spp , East Anatolia , phenolic

Kaynakça

• Abbasi, H. (2020). The effect of type (Astragalus, Ziziphus, Citrus and Thyme), and storage conditions on the most important qualitative characteristic of honey. Iranian Food Science & Technology Research Journal, 16(1), 85–101.
• Abou-Shaara, H. F., Owayss, A. A., Ibrahim, Y. Y., & Basuny, N. K. (2017). A review of impacts of temperature and relative humidity on various activities of honeybees. Insectes Sociaux, 64(4), 455–463.
• AOAC. (1998). In P. Cunniff (Ed.), Official methods of analysis (16th ed.). Association of Official Analytical Chemists.
• Atasagun, B., Aksoy, A., & Martin, E. (2018). Morphological, anatomical, palynological, karyological and ecological remarks of Astragalus argaeus (Fabaceae) endemic to Turkey. Phytotaxa, 379(1), 118–130.
• Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70–76. https://doi.org/10.1006/abio.1996.0292
• Bogdanov, S., Lüllmann, C., Martin, P., Von Der Ohe, W., Russmann, H., et al. (1999). Honey quality and international regulatory standards: Review by the International Honey Commission. Bee World, 80(2), 61–69.
• Boutrou, I., Tsadila, C., Amoroso, C., & Mosialos, D. (2024). Antifungal activity exerted by Greek honeys and bacteria isolated from them. Journal of Apitherapy and Nature, 7(2), 170–189.
• Can, Z., Yildiz, O., Sahin, H., Turumtay, E. A., Silici, S., et al. (2015). An investigation of Turkish honeys: Their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chemistry, 180, 133–141.
• Çetinkaya, T., & Bayil-Oguzkan, S. (2024). Physicochemical characteristics of Astragalus honey obtained from Erzurum province. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 28, 185–190.
• Drivelos, S. A., Danezis, G. P., Halagarda, M., Popek, S., & Georgiou, C. A. (2021). Geographical origin and botanical type honey authentication through elemental metabolomics via chemometrics. Food Chemistry, 338, 127936.
• Erdemli, S., Colak, E., & Kendir, H. (2007). Determination of some plant and agricultural characteristics in Persian clover (Trifolium resupinatum L.). Journal of Agricultural Sciences, 13(3), 240–246.
• Ertekin, İ. (2021). Comparison of chemical composition and nutritive values of some clover species. International Journal of Chemistry and Technology, 5(2), 162–166.
• Escriche, I., Juan-Borrás, M., Visquert, M., & Valiente, J. M. (2023). An overview of the challenges when analysing pollen for monofloral honey classification. Food Control, 143, 109305.
• Fukumoto, L. R., & Mazza, G. (2000). Assessing antioxidant and prooxidant activities of phenolic compounds. Journal of Agricultural and Food Chemistry, 48(8), 3597–3604. https://doi.org/10.1021/jf000220w
• Gök, İ., & Tezçi, S. (2023). Determinants of geographical indicated Kırklareli oak honey consumption reasons with special reference to the influence of nutritional knowledge and health status. Food Health and Technology Innovations, 6(13), 515–539.
• Gül, E., & Dölarslan, M. (2021). Distribution and importance of some endemic Astragalus L. species in semi-arid environmentally sensitive areas: A case study from northern Turkey. Cerne, 27, e-102559.
• Güler, A., Bakan, A., Nisbet, C., & Yavuz, O. (2007). Determination of important biochemical properties of honey to discriminate pure and adulterated honey with sucrose (Saccharum officinarum L.) syrup. Food Chemistry, 105(3), 1119–1125.
• Hegazi, A. G., Al Guthami, F. M., Ramadan, M. F., Al Gethami, A. F., Craig, A. M., et al. (2022). Characterization of Sidr (Ziziphus spp.) honey from different geographical origins. Applied Sciences, 12(18), 9295.
• Kara Y, Can Z, Kolaylı S (2022). What should be the ideal solvent percentage and solvent-propolis ratio in the preparation of ethanolic propolis extract? Food Analytical Methods. 15(6):1707-1719.
• Kara, Y., & Birinci, C. (2024). Usability of the phenolic profile analysis method developed in RP-HPLC-PDA in natural products. Journal of Apitherapy and Nature, 7(1), 14–27. https://doi.org/10.35206/jan.1430767
• Kara, Y., Şahin, H., & Kolaylı, S. (2020). Geographical fingerprint of astragalus (Astragalus microcephalus Willd.) honey supplied from Erzincan region of Turkey. Uludağ Arıcılık Dergisi, 20(2), 123–131.
• Karlıdağ, S., & Kolaylı, S. (2025). Comparison of some mineral and heavy metal contents of blossom honey samples from Yamadağ and Battalgazi regions. Turkish Journal of Analytical Chemistry, 7(2), 182-190.
• Kemal, M., Esertaş, Z. Ü., Kanbur, E. D., Kara, Y., Özçelik, A. E., et al. (2023). Characterization of the black cumin (Nigella sativa L.) honey from Türkiye. Food Bioscience, 53, 102760.
• Khalil, M. I., & Sulaiman, S. A. (2010). The potential role of honey and its polyphenols in preventing heart disease: A review. African Journal of Traditional, Complementary and Alternative Medicines, 7(4).
• Kolaylı, S., & Birinci, C. (2024). A comparative study of solvent effect on propolis extraction by ultrasound-assisted extraction. Turkish Journal of Analytical Chemistry, 6(1), 11–17. https://doi.org/10.51435/turkjac.1445121
• Kolaylı, S., Can, Z., Yildiz, O., Şahin, H., & Karaoğlu, S. A. (2016). A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(sup3), 96–104.
• Kolaylı, S., Can, Z., Çakır, H. E., Okan, O. T., & Yildiz, O. (2018). An investigation on Trakya region oak (Quercus spp.) honeys of Turkey: Their physico-chemical, antioxidant and phenolic compounds properties. Turkish Journal of Biochemistry, 43(4), 362–374.
• Kurt, L., Ketenoğlu, O., Tuğ, G. N., & Şekerciler, F. (2015). Highland vegetation of Inner and Eastern Anatolia and the effects of global warming. In Climate Change Impacts on High-Altitude Ecosystems (pp. 275–288). Springer.
• Küçükaydın, S., Tel-Çayan, G., Çayan, F., Taş-Küçükaydın, M., & Eroğlu, B., et al. (2023). Characterization of Turkish Astragalus honeys according to their phenolic profiles and biological activities with a chemometric approach. Food Bioscience, 53, 102507.
• Louveaux J, Maurizio A, Vorwohl G (1978). Methods of melissopalynology. Bee World. 59(4):139-157. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG (2005). Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry. 91(3):571-577.
• Özkök, A. (2024). An important value of Turkey: Lavender (Lavandula stoechas L.) honey. Journal of Apitherapy and Nature, 7(1), 53–64.
• Scepankova, H., Combarros-Fuertes, P., Fresno, J. M., Tornadijo, M. E., Dias, M. S., et al. (2021). Role of honey in advanced wound care. Molecules, 26(16), 4784.
• Serrano, S., Rodríguez, I., Moreno, R., & Rincón, F. (2019). Detection of key factors affecting specific optical rotation determination in honey. CyTA - Journal of Food, 17(1), 574–580.
• Slinkard, K., & Singleton, V. L. (1977). Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1), 49–55. https://doi.org/10.1002/star.19780301107
• Singh, I., & Singh, S. (2018). Honey moisture reduction and its quality. Journal of food science and technology, 55(10), 3861-3871.
• Sultana, S., Foster, K., Lim, L. Y., Hammer, K., & Locher, C. (2022). A review of the phytochemistry and bioactivity of clover honeys (Trifolium spp.). Foods, 11(13), 1901.
• Tafere, D. A. (2021). Chemical composition and uses of honey: A review. Journal of Food Science and Nutrition Research, 4(3), 194–201.
• Turkish Food Codex. (2020). Türk Gıda Kodeksi - Bal Tebliği (No: 2020/7). Official Press.
• Uçurum, H. Ö., Tepe, Ş., Yeşil, E., Güney, F., Karakuş, S., et al. (2023). Characterization of Turkish pine honey according to their geographical origin based on physicochemical parameters and chemometrics. European Food Research and Technology, 249(5), 1317–1327.
• Ucurum, O., Tosunoglu, H., Takma, Ç., Birlik, P. M., & Berber, M., et al. (2024). Distinctive properties of the pine, oak, chestnut and multifloral blossom and honeydew honeys. European Food Research and Technology, 250(6), 1765–1774.
• Von Der Ohe, W., Oddo, L. P., Piana, M. L., Morlot, M., & Martin, P. (2004). Harmonized methods of melissopalynology. Apidologie, 35(Suppl. 1), 18–25. https://doi.org/10.1051/apido:2004050
• Young, G. W. Z., & Blundell, R. (2023). A review on the phytochemical composition and health applications of honey. Heliyon, 9(2), e13658.