Spodoptera frugiperda Hücre Kültüründe Geliştirilen Autographa californica nükleer polihedrosis virüsünün (AcNPV) Replikasyonu Üzerine Türkiye Propolisinin Antiviral Etkisi

Yıl 2022, Cilt: 5 Sayı: 1, 69 - 81, 30.06.2022

Ömer Ertürk Ceren Başkan

https://doi.org/10.35206/jan.1053371

Öz

Bu çalışmada, Spodoptera frugiperda kültüründe geliştirilen Autographa californica nükleer polihedrozis virüsünün (AcNPV) replikasyonu üzerine Türkiye'den elde edilen propolis ekstraktlarının etkisi, sitopatik etkiler, hidroksil radikal aracılı DNA hasarı ve progeni virüs konsantrasyonundaki değişiklikler incelenerek araştırılmıştır. Propolis özü kullanarak sitopatik değişiklikler ve DNA hasarı üzerinde engelleyici etki bulduk. Propolis ekstraktlarının sitopatik değişiklikler ve hidroksil radikal aracılı DNA hasarı üzerinde kontrole benzer olduğu bulundu. Sf9 hücrelerinde emilim bölgesi üretimi açısından, propolis konsantrasyonu 3.125 ve 1.5625 µg/mL olduğunda kontamine kontrol hücrelerinde kontamine hücrelerin ortalama yüzdesi arasında önemli bir fark bulunmadı (sırasıyla 4.018 (plak oluşturan birim) (PFU) pfu/mL ve 5.145 pfu/mL) (p<0.05) ve kontamine kontrol hücrelerindeki ortalama emilim bölgesi sayısı ile propolis konsantrasyonundaki kontamine hücreler arasında anlamlı bir fark bulunmadı. 100, 50 μg/mL propolis ekstraktlarının konsantrasyonu 1 h pi dahil edildiğinde propolis ekstraktları ile virüs titresinde sırasıyla %98.70 ve %98.67 azalma bulundu. (p<0.05). Bununla birlikte, farklı propolis özütü konsantrasyonları, virüslerin sayısını önemli ölçüde azaltmıştır.

Anahtar Kelimeler

Baculovirus, Propolis, Antiviral aktivite, Virüs replikasyonu

Destekleyen Kurum

Yok

The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa californica nuclear polyhedrosis virus (AcNPV) Developed in Spodoptera frugiperda Cell Culture

Öz

In this study, the influence of propolis extracts gathered from Turkey on the replication of Autographa californica nuclear polyhedrosis virus (AcNPV) developed in Spodoptera frugiperda culture was researched through examining changes in cytopathic influences, hydroxyl radical mediated DNA damage and progeny virus concentration. By making use of propolis extract, we found inhibiting effect on cytopathic changes and DNA damage. It was found that propolis extracts on the cytopathic changes and hydroxyl radical-mediated DNA damage were similar to the control. The results showed that propolis has a protective effect on DNA exposed with H2O2. In terms of the production of occlusion bodies in Sf9 cells, no important difference was found between the average percentage of contaminated cells in contaminated control cells when the concentration of propolis is 3.125, and 1.5625 μg/mL (4.018 (Plaque forming units (PFU) pfu/mL and 5.145 pfu/mL respectively) (p<0.05) and no significant difference was found between the average number of occlusion bodies in the contaminated control cells and the contaminated cells in the concentration of propolis. Respectively 98.70% and 98.67% reduction were found in virus titter with propolis extracts when the concentration of propolis extracts 100, 50 μg/mL were included 1 h p.i. (p<0.05). However, different concentrations of propolis extract reduced the number of progeny viruses significantly.

Anahtar Kelimeler

Baculovirus, Propolis, Antiviral activity, Virus replication

Kaynakça

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