Green Synthesis, Characterization and Antibacterial Activities of Silk Sericin Capped Zinc Oxide Nanoparticles

Öz

In recent years, interest in metal-based antibacterial materials has increased due to microorganisms gaining resistance to antibiotics. Silk sericin obtained from Bombyx mori cocoon has found use in many different areas thanks to its biocompatibility, hydrophilic character and biodegradability. Zinc oxide nanoparticles (ZnONPs) obtained in various zinc salts exhibit broad-spectrum antibacterial properties. In this study, to be produce metal based antibacterial materials, synthesis of silk sericin-coated ZnONPs (SS-ZnONPs) in a green and scalable method was investigated by using silk sericin protein as both reducing and capping agent to obtain ZnONPs. For producing SS-ZnONPs, 2% silk sericin solution was mixed with Zn(NO3)2 solution and the blend solution was heated at 100 °C for a certain period of time. Observing surface plasmon resonance (SPR) peak specific at 380 nm in the UV-vis spectrum of SS-ZnONPs represented the formation of ZnONPs. Then, the chemical, morphological, crystalline, thermal, and antibacterial properties of the synthesized SS-ZnONPs were examined. Characteristic peak of the Zn-O band was found in fourier transform infrared spectroscopy (FTIR) analysis of SS-ZnONPs. According to scanning electron microscopy (SEM) analyses, ZnONPs had morphology similar to cubic/hexagonal shape, showed a uniform structure, and did not represent any agglomerations. In energy dispersive spectroscopy (EDS) analyses of SS-ZnONPs, peaks belonging to carbon, nitrogen, oxygen, sulphur, and zinc elements were observed. The formation of Zn peak indicated that the zinc ions were transformed into ZnONPs. In addition, characteristic peaks of zinc were seen in the X-ray diffractometer (XRD) result of SS-ZnONPs. Thermogravimetric analysis (TGA) showed that the thermal stability and remaining amount of SS-ZnONPs was higher compared to pure silk sericin powder due to the formation of ZnONPs. Lastly, agar well diffusion test was carried out with Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC 25922) bacteria and SS-ZnONPs showed antibacterial action against S. aureus. It has been observed that the obtained SS-ZnONPs can be used as antibacterial agents. However, it was also understood that the ZnONPs concentration in this study was low for high antibacterial activity.

Anahtar Kelimeler

• Green synthesis
• Silk sericin
• Zinc oxide nanoparticles
• Antibacterial materials

İpek Serisin Kaplı Çinko Oksit Nanopartiküllerinin Yeşil Sentezi, Karakterizasyonu ve Antibakteriyel Aktiviteleri

Öz

Son yıllarda mikroorganizmaların antibiyotiklere karşı direnç kazanması nedeniyle metal bazlı antibakteriyel malzemelere olan ilgi artmıştır. Bombyx mori kozasından elde edilen ipek serisin, biyouyumluluğu, hidrofilik karakteri ve biyolojik olarak parçalanabilirliği sayesinde birçok farklı alanda kullanım alanı bulmuştur. Çeşitli çinko tuzlarından elde edilen çinko oksit nanopartikülleri (ZnONP’ler), geniş spektrumlu antibakteriyel özellikler sergilemektedir. Bu çalışmada, metal bazlı antibakteriyel malzeme üretmek amacıyla, ZnONP elde etmek için ipek serisin proteini hem indirgeyici hem de kaplayıcı madde olarak kullanılarak yeşil ve ölçeklenebilir bir yöntemle ipek serisin kaplı ZnONP’lerin (SS-ZnONP’ler) sentezi araştırılmıştır. SS-ZnONP'lerin üretimi için %2’lik ipek serisin çözeltisi Zn(NO3)2 çözeltisi ile karıştırılmış ve karışım çözeltisi 100 °C’de belirli bir süre ısıtılmıştır. SS-ZnONP’lerin UV-vis spektrumunda 380 nm’de spesifik yüzey plazmon rezonansı (SPR) zirvesinin gözlemlenmesi, ZnONP’lerin oluşumunu göstermektedir. Daha sonra sentezlenen SS-ZnONP’lerin kimyasal, morfolojik, kristal, termal ve antibakteriyel özellikleri incelenmiştir. Zn-O bandının karakteristik zirvesi, SS-ZnONP’lerin fourier transform kızılötesi spektroskopisi (FTIR) analizinde görülmüştür. Taramalı elektron mikroskobu (SEM) analizlerine göre ZnONP’lerin kübik/altıgen şekle benzer bir morfolojiye sahip olduğu, tekdüze bir yapı gösterdiği ve herhangi bir topaklanma göstermediği görülmüştür. SS-ZnONP’lerin enerji dağılımlı spektroskopi (EDS) analizlerinde karbon, azot, oksijen, kükürt ve çinko elementlerine ait pikler görülmüştür. Zn zirvesinin oluşması çinko iyonlarının ZnONP’lere dönüştüğünü göstermektedir. Ek olarak, SS-ZnONP’lerin X-ışını difraktometresi (XRD) sonucunda karakteristik çinko zirveleri vardır. Termogravimetrik analiz (TGA), ZnONP oluşumu nedeniyle SS-ZnONP’lerin termal stabilitesinin ve kalan miktarının saf ipek serisin tozuna kıyasla daha yüksek olduğunu göstermektedir. Son olarak Staphylococcus aureus (ATCC 6538) ve Escherichia coli (ATCC 25922) bakterileri ile agar kuyucuk difüzyon testi yapılmış ve SS-ZnONP’ler S. aureus’a karşı antibakteriyel etki göstermiştir. Elde edilen SS-ZnONP’lerin antibakteriyel ajan olarak kullanılabileceği görülmüştür. Ancak bu çalışmada ZnONP konsantrasyonunun yüksek antibakteriyel aktivite nedeniyle düşük olduğu da anlaşılmıştır.

Anahtar Kelimeler

• Yeşil sentez
• İpek serisin
• Çinko oksit nanopartikülleri
• Antibakteriyel malzemeler

Kaynakça

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