İstiridye Mantarı ve Lipozomal Formu: Antimikrobiyal Özellikler Açısından Herhangi Bir Farklılık Var mı?

Öz

İstiridye mantarı ve lipozomal formunun karşılaştırıldığı bir çalışmaya literatürde rastlanmadığından, bu çalışmada istiridye mantarı ve lipozomal formunun antibakteriyel aktiviteleri karşılaştırılmıştır. Pleurotus ostreatus'un metanolik ekstresi ve lipozomal formu hazırlanmıştır. Ardından antibakteriyel özellikler Escherichia coli ATCC 25922, Enterococcus faecium ATCC 8459, Streptococcus agalactiae ATCC 12386, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Staphylococcus epidermidis ATCC 12228 ve Salmonella typhimurium ATCC 14028'e karşı in vitro koşullar altında disk difüzyon yöntemi ile tespit edilmiştir. İstiridye mantarı ekstresi ve lipozomal formunun antibakteriyel özellikleri karşılaştırıldığında, lipozomal formun inhibisyon zon çaplarının Escherichia coli, Streptococcus agalactiae, Staphylococcus aureus, Staphylococcus epidermidis ve Salmonella typhimurium için daha büyük olduğu gözlemlenmiştir. Ayrıca, Pleurotus ostreatus'un metanolik ekstresi Staphylococcus epidermidis ve Salmonella typhimurium'a karşı antibakteriyel aktiviteye sahip olmamasına rağmen, lipozomal formu bunlara karşı antibakteriyel aktivite sergilemiştir. Sonuçlarımız, daha ileri araştırmalar için 2 önemli perspektifle literatüre katkıda bulunabilir. Bunlardan birisi, antibakteriyel ajanların lipozomal formları sayesinde daha etkili olabileceğidir. Diğer ilginç bir bakış açısı ise, normalde antibakteriyel özelliği olmadığı bilinen maddelerin lipozomal formlarının antibakteriyel özelliklere sahip olabileceğinin göz önünde bulundurulabilmesidir.

Anahtar Kelimeler

• İstiridye mantarı
• Antibakteriyel ajanlar
• Pleurotus ostreatus
• Lipozomal form
• Nanoteknoloji

The Oyster Mushroom and Its Liposomal Form: Is There Any Differences in Terms of The Antimicrobial Properties?

Öz

As there has been no study comparing oyster mushroom and its liposomal form in the literature, antibacterial activities of oyster mushroom and its liposomal form were compared in this study. The methanolic extract of Pleurotus ostreatus and its liposomal form were prepared. Then, the antibacterial properties were found out under in vitro conditions against Escherichia coli ATCC 25922, Enterococcus faecium ATCC 8459, Streptococcus agalactiae ATCC 12386, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Staphylococcus epidermidis ATCC 12228, and Salmonella typhimurium ATCC 14028 by disc diffusion method. When the antibacterial properties of oyster mushroom extract and its liposomal form were compared, it was observed that the inhibition zone diameters of the liposomal form were larger for Escherichia coli, Streptococcus agalactiae, Staphylococcus aureus, Staphylococcus epidermidis, and Salmonella typhimurium. Additionally, although the methanolic extract of Pleurotus ostreatus has no antibacterial activity against Staphylococcus epidermidis and Salmonella typhimurium, its liposomal form exhibited antibacterial activity against them. Our results can contribute to literature with 2 important perspectives for further research. One of them is that the antibacterial agents can be more effective thanks to liposomal forms of them. The other interesting point of view is that it can be taken into consideration liposomal forms of substances that are normally known to have no antibacterial properties of their extracts may have antibacterial properties.

Anahtar Kelimeler

• Oyster mushroom
• Antibacterial agents
• Pleurotus ostreatus
• Liposomal form
• Nanotechnology

Kaynakça

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