Metabolik sendromlu sıçanların karaciğerlerinde oksidatif strese karşı kuersetinin olası koruyucu etkisi

Yıl 2019, Cilt 9, Sayı 3, 203 - 208, 30.09.2019

Ayça BİLGİNOĞLU

https://doi.org/10.16899/jcm.575413

Öz

Amaç: Metabolik sendrom (MS), yüksek şeker düzeyi ve insülin direnci ile ilişkilendirilen tip 2 diyabetin tipine bağlıdır. Tiyoredoksin-1 (TRX-1) sitoplazma ve mitokondride yerleşmiştir ve hücresel reaktif oksijen türlerini kontrol eder. Bu çalışmanın amacı, MS sıçanların karaciğerinde, MS ve oksidatif stres arasındaki ilişkiyi ve kuersetinin TRX-1 bağlantılı oksidatif stres üzerindeki etkisini araştırmaktır.

Gereç ve Yöntem: Erkek Wistar sıçanlar  (200-250g ağırlığında)  kullanıldı. Üç gruba ayrıldılar. Kontrol grup, MS grup (935 mM sükroz içeren içme suyu) ve kuersetin uygulanmış  (15 mg/kg/gün, gavaj ile) MS grup. TRX-1 protein seviyesi Western blot ile belirlenmiştir.

Bulgular: MS gruptaki aspartat transaminaz (AST), alanin transaminaz (ALT), laktat dehidrojenaz (LDH) seviyeleri Kon grubu ile karşılaştırıldığında arttı. MS grubundaki toplam-antioksidan-durumu (TAS), süperoksit-dizmutaz (SOD), ve glutatyon-peroksidaz (GSH-Px) seviyeleri Kon grubu ile karşılaştırıldığında azaldı. MS grubundaki toplam-oksidan-durumu (TOS) seviyesi Kon grubu ile karşılaştırıldığında azaldı. MS grubundaki trigliserit, toplam-kolesterol ve LDL-kolesterol Kon grubu ile karşılaştırıldığında arttı. MS grubundaki TRX-1 seviyesi Kon grubu ile karşılaştırıldığında azalırken TRX-1 aktivitesi ise düştü.

Sonuç: Kuersetin tedavisi AST, ALT, LDH, ve TOS seviyelerini azaltırken GSH-Px, SOD, ve TAS seviyelerini artırdı. Ayrıca, yağ profili de kuersetin ile değişti. Sonuç olarak,  Kuersetin tedavisi, MS grubundaki TRX-1 seviyesini ve aktivitesini önemli derecede artırdı. Bu veriler MS karaciğerinde artmış olan oksidatif stresin kuersetin ile azalabildiğini ileri sürmektedir.  

Anahtar Kelimeler

Metabolik sendrom, kuersetin, oksidatif stres, insülin direnci, karaciğer

Possible protective effect of quercetin against oxidative stress in liver from metabolic syndrome rats

Öz

Abstract

Background/Aims:Metabolic syndrome (MS) is linked to type of type 2 diabetes mellitus associated with high glucose level and insulin resistance. Thioredoxin-1 (TRX-1) is localized in the cytoplasm and the mitochondria and controls cellular reactive oxygen species. The purpose of this study is to examine the relation between MS and oxidative stress, and effect of quercetin on oxidative stress via TRX-1 in liver of MS rats.

Methods: Male wistar rats (200-250g in weight) were used. They were divided three groups. Control group, MS group receiving (935 mM sucrose in drinking water) and quercetin treated (15 mg/kg/day, administered by gavage) MS group. Protein level of TRX-1 was determined by Western blot.

Results:Aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), levels increased in MS group as compared with the Con group. Total-antioxidant-status (TAS), superoxide-dismutase (SOD), and glutathione-peroxidase (GSH-Px) levels decreased in MS group when compared to Con group. Total-oxidant-status (TOS) levels increased in MS group as compared with the Con group. Triglycerides, total-cholesterol and LDL-cholesterol increased in MS group when compared with the Con group. TRX-1 level decreased in MS group and TRX-1 activity was lower in MS group than Con group.

Conclusions: Treatment of quercetin decreased AST, ALT, LDH, and TOS levels while it increased GSH-Px, SOD, and TAS levels. Also, lipid profile changed with quercetin. In conclusion, treatment of quercetin significantly increased TRX-1 level and activity of TRX-1 in MS group. These data suggest that elevated oxidative stress in liver of MS may be reduced by quercetin.

Anahtar Kelimeler

Metabolic syndrome, quercetin, oxidative stress, insulin resistance, liver

Kaynakça

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