Akut Lenfoblastik Lösemili Çocuklarda Kemoterapinin Doğal Öldürücü NK Hücreler Üzerine Etkileri

Öz

Amaç: Kemoterapi almakta olan ve tedavisi kesilmiş akut lenfoblastik lösemili çocuklarda immünitenin önemli elemanı NK hücrelerinde kemoterapi ile değişiklikler oluşabilir. Yöntemler: Yaşları 2 ile 19 arasında değişen otuz üç ALL’li çocuk hasta immünfenotipleri, risk grupları ve klinik özellikleri kaydedilerek grup 1 ALL TRALL 2000 protokolüne göre protokol M’i bitirmiş , grup 2 idame kemoterapisi alan ve grup 3 tedavisi kesilmiş olarak sınıflandırıldı. Kontrol grubu grup 4 ise 2 ile 13 yaşları arasındaki on bir sağlıklı çocuktan oluşturuldu. Hasta ve kontrol grubundan bilgilendirilmiş onam ile alınan kan örneklerinde NK sitotoksisite deneyi, NK alt gruplarının flow sitometri ile tayini, sitokin ölçümü yapıldı, litik ünite hesaplandı ve sonuçlar hastaların klinik özellikleri ile birlikte değerlendirildi. SPSS 14.0 ile “oneway ANOVA” testi kullanılarak analiz edildi. Bulgular: Grup 2’nin 1: 1 E: H oranında NK sitotoksisitesi diğer gruplardan anlamlı derecede yüksekti p

Anahtar Kelimeler

• Akut lenfoblastik lösemi,kemoterapi,NK sitotoksisitesi,NK reseptörleri,sitokinler

The Impact of Chemotherapy on Natural Killer Cells in Children with Acute Lymphoblastic Leukemia

Öz

Objective: Chemotherapy may induce changes in NK cells which are important componnets of the immune system in children with acute lymphoblastic leukemia who are are on chemotherapy or have completed therapy.Methods: Thirty three children aged between 2 and 19 were included in the study. Their immunophenotypes, related risk groups, and clinical characteristics were recorded. According to ALL TRALL 2000 protocol patients were classified into three groups: Group 1 patients who fulfilled requirements of protocol M , Group 2 patients still on maintenance chemotherapy and Group 3 patients who completed therapy . Control group group 4 consisted of eleven healthy children aged between 2 and 13. NK cytotoxicity test, flow cytometric analysis of NK subgroups, cytokine analysis were performed in blood samples taken with informed consent from parents of the patients and the controls. Clinical characteristics of the patients were evaluated based on these results. Analysis was performed with “one way ANOVA” statistical test using SPSS 14.0. Results: In 1: 1 E: T ratio, Group 2’s NK cytotoxicity was significantly higher than any other group p

Anahtar Kelimeler

• Acute lymphoblastic leukemia,chemotherapy,NK cytotoxicity,NK receptors,cytokines

Kaynakça

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