Liver Protection of Hydroxytyrosol Mediated by Spexin and TRPM2

Öz

Background/Aim: In the study, the role of Spexin (SPX) and TRPM2 in the protective effect of Hydroxytyrosol (HT) in rats given Corn Syrup was evaluated. Material and Method: The rats were divided into 4 groups (6 rats in each) (Control, HT, Corn Syrup, Corn Syrup +HT). Rats were given 30% Corn Syrup with drinking water for 6 weeks. 4 ml/kg/day liquid containing HT was applied by oral gavage alone and together with Corn Syrup for 6 weeks. Molecular parameters SPX and TRPM2 were examined histopathologically in liver tissue. Results: The SPX levels decreased and the TRPM2 levels increased more in the Corn Syrup given Group than the Control Group. SPX levels increased and TRPM2 levels decreased after HT treatment. In the HT Group only, no differences were detected when compared to the control Group. Conclusion: SPX and TRPM2 may mediate the protective effect of HT on the liver in rats given corn syrup.

Anahtar Kelimeler

• Corn Syrup
• Hydroxytyrosol
• Spexin
• TRPM2

Spexin ve TRPM2'nin Aracılık Ettiği Hidroksitirozolün Karaciğer Koruması

Öz

Amaç: Araştırmada Mısır Şurubu verilen sıçanlarda Hidroksitirozol (HT)'ün koruyucu etkisinde Spexin (SPX) ve TRPM2'nin rolü değerlendirildi. Gereç ve Yöntem: Sıçanlar 4 gruba (her grupta 6 sıçan) ayrıldı (Kontrol, HT, Mısır Şurubu, Mısır Şurubu +HT). Sıçanlara 6 hafta süreyle %30'luk Mısır Şurubu içme suyuyla birlikte verildi. HT içeren 4 ml/kg/gün sıvı, tek başına ve Mısır Şurubu ile birlikte 6 hafta süreyle oral gavajla uygulandı. Karaciğer dokusunda SPX ve TRPM2 moleküler parametreleri histopatolojik olarak incelendi. Bulgular: Mısır Şurubu verilen grupta SPX seviyeleri azaldı ve TRPM2 seviyeleri kontrol grubuna göre daha fazla arttı. HT tedavisinden sonra SPX seviyeleri arttı, TRPM2 seviyeleri ise azaldı. Yalnızca HT grubunda kontrol grubuyla karşılaştırıldığında herhangi bir farklılık tespit edilmedi. Sonuç: SPX ve TRPM2, mısır şurubu verilen sıçanlarda HT'nin karaciğer üzerindeki koruyucu etkisine aracılık edebilir.

Anahtar Kelimeler

• Mısır Şurubu
• Hidroksitirosol
• Spexin
• TRPM2

Kaynakça

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