SYNTHESIS OF ANTIBACTERIAL PVA-AAM PICKERING EMULSION HYDROGELS (PEHs) FOR MEDICAL APPLICATIONS

Öz

Citronella essential oil (CEO) has antibacterial characteristics that can be used in medical applications. Polyvinyl alcohol/acrylamide (PVA/AAm) hydrogels including CEO were stabilized utilizing beta-cyclodextrin (β-CD) carbonhydrate-based material. In this context, PVA polymer, and AAm monomer were used as matrix (water phase) whereas CEO and (oil phase) and carbohydrate-based β-cyclodextrin (β-CD) were used in the emulsion system as an active agent and an emulsifier, respectively. The β-CD/CEO inclusion complexes were introduced to PVA/AAm hydrogels in different ratios (1:0.25, 1:0.5, and 1:1% w/v), and to obtain Pickering hydrogels by combining UV and freeze-thawing and anneal-swelling methods, respectively. The influence of different β-CD/CEO ratios on the morphological, physical, hydrophilicity and antibacterial efficiency of PVA/AAm was studied, as well. Although the amount of CEO increased, the hydrophilicity of hydrogels increased, while the swelling properties of hydrogels decreased. Moreover, the hydrogel with the highest amount of CEO showed the highest value of antibacterial activity against both Escherichia coli and Staphylococcus aureus bacteria. The resulting Pickering hydrogels (PEHs) can be a potential use as tissue expander for many medical field.

Anahtar Kelimeler

• PVA/AAm Polymer Blend
• Pickering Emulsion Hydrogels(PEHs)
• Medical Applications
• Citronella Essential Oil(CEO)
• Antibacterial Efficiency

EKLENECEK

Öz

Sitronella esansiyel yağı (CEO) tıbbi uygulamalarda kullanılabilecek antibakteriyel özelliklere sahiptir. CEO içeren polivinil alkol/akrilamid (PVA/AAm) hidrojeller karbonhidrat bazlı beta-siklodekstrin (β-CD) malzeme ile stabilizeedilmiştir. Bu kapsamda, emülsiyon sisteminde sırasıyla matris (su fazı) olarak PVA ve AAm, CEO etken madde (yağ fazı) ve emülgatör olarak karbonhidrat bazlı β-siklodekstrin (β-CD) kullanılmıştır. β-CD/CEO inklüzyon kompleksleri, PVA/AAm hidrojellerine sırasıyla farklı oranlarda (1: 0.25, 1:0.5 ve 1: 1 w/v) eklenmiştir ve UV ve donma-çözülme ve tavlama-şişme yöntemlerini birleştirerek Pickering hidrojelleri elde edilmiştir. Farklı β-CD/CEO oranlarının PVA/AAm'nin morfolojik, fiziksel, hidrofilikliği ve antibakteriyel etkinliği üzerindeki etkisi de incelenmiştir. CEO miktarı artmasına rağmen hidrojellerin hidrofilikliği artarken hidrojellerin şişme özellikleri azalmıştır. Ayrıca, en yüksek CEO miktarına sahip hidrojel, hem Escherichia coli hem de Staphylococcus aureus bakterilerine karşı en yüksek antibakteriyel aktivite değerini göstermiştir. Elde edilen Pickering hidrojellerin (PEHs), tıp alanı için doku genişletici olarak potansiyel bir kullanıma olabilmektedir.

Anahtar Kelimeler

• PVA/AAm Polimer Karışımı
• Pickering Emülsiyon Hidrojeller
• Medikal Uygulamalar
• s-Sitronella Uçucu Yağı (CEO)
• Antibakteriyal Etkinlik

Kaynakça

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