Frequency of Rotavirus and Adenovirus in Children with Diarrhea

Öz

ABSTRACT Aim: The aim of this study is retrospective evaluation of the frequency of rotavirus and adenovirus in stool, and their distribution according to gender, age and seasons in children with diarrhea admitted at outpatients or hospitalized in our hospital. Material and Methods: Stool samples of patients aged between 0-18 years received at the Medical Microbiology Laboratory between 2021–2022 were evaluated for rotavirus–adenovirus. Rotavirus and adenovirus antigens were determined qualitatively by immunochromatographic cassette test method. Chi-square test was used in the statistical analysis. Results: A total of 1148 stool samples of pediatric patients were received by our laboratory during these two years for rotavirus and adenovirus antigen test. Of 1148 patients, 8.6% were positive for rotavirus, 5.1% for adenovirus, and 0.6% for both rotavirus and adenovirus antigens. Rotavirus and adenovirus was positive in 7.2% and 5.6% of males, respectively, and 10.6% and 4.5% of females, and there were no statistically significant differences. Rotavirus was most frequently found in the age group 3-5 years (11.6%) and adenovirus was most frequently found in the age group 6-9 years (8.4%), with no significant difference. Rotavirus was most frequently detected in spring (12.9%) while adenovirus was found most frequently in winter (8.1%), without significant differences. Antigen positivity was 4.1% and 4.9% in outpatients for rotavirus and adenovirus, respectively, and 15.1% and 5.5% in hospitalized patients. Rotavirus positivity was significantly higher in hospitalized patients than outpatients, and adenovirus positivity did not show a significant difference. Conclusion: We found that rotavirus and adenovirus were significant agents causing diarrhea in children, especially those younger than 5 years old, and that its frequency increased in winter and spring, and as rotavirus is a cause of hospitalization, implementation of rotavirus vaccine into routine vaccination programs seem to be beneficial for patients.

Anahtar Kelimeler

• rotavirus
• adenovirus
• children
• diarhhea

İshal Şikayeti ile Hastaneye Başvuran Çocuklarda Rotavirus ve Adenovirus Sıklığı

Öz

Amaç: Bu çalışmada, hastanemize ishal şikayetiyle ayaktan başvuran ya da yatış yapılan çocuk hastalarda rotavirus ve adenovirus sıklığının belirlenmesi, cinsiyet, yaş ve mevsimlere göre dağılımının retrospektif olarak irdelenmesi amaçlanmıştır. Gereç-Yöntem: Tıbbi Mikrobiyoloji Laboratuvarına, 2021-2022 tarihleri arasında gelen, 0-18 yaş aralığındaki hastalara ait gaita örnekleri rotavirus-adenovirus açısından değerlendirildi. Rotavirus ve adenovirus antijenleri immünokromatografik kaset test yöntemi ile kalitatif olarak saptanmıştır. İstatistiksel analizde Ki-Kare testi kullanıldı. Bulgular: İki yıllık sürede rotavirus ve adenovirus antijen testi birlikte istenen toplam 1148 çocuk hastaya ait gaita numunesi laboratuvarımıza ulaşmıştır. 1148 hastanın %8.6’sında rotavirus, %5.1’inde adenovirus, %0.6’sında hem rotavirus hem de adenovirus antijeni pozitif saptanmıştır. Rotavirus ve adenovirus pozitifliği erkeklerde 7.2 ve %5.6, kızlarda 10.6 ve %4.5 oranında saptanmış, istatistiksel olarak anlamlı fark bulunmamıştır. Rotavirus en sık 3-5 yaş (%11.6), adenovirus ise 6-9 yaş (%8.4) grubunda tespit edilmiş, istatistiksel olarak anlamlı fark saptanmamıştır. Rotavirus en sık ilkbaharda (%12.9) saptanırken adenovirus kış (%8.1) aylarında tespit edilmiş, istatistiksel olarak anlamlı fark bulunmamıştır. Antijen pozitiflik oranı rotavirus ve adenovirus için sırasıyla ayaktan hastalarda %4.1 ve %4.9, yatan hastalarda %15.1 ve %5.5 olarak tespit edilmiştir. Rotavirus pozitiflik oranı yatan hastalarda ayaktan hastalara göre istatistiksel olarak anlamlı yüksek bulunmuş, adenovirus için iki grup arasında istatistiksel olarak anlamlı fark saptanmamıştır. Sonuç: Çalışmamızda çocuklarda, özellikle beş yaşın altında, rotavirus ve adenovirusun önemli birer ishal etkeni olduğu, kış ve ilkbahar aylarında sıklıklarının arttığı, rotavirusun hastaneye yatışlara neden olduğu için aşısının rutin aşılama programına dahil edilmesinin hasta yararına olacağı tespit edilmiştir.

Anahtar Kelimeler

• rotavirus
• adenovirus
• ishal
• çocuklar

Kaynakça

1. 1. Bayrak R, Ünsal A. Retrospective Evaluation of the Prevalence of Rotavirus in Pediatric Patients Performing Stool Examination in a Hospital. KAEÜ Sagl Bil Derg 2022;6(1):47-52.
2. 2. Ndifor F, Lawane AI, Ngam-Asra N, Adoum MA, Otchom BB, Alio HM. The Prevalence of Rotavirus and Adenovirus in Children 0-5 years old Suffering from Acute Diarrhea in the University Hospital Center of Mother and Child (UHC-MC) in N’Djamena, Chad. IJSCIA 2021;6(2):964-68.
3. 3. Shams S, Tafaroji J, Aghaali M, et al. Prevalence of enteric adenovirus and co-infection with rotavirus in children under 15 years of age with gastroenteritis in Qom, Iran. Gastroenterol Hepatol Bed Bench 2022;15(3):256-262.
4. 4. Gunduz A. Prevalence of rotavirus-adenovirus positivity: 5-year retrospective evaluation, Journal of MTU 2022;1(2):50-53.
5. 5. Ozkan EA, Yesilyurt E, Cilsal Z, Yilmaz N, Ozturk O, Sadigov A. Frequency of Rotavirus and Enteric Adenovirus Infection in Children with Acute Gastroenteritis Admitted to Children Outpatient Clinic. Bozok Med J 2020;10(3):61-64.
6. 6. Abdou D, Babacar N, Abdoulaye DT,et al. Prevalence of Rotavirus and Adenovirus in Children with Acute Viral Gastroenteritis in Dakar, Senegal, 2018-2022. Asian J Biol 2023;17(1):16-23.
7. 7. Aydın E, Aydın N, Percin Renders D. Evaluation of the Effect of Acute Gastroenteritis Factors on Laboratory Parameters in Pediatric Patients. FLORA 2022;27(1):125-134.
8. 8. Alp EK, Ozdemir YU. Evaluation of Rotavirus Prevalence in Children with Acute Gastroenteritis: A Single Center Study. Pediatr Pract Res 2022;10(2):78-82.

9. 9. Jasielska M, Kalita B, Kałużny-Czyż M, Grzybowska-Chlebowczyk U. Rotavirus and adenovirus infections and co-infections as a cause of acute gastroenteritis in hospitalized children – a single centre study. Pediatr Pol 2023;98(1):1-4.
10. 10. Genc Bahce Y, Ozudogru O, Acer O. The Frequency Of Rotavirus and Adenovirus in Case Of Gastroenterit Observed in Siirt Region. Gevher Nesibe Journal of Medical & Health Sciences 2022;7(19):23-28.
11. 11. Tüzüner U, Gülcen BS, Özdemir M, Feyzioğlu B. Frequency of Adenovirus and Rotavirus and Their Seasonal Distribution in Children With Gastroenteritis. Klimik Derg 2016;29(3):121-4.
12. 12. Altindis M, Kucukkurt S, Kalaycı R, Aslan FG, Bukulmez A, Yoldas Y. The Frequency Of Rotavirus, Enteric Adenovirus And Norovirus In Children With Acute Diarrhea. OTSBD 2016;1(1):1-12.
13. 13. Kizilirmak A, Caliskan E, Temizkan RC. Rotavirus and Adenovirus Frequency in Children with Acute Gastroenteritis. Konuralp Tıp Derg 2017;9(2):35-39.
14. 14. Ustebay S, Ustebay DU, Ertekin O. The Frequency of Adenovirus and Rotavirus for Children with Acute Gastroenteritis. Kafkas J Med Sci 2019; 9(1):6–10.
15. 15. Ozsari T, Bora G, Kaya B, Yakut K. The Prevalence of Rotavirus and Adenovirus in the Childhood Gastroenteritis. Jundishapur J Microbiol. 2016;9(6):e34867,1-5.
16. 16. Kirisci O, Muratdagi G. Frequency of Rotavirus and Enteric Adenovirus Infection in Patients Presenting to a State Hospital with Acute Gastroenteritis. Sakarya Tıp Derg 2019;9(4):585-591.
17. 17. Say Coskun US, Kasap T. Frequency of rotavirus and adenovirus in pediatric patients with acute gastroenteritis. J Contemp Med 2019;9(1):85-88.
18. 18. Dinç HÖ, Taner Z, Özbey D, Gareayaghi N, Sirekbasan S, Kocazeybek BS. The Prevalence of Rotavirus and Adenovirus Childhood Gastroenteritis:data of the University Hospital of Cerrah paşa Medical Faculty Between January 2013 and December 2018. Turk Mikrobiyol Cemiy Derg 2019;49(4):206-11.
19. 19. Öner SZ, Kaleli İ, Demi R M, Mete E, Çalişkan A. Rotavirus and adenovirus prevalence in patients with acute viral gastroenteritis in Denizli, Turkey, 2017-2021. J Med Virol 2022;94(8):3857-62.
20. 20. Tokak S, Uğurcan D, Güzeş EA. Investigation of Adenovirus and Rotavirus Frequency and Seasonal Distribution in Children with Acute Gastroenteritis. KÜ Tıp Fak Derg 2022;24(1):163-170.
21. 21. Sert S, Erayman B. Frequency and Seasonal Distribution of Adenovirus and Rotavirus in Children Diagnosed with Acute Gastroenteritis: A Single Centre Experience. J Contemp Med 2023;13(2):353-359.
22. 22. Gür Vural D, Torun EG, Biyik I, Tanriverdi Cayci Y, Bilgin K, Birinci A. Investigation of Frequency of Rotavirus and Adenovirus in Patients with Acute Gastroenteritis. KÜ Tıp Fak Derg 2022;24(2):289-294.
23. 23. Bayırlı Turan D, Karaarslan F, Kuruoğlu T, Şerefhanoğlu Ş. Yatarak tedavi gerektiren akut ishalli çocukların rotavirüs ve enterik adenovirüs eneksiyonu yönünden değerlendirilmesi. Turkish Pediatr Dis 2020;14:220-4.
24. 24. Keleş Alp E, Ozdemir YU. Evaluation of Rotavirus Prevalence in Children with Acute Gastroenteritis: A Single Center Study. Pediatr Pract Res 2022; 10(2): 78-82
25. 25. Aytaç Ö, Şenol FF, Öner P, et al. Akut gastroenteritli hastalarda Rotavirus ve Adenovirus sıklığı. Turk Hij Den Biyol Derg 2020;77(2):179-184.