Assessing Antiviral Mechanisms of N-acetyl-D-glucosamine, N-acetylcysteine and Acetylsalicylic acid on SARS-CoV-2

Öz

The potential antiviral activity of FDA-approved 3 compounds (N-acetyl-D-glucosamine; GlcNAc, N-acetylcysteine; NAC, and Acetylsalicylic acid; ASA) against SARS-CoV-2 was investigated. Molecular docking analysis of these compounds as ligands with the main 22 viral proteins was made to predict their possible interaction. All molecules showed interactions with the viral proteins; the mean binding scores for GlcNAc and ASA were very close (-6.81 kcal/mol and -6.31 kcal/mol, respectively), while NAC designated the lowest value (-4.69 kcal/mol). GlcNAc showed the highest binding energy of -8.80 kcal/mol against both the target proteins RdRp-RTP site (7BV2) and Helicase-ANP binding site (7NN0). Since these 22 proteins, including main protease (Mpro) and Papain-like protease (PLpro), are responsible for replication and various pathogenesis processes, it could be concluded that these FDA-approved commercially available compounds have antiviral properties against SARS-CoV-2, which should be confirmed with further in vivo and clinical trials.

Anahtar Kelimeler

• bioinformatics
• ligand
• molecular docking
• nCoV
• repurposing
• SARS-CoV-2

N-asetil-D-glukozamin, N-asetilsistein ve Asetilsalisilik asidin SARS-CoV-2’ye Antiviral Mekanizmalarının Değerlendirilmesi

Öz

FDA onaylı 3 bileşiğin (N-asetil-D-glukozamin; GlcNAc, N-asetilsistein; NAC ve Asetilsalisilik asit; ASA) SARS-CoV-2'ye karşı potansiyel antiviral aktivitesi araştırılmıştır. Bu bileşiklerin ligand olarak 22 belli başlı viral proteinle moleküler kenetlenme analizleri yapılarak olası etkileşimleri tahmin edilmiştir. Tüm moleküller viral proteinlerle etkileşim göstermiştir; GlcNAc ve ASA için ortalama bağlanma skorları birbirine çok yakın çıkmıştır (-6.81 kcal/mol ve -6.31 kcal/mol, sırasıyla), NAC ise en düşük değeri göstermiştir (-4.69 kcal/mol). GlcNAc, hedef proteinler olan hem RdRp-RTP bölgesine (7BV2) hem de Helikaz-ANP bağlanma bölgesine (7NN0) -8.80 kcal/mol ile en yüksek bağlanma enerjisini göstermiştir. Bu 22 protein arasında ana proteaz (Mpro) ve Papain-benzeri proteaz (PLpro) gibi replikasyon ve çeşitli patogenez süreçlerinden sorumlu proteinler yer aldığından, bu FDA onaylı ve ticari olarak mevcut bileşiklerin SARS-CoV-2'ye karşı antiviral özelliklere sahip olduğu, ancak bunun daha ileri in vivo ve klinik çalışmalarla doğrulanması gerektiği sonucuna varılmıştır.

Anahtar Kelimeler

• Biyoenformatik
• ligand
• moleküler kenetlenme
• nCoV
• yeniden amaçlandırma
• SARS-CoV-2

Kaynakça

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