The Effects of Chicken GnRH on Serum Testosterone Concentration and Egg Fertility in Japanese Quail

Yıl 2018, Cilt: 1 Sayı: 1, 84 - 92, 24.12.2018

Hüseyin Baki Çiftçi Sedat Aktan Veli Can Başkar İskender Yıldırım Ali Aygün

Öz

The decrease in fertility of aged cocks results with decreased hatching rate. Male fertility is under the control of the hypothalamus-pituitary and testicular axis. Therefore, the objective of this study is to measure the effect of chicken GnRH-I (cGnRH-I) on serum testosterone concentration and egg fertility. Twenty weeks old male and female Japanese quail were kept in cages and fed ad libitum. Quails were randomly divided into 4 groups as phosphate buffered saline group (PBS), 5 µg cGnRH-I group, 20µg cGnRH-I group and group received no injection (Naturel group, N group). Each group included 8 replicates and each replicate made up randomly selected 8 females and 2 males. Only male birds were subcutaneously injected once a week for three weeks with 200 µl as PBS, 200 µl PBS containing 5 or 20µg cGnRH-I. One week after the last injection, eggs from each group were weekly collected and incubated for 11 days and broken on day 12 then the numbers of fertilized and unfertilized eggs were determined. Blood samples from the males were obtained from the jugular vein. Serum was extracted from the clotted blood by centrifuging. Total serum testosterone levels were measured by competitive immunochemistry. Injection of 5µg cGnRH increased serum testosterone concentration over PBS injected group other differences were not statistically different. Injection of cGnRH did not cause significant changes in fertility rates. To conclude, 20 weeks old male quails were injected with cGnRH-I and giving extra GnRH-I did not cause any difference in fertility, since there was plenty of GnRH in their blood.

Anahtar Kelimeler

GnRH, Fertility, Serum, Quail, Testosterone

Teşekkür

Thanks to Animal Research and Exploration Centre, Süleyman Demirel University, for providing the study material and environment.

Japon Bıldırcınlarında Tavuk GnRH'nin Serum Testesteron Konsantrasyonu ve Yumurtalarda Döllülük Üzerine Etkileri

Öz

Yaşlı erkeklerin fertilitesindeki düşüş çıkış oranında azalmayla sonuçlanır. Erkeğin fertilitesi hipotalamo-hipofisyal ve testis eksenin kontrolü altındadır. Böylece bu projenin amacı tavuk GnRH-I (cGnRH-I)’in serum testosteron konsantrasyonuna ve yumurta döllülük oranına etkisini ölçmektir. Yirmi haftalık erkek ve dişi Japon bıldırcınları kafeslere konuldu ve ad libitum beslendiler. Bıldırcınlar random olarak; fosfatla tamponlanmış tuz çözeltisi (PBS), 5 µg cGnRH-I, 20µg cGnRH-I enjektedilen grup ve enjeksiyon yapılmayan doğal grup (N) olmak 4 gruba ayrıldı. Her grup 8 alt grupa ayrıldı ve her bir alt gruba 8 dişi 2 erkek konuldu. Sadece erkekler haftada bir kez olmak üzere üç hafta boyunca 200 µl PBS, 5 veya 20 µg cGnRH-I içeren 200 µl PBS ile enjekte edildiler. Son enjeksiyondan bir hafta sonra yumurtalar haftalık toplandı, 11 gün boyunca kuluçka makinasında tutuldu ve 12. günde kırılarak döllü ve dölsüz yumurtalar belirlendi. Kan numuneleri erkelerin jugular damarından alındı. Serum; pıhtilaşan kan örneklerinden santrifüjle ayrıldı. Serumdaki toplam testesteron kompetetif ümmünokimya yöntemiyle ölçüldü. 5µg cGnRH enjeksiyonu serum testesteron konsantrasyonunu PBS enjekte dilen gruba kıyasla artırırken diğer faklar istatistiksel olarak önemli bulunmadı. GnRH enjeksiyonu fertilite oranında önemli değişmeye sebep olmadı. Bu çalışmada 20 haftalık genç bıldırcınlara cGnRH enjeksiyonu yapıldı. Hayvanlar genç oldukları için kanlarında yeteri kadar GnRH vardı ve fazladan GnRH enjekte etme bir farka sebep olmadı.

Anahtar Kelimeler

GnRH, Döllülük, Serum, Bıldırcın, Testosteron

Kaynakça

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