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The effect of Intermittent fasting on the growth and ghrelin hormone in rats feeding on a standard diet

Yıl 2022, Cilt 12, Sayı 4, 570 - 573, 31.07.2022
https://doi.org/10.16899/jcm.1123443

Öz

Aim: In this study, the effect of intermittent fasting on growth hormone (GH) and ghrelin was examined in rats that fed on a standard diet without any application that may cause the values to decrease or increase in order to see the net effect of intermittent fasting. Materials and Methods: 12 Wistar albino male rats were divided into the 1st group as Control (standard diet 2.8% crude fat. 23.1% crude protein. 5% crude fiber. 7.1% crude ash and 12.8% moisture) and the 2nd group as the intermittent fasting together with the diet group (with a 24-hour break from the non-consecutive diet for 2 days a week and all food restricted except water). Results: As a result of the analysis, it was found that the growth hormone in the intermittent fasting together with the standard diet group tended to increase compared to the control group, and while this value difference was not statistically significant, the ghrelin level was found to be statistically lower than the control group. Conclusion: As a result, it was found that intermittent fasting tends to increase the level of growth hormone, and to have a decreasing effect on ghrelin level, and it was concluded that it can be considered among the methods to be used to treat obesity and prevent its occurrence.

Kaynakça

  • 1.Hill JO, Wyatt HR, Peters JC. Energy balance and obesity. Circulation 2012; 126(1), 126-132.
  • 2.Ganesan K, Habboush Y, Sultan S . Intermittent Fasting: The Choice for a Healthier Life style. Cureus 2018; 10(7), e2947.
  • 3. Panda S. Circadian physiology of metabolism. Science 2016; 354,1008–1015.
  • 4. Wilkinson MJ, Manoogian EN, Zadourian A, et al. Ten-hour time-restricted eating reduces weight, blood pressure, and atherogenic lipids in patients with metabolic syndrome. Cell Metab 2020; 31,92–104.
  • 5. Mattson MP, Longo VD, Harvie M. Impact of intermittent fasting on health and disease processes. Ageing Res Rev 2017; 39, 46–58.
  • 6. de Cabo R, Mattson MP. Effects of intermittent fasting on health, aging, and disease. N Engl J Med 2019; 381,2541–2551.
  • 7. Moro T, Tinsley G, Bianco A, et al. Effects of eight weeks of time-restricted feeding (16/8) on basal metabolism, maximal strength, body composition, inflammation, and cardiovascular risk factors in resistance trained males. J Transl Med 2016; 14(1), 290.
  • 8. Kartinah NT, Sianipar IR, Nafi'ah, and Rabia . The Effects of Exercise Regimens on Irisin Levels in ObeseRats Model: Comparing High-Intensity Intermittent with Continuous Moderate-Intensity Training. Biomed Res Int 2018; 27, 4708287. eCollection.
  • 9. Date Y, Kojima M, Hosoda H, et al. Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans. Endocrinology2000; 141,4255–4261.
  • 10. Al Massadi O, Lopez M, Tschop M, et al. Current understanding of the hypothalamic ghrelin path ways inducing appetite and adiposity. Trends Neurosci 2017; 40,167–180.
  • 11.Veedfald S, Plamboeck A, Hartmann B, et al. Ghrelin secretion in humans—A role for the vagus nerve. Neurogastroenterol Motil 2018; 30:e13295.
  • 12. Wang WM, Li SM, Du FM, et al. Ghrelin and obestatin levels in hypertensive obese patients. J Int Med Res 2014; 42,1202–1208.
  • 13. Briggs DI, Enriori PJ, Lemus MB, et al. Diet-induced obesity causes ghrelin resistance in arcuatenpy/agrpneurons. Endocrinology 2010;151,4745–4755.
  • 14. Perreault M, Istrate N, Wang L, et al. Tozzo E., Stricker-Krongrad A. Resistance to the orexigenic effect of ghrelin in dietary-inducedobesity in mice: Reversal upon weight loss. Int J Obes Relat Metab Disord 2004; 28, 879–885.
  • 15. Natalucci G, Riedl S, Gleiss A, et al. Frisch H. Spontaneous 24-h ghrelin secretion pattern in fasting subjects: maintenance of a meal-related pattern. Eur J Endocrinol 2005;152(6), 845–50.
  • 16. Natheer AR , Mohamed M , Haitham J, et al. Effect of diurnal intermittent fasting during Ramadan on ghrelin, leptin, melatonin, and cortisol levels among overweight and obese subjects: A prospective observational study. PLoSOne 2020;15(8), e0237922.
  • 17. Alzoghaibi MA, Pandi-Perumal SR, Sharif MM, et al. Diurnal intermittent fasting during Ramadan: the effects on leptin and ghrelin levels. PloS One 2014;9(3), e92214.
  • 18. Maria NS, Hui L, Stewart C, et al. Page .The Effect of High-Fat Diet-Induced Obesity on the Expression of Nutrient Chemosensors in the Mouse Stomach and the Gastric Ghrelin Cell. Nutrients 2020; 12 (9), 2493.
  • 19. Baltaci AK, Mogulkoc R, Baltaci SB. Review: The role of zinc in the endocrine system. Pak J Pharm Sci 2019; 32(1), 231-239.
  • 20. Al Aboud AM, Zito PM. StatPearls [Internet]. StatPearls Publishing; Treasure Island (FL) 2020; Sep 29, Alopecia.
  • 21. Mc Callum RW, Petrie JR, Dominiczak AF, et al. Growth hormone deficiency and vascular risk. Clin Endocrinol (Oxf) 2002; 57, 11–24.
  • 22. Salgin B, Marcovecchio ML, Hill N, et al. The effect of prolonged fasting on levels of growth hormone-binding protein and free growth hormone Growth Hormone IGF Research 2012; 22(2),76-81.
  • 23. Norrelund H. The metabolic role of growth hormone in humans with particular reference to fasting, Growth Horm. IGF Res 2005; 15(2), 95–122.
  • 24. Moller N, Jorgensen JO. Effects of growth hormone on glucose, lipid, and protein metabolism in human subjects, Endocr Rev2009; 30 (2), 152–177.
  • 25. Alkén J, Petriczko E, Marcus C. Effect of fasting on young adults who have symptoms Of hypoglycemia in the absence of frequent meals. Eur J Clin Nutr2008; 62,721–726.
  • 26. Beer SF, Bircham PM, Bloom SR, et al. The effect of a 72-h fast on plasma levels of pituitary, adrenal, thyroid, pancreatic and gastrointestinal hormones in healthy men and women. J Endocrinol 1989; 120 (2), 337–350.
  • 27. Salgin B, Marcovecchio ML, Humphreys SM, et al. Effects of prolonged fasting and sustained lipolysis on insülin secretion and insülin sensitivity in normal subjects, Am J Physiol Endocrinol Metab2009; 296 (3), 454–461.
  • 28. Moller N, Porksen N, Ovesen P, et al. Evidence for increased sensitivity of fuel mobilization to growth hormone during short-term fasting in humans, Horm.- Metab Res, 1993;25(3),175–179.
  • 29. Hartman, ML, Veldhuis JD, Johnson ML, et al. Augmented growth hormone (GH) secretoryburst frequency and amplitude mediate enhanced GH secretion during a two-day fast in normal men. J Clin Endocrinol Metab1992; 74, 757–765.

Standart diyet ile beslenen ratlarda aralıklı beslenmenin büyüme ve ghrelin hormonu üzerine etkisi

Yıl 2022, Cilt 12, Sayı 4, 570 - 573, 31.07.2022
https://doi.org/10.16899/jcm.1123443

Öz

Amaç: Bu çalışmada aralıklı beslenmenin net etkisini görebilmek için değerlerin düşmesi veya yükselmesine neden olabilecek herhangi bir uygulama yaptırılmadan standart diyetle beslenme uygulanan ratlarda aralıklı beslenmenin büyüme hormonu (GH) ve ghrelin üzerine etkisi incelendi. Gereç ve Yöntem: 12 adet Wistar albino ırkı erkek rat 1. Kontrol (standart beslenme % 2.8 ham yağ. % 23.1 ham protein. % 5 ham selüloz. % 7.1 ham kül. ve % 12.8 nem) 2. grubumuz ise standart diyet ile birlikte aralıklı beslenme (haftada 2 gün ardışık olmayan diyet verilmesine 24 saat ara verilip su hariç tüm besin kısıtlaması) grubu olarak belirlendi. Bulgular: Yapılan analizler sonucunda standart diyet ile birlikte aralıklı diyet uygulanan gruptaki büyüme hormonu kontrol grubuna göre artma eğiliminde olurken bu değer farkı istatsitiki önemde bulunmazken, ghrelin seviyesinin ise kontrol grubuna göre istatiktiki önemde düşük olduğu bulundu. Öneri: Sonuç olarak aralıklı beslenme uygulamasının büyüme hormonu seviyesini arttırma eğiliminde, ghrelin seviyesi üzerinde ise düşürücü yönde etkisinin olduğu görülmüş olup, obezitenin tedavisinde ve oluşumunu önlemede kullanılacak yöntemler arasında değerlendirilebileceği kanısına varılmıştır.

Kaynakça

  • 1.Hill JO, Wyatt HR, Peters JC. Energy balance and obesity. Circulation 2012; 126(1), 126-132.
  • 2.Ganesan K, Habboush Y, Sultan S . Intermittent Fasting: The Choice for a Healthier Life style. Cureus 2018; 10(7), e2947.
  • 3. Panda S. Circadian physiology of metabolism. Science 2016; 354,1008–1015.
  • 4. Wilkinson MJ, Manoogian EN, Zadourian A, et al. Ten-hour time-restricted eating reduces weight, blood pressure, and atherogenic lipids in patients with metabolic syndrome. Cell Metab 2020; 31,92–104.
  • 5. Mattson MP, Longo VD, Harvie M. Impact of intermittent fasting on health and disease processes. Ageing Res Rev 2017; 39, 46–58.
  • 6. de Cabo R, Mattson MP. Effects of intermittent fasting on health, aging, and disease. N Engl J Med 2019; 381,2541–2551.
  • 7. Moro T, Tinsley G, Bianco A, et al. Effects of eight weeks of time-restricted feeding (16/8) on basal metabolism, maximal strength, body composition, inflammation, and cardiovascular risk factors in resistance trained males. J Transl Med 2016; 14(1), 290.
  • 8. Kartinah NT, Sianipar IR, Nafi'ah, and Rabia . The Effects of Exercise Regimens on Irisin Levels in ObeseRats Model: Comparing High-Intensity Intermittent with Continuous Moderate-Intensity Training. Biomed Res Int 2018; 27, 4708287. eCollection.
  • 9. Date Y, Kojima M, Hosoda H, et al. Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans. Endocrinology2000; 141,4255–4261.
  • 10. Al Massadi O, Lopez M, Tschop M, et al. Current understanding of the hypothalamic ghrelin path ways inducing appetite and adiposity. Trends Neurosci 2017; 40,167–180.
  • 11.Veedfald S, Plamboeck A, Hartmann B, et al. Ghrelin secretion in humans—A role for the vagus nerve. Neurogastroenterol Motil 2018; 30:e13295.
  • 12. Wang WM, Li SM, Du FM, et al. Ghrelin and obestatin levels in hypertensive obese patients. J Int Med Res 2014; 42,1202–1208.
  • 13. Briggs DI, Enriori PJ, Lemus MB, et al. Diet-induced obesity causes ghrelin resistance in arcuatenpy/agrpneurons. Endocrinology 2010;151,4745–4755.
  • 14. Perreault M, Istrate N, Wang L, et al. Tozzo E., Stricker-Krongrad A. Resistance to the orexigenic effect of ghrelin in dietary-inducedobesity in mice: Reversal upon weight loss. Int J Obes Relat Metab Disord 2004; 28, 879–885.
  • 15. Natalucci G, Riedl S, Gleiss A, et al. Frisch H. Spontaneous 24-h ghrelin secretion pattern in fasting subjects: maintenance of a meal-related pattern. Eur J Endocrinol 2005;152(6), 845–50.
  • 16. Natheer AR , Mohamed M , Haitham J, et al. Effect of diurnal intermittent fasting during Ramadan on ghrelin, leptin, melatonin, and cortisol levels among overweight and obese subjects: A prospective observational study. PLoSOne 2020;15(8), e0237922.
  • 17. Alzoghaibi MA, Pandi-Perumal SR, Sharif MM, et al. Diurnal intermittent fasting during Ramadan: the effects on leptin and ghrelin levels. PloS One 2014;9(3), e92214.
  • 18. Maria NS, Hui L, Stewart C, et al. Page .The Effect of High-Fat Diet-Induced Obesity on the Expression of Nutrient Chemosensors in the Mouse Stomach and the Gastric Ghrelin Cell. Nutrients 2020; 12 (9), 2493.
  • 19. Baltaci AK, Mogulkoc R, Baltaci SB. Review: The role of zinc in the endocrine system. Pak J Pharm Sci 2019; 32(1), 231-239.
  • 20. Al Aboud AM, Zito PM. StatPearls [Internet]. StatPearls Publishing; Treasure Island (FL) 2020; Sep 29, Alopecia.
  • 21. Mc Callum RW, Petrie JR, Dominiczak AF, et al. Growth hormone deficiency and vascular risk. Clin Endocrinol (Oxf) 2002; 57, 11–24.
  • 22. Salgin B, Marcovecchio ML, Hill N, et al. The effect of prolonged fasting on levels of growth hormone-binding protein and free growth hormone Growth Hormone IGF Research 2012; 22(2),76-81.
  • 23. Norrelund H. The metabolic role of growth hormone in humans with particular reference to fasting, Growth Horm. IGF Res 2005; 15(2), 95–122.
  • 24. Moller N, Jorgensen JO. Effects of growth hormone on glucose, lipid, and protein metabolism in human subjects, Endocr Rev2009; 30 (2), 152–177.
  • 25. Alkén J, Petriczko E, Marcus C. Effect of fasting on young adults who have symptoms Of hypoglycemia in the absence of frequent meals. Eur J Clin Nutr2008; 62,721–726.
  • 26. Beer SF, Bircham PM, Bloom SR, et al. The effect of a 72-h fast on plasma levels of pituitary, adrenal, thyroid, pancreatic and gastrointestinal hormones in healthy men and women. J Endocrinol 1989; 120 (2), 337–350.
  • 27. Salgin B, Marcovecchio ML, Humphreys SM, et al. Effects of prolonged fasting and sustained lipolysis on insülin secretion and insülin sensitivity in normal subjects, Am J Physiol Endocrinol Metab2009; 296 (3), 454–461.
  • 28. Moller N, Porksen N, Ovesen P, et al. Evidence for increased sensitivity of fuel mobilization to growth hormone during short-term fasting in humans, Horm.- Metab Res, 1993;25(3),175–179.
  • 29. Hartman, ML, Veldhuis JD, Johnson ML, et al. Augmented growth hormone (GH) secretoryburst frequency and amplitude mediate enhanced GH secretion during a two-day fast in normal men. J Clin Endocrinol Metab1992; 74, 757–765.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Bilimleri ve Hizmetleri
Bölüm Orjinal Araştırma
Yazarlar

Nizamettin GÜNBATAR> (Sorumlu Yazar)
VAN YUZUNCU YIL UNIVERSITY, SCHOOL OF HEALTH
0000-0002-6684-3970
Türkiye


Bahattin BULDUK>
VAN YUZUNCU YIL UNIVERSITY, SCHOOL OF HEALTH
0000-0002-0626-4758
Türkiye

Erken Görünüm Tarihi 1 Haziran 2022
Yayımlanma Tarihi 31 Temmuz 2022
Kabul Tarihi 20 Haziran 2022
Yayınlandığı Sayı Yıl 2022, Cilt 12, Sayı 4

Kaynak Göster

AMA Günbatar N. , Bulduk B. The effect of Intermittent fasting on the growth and ghrelin hormone in rats feeding on a standard diet. J Contemp Med. 2022; 12(4): 570-573.