Fatty Acid Analysis and Biological Activity of Jordanian Propolis

Yıl 2018, Cilt: 1 Sayı: 3 - 2nd Propolis Conference Special Issue, 33 - 33, 08.12.2018

Ashok K. Shakya Shankar Katekhaye Ghaleb A. Orıquat Rajashri R. Naık Rajashri R. Naık Anant Paradkar Hugo Fearnley James Fearnley

https://izlik.org/JA97AR35XP

Öz

Propolis is a resinous
natural product collected by bees (Apis
mellifera) from tree exudates which is widely used in folk medicine¹.
Reports on Jordanian Propolis reveal the presence of new chemical compound
4(Z)-1-3-dihydroxyeupha-7,24-dien-26-oic acid² along with other
compounds like pinobanksin-3-O-acetate,
pinocemberin, chrysin³ and lignoceric acid². The
present study was carried out to investigate the fatty acid composition,
antioxidant and xanthine oxidase inhibition activity of Jordanian Propolis,
collected from Al-Ghour region. The hexane extract of Jordanian Propolis
contains different fatty acids, which are reported first time, using GC-FID. The
major fatty acid identified were palmitic acid (44.5%), Oleic acid (18:1∆⁹cis, 24.6%), Arachidic acid (7.4%), Stearic acid (5.4%),
linoleic acid (18:2∆^9-12cis, 3.1%), caprylic acid
(2.9%),  lignoceric acid (2.6%), cis-11,14-eicosadienoic acid (20:2∆^11-14cis, 2.4%), palmitoleic
acid (1.5%), cis-11-eicosenoic acid (1.2%), α–linolenic acid (18:3∆^9-12-15cis, 1.1%), cis-13,16-docosadienoic acid (22:2∆^13-16cis, 1.0%), along with
minor constituents like saturated fatty acids. Antioxidant properties of the
hexane extract were determined via DPPH radical scavenging, β-carotene
bleaching assay and NO scavenging assay. The extract produced significant
antioxidant activity in-vitro. The
extract also exhibit appreciable xanthine oxidase inhibitory activity in-vitro.

Anahtar Kelimeler

Propolis , Naturel product , DPPH , In vitro

Kaynakça

• 1. Toreti, V.C., Sato, H.H., Pastore, G.M. and Park, Y.K., 2013. Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evidence-based complementary and alternative medicine, 2013. http://dx.doi.org/10.1155/2013/697390
• 2. Shaheen, S.A., Zarga, M.H.A., Nazer, I.K., Darwish, R.M. and Al-Jaber, H.I., 2011. Chemical constituents of Jordanian propolis. Natural product research, 25(14), 1312-1318.
• 3. Darwish, R.M., Ra'ed, J., Zarga, M.H.A. and Nazer, I.K., 2010. Antibacterial effect of Jordanian propolis and isolated flavonoids against human pathogenic bacteria. African Journal of Biotechnology, 9(36). 5966-5974