EFFECT OF CO2 CONCENTRATION AND NITROGEN STRESS ON CO2 CAPTURE EFFICIENCY OF CHLORELLA VULGARIS MICROALGE CULTURE

Öz

CO2 capture in the flue gas by microalgae is important in terms of gloabal warming. The effect of CO2 concentration and nitrogen stress on CO2 capture efficiency of Chlorella vulgaris microalgae culture in this study. A photobioreactor with 5 cm inner diameter and 100 cm height was used for the tests. CO2 concentration that gives the best results in terms of CO2 capture efficiency was determined and under this condition, microalgae culture was exposed to nitrogen stress and the lipid ratio in its structure was examined. The maximum growth was achieved at 15% CO2. The growth was hindered when CO2 was increased to 90%. At high CO2, the medium should be buffered and CO2 should be gradually increased. The growth was inhibited at 15% CO2 under nitrogen stress in the photobioreactor. Therefore, nitrogen stress tests (100% ve 75%) were conducted in an erlenmeyer flask at 400 ppmv CO2. It was determined that there was a better growth under 75% nitrogen stress (1.3 times higher) compared to 100% nitrogen stress. Moreover, RuBisCO for 75% nitrogen stress was 2.5 times higher than %100 nitrogen stress. However, lipid content was 1.2 times higher for 100% nitrogen stress compared to the 75% nitrogen stress.

Anahtar Kelimeler

• CO2 Capture
• Microalgae
• Flue Gas
• Nitrogen Stress
• Biodiesel

CO2 DERİŞİMİNİN VE AZOT STRESİNİN CHLORELLA VULGARİS MİKROALG KÜLTÜRÜNÜN CO2 TUTMA VERİMİNE ETKİSİ

Öz

Baca gazındaki CO2 gazının mikroalglerle tutulması küresel ısınmayla mücadele bakımından önemlidir. Bu çalışmada; farklı CO2 derişimlerinin (hacimce 400 ppm, %15 ve %90) ve azot stresinin Chlorella vulgaris mikroalg kültürünün CO2 tutma verimine etkisi incelenmiştir. Çalışmada, 5 cm iç çapa sahip, 100 cm yüksekliğinde pleksiglastan yapılmış fotobiyoreaktör kullanılmıştır. CO2 tutma verimi açısından en iyi sonucu veren CO2 derişimi belirlenmiş ve bu şartlar altında, mikroalg kültürü azot stresine maruz bırakılarak bünyesindeki lipit oranının nasıl değiştiği incelenmiştir. Mikroalg kültürü için en iyi büyüme değerleri %15 CO2 içeren gaz karışımı altında elde edilmiştir. Fotobiyoreaktöre verilen gaz karışımındaki CO2 oranı %90’a çıkarıldığında alg hücrelerinin inhibe olduğu gözlemlenmiştir. Yüksek CO2 derişimlerinde ortamın tamponlanması ve ortama verilen CO2 derişiminin kademeli olarak arttırılması gerekmektedir. %15 CO2 derişiminde %100 azot stresinde fotobiyoreaktörde büyüme gerçekleşmemiştir. Bu nedenle azot stresi (%100 ve %75) deneylerine erlenmeyerde 400 ppmv CO2 derişimi altında devam edilmiştir. %75 azotsuz ortamda %100 azotsuz ortama göre 1,3 kat daha iyi büyüme verimi elde edilmiştir. Ayrıca, %75 azotsuz deneyde %100 azotsuz ortama göre 2,5 kat daha yüksek RuBisCO oranı belirlenmiştir. %100 azotsuz deneyde ise %75 azotsuz ortama göre 1,2 kat daha yüksek lipit oranı elde edilmiştir.

Anahtar Kelimeler

• CO2 Tutma
• Mikroalg
• Baca Gazı
• Azot Stresi
• Biyodizel

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