A Determination Method of Dopamine or Epinephrine by Conducting Polymers : The Electrochemical Impedance Spectroscopy

Öz

In this study, it was reported that the preparation and characterization of capacitive sensor based on conducting polymer for detection of dopamine and/or epinephrine by impedimetric method, electrochemical impedance spectroscopy. Pt electrodes coated with poly(Cz) and poly(In) in different electrolyte solution by electropolymerization. NaClO4-ACN electrolyte solution was selected as suitable electrolyte solution, due to high capacitive behavior. The usage of EIS technique for the determination of DA or EP in presence of AA was investigated. For this reason, the measurement EIS of prepared polymer coated electrodes in suitable conditions was performed by immersed in different concentrations (from 10^-9M to 10^-6M) of DA or EP in presence of 10^-4 M of AA at pH 7. It was found that Poly(Cz) coated electrode was a more convenient biosensor for determination of EP by EIS method than Poly(In) and for DA determination. Also, the EIS method was able to use as the readout of Poly(Cz) coated electrode as a biosensor for the determination of EP.   

Anahtar Kelimeler

• Electro polymerization,Determination,Dopamine,Epinephrine and Impedance .

Kaynakça

1. [1] Jaber, Mohamed, et al. "Dopamine receptors and brain function." Neuropharmacology 35.11 (1996): 1503-1519.
2. [2] Hernandez, P., et al. "Cyclic voltammetry determination of epinephrine with a carbon fiber ultramicroelectrode." Talanta 46.5 (1998): 985-991.
3. [3] Banks, William A. "Enhanced leptin transport across the blood–brain barrier by α1-adrenergic agents." Brain research 899.1 (2001): 209-217.
4. [4] Peskind, Elaine R., et al. "Cerebrospinal fluid epinephrine in Alzheimer's disease and normal aging." Neuropsychopharmacology 19.6 (1998): 465-471.
5. [5] Hows, M.E.. "High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples". J Neurosci Methods, 138(1-2), 123-32., (2004).
6. [6] Sorouraddin, M. H., et al. "Spectrophotometric determination of some catecholamine drugs using sodium bismuthate." Journal of pharmaceutical and biomedical analysis 18.4 (1998): 877-881.
7. [7] Zhang, Liyao, et al. "Determination of dopamine in single rat pheochromocytoma cell by capillary electrophoresis with amperometric detection." Journal of Chromatography B 792.2 (2003): 381-385.
8. [8] He, Maofang, and Xiaohui Zheng. "A highly sensitive and selective method for dopamine detection based on poly (folic acid) film modified electrode." Journal of Molecular Liquids 173 (2012): 29-34.

9. [9] Wang, Ying, et al. "Application of graphene-modified electrode for selective detection of dopamine." Electrochemistry Communications 11.4 (2009): 889-892.
10. [10] Kim, Yang-Rae, et al. "Electrochemical detection of dopamine in the presence of ascorbic acid using graphene modified electrodes." Biosensors and Bioelectronics 25.10 (2010): 2366-2369.
11. [11] Adekunle, Abolanle S., et al. "Electrochemical Response and Impedimetric Behaviour of Dopamine and Epinephrine at Platinum Electrode Modified with Carbon Nanotubes-Gold Nanocomposite." Int. J. Electrochem. Sci 6 (2011): 2826-2844.
12. [12] Li, Fenghua, et al. "Synthesis of Pt/ionic liquid/graphene nanocomposite and its simultaneous determination of ascorbic acid and dopamine." Talanta 81.3 (2010): 1063-1068..
13. [13] Sun, Chia-Liang, et al. "The simultaneous electrochemical detection of ascorbic acid, dopamine, and uric acid using graphene/size-selected Pt nanocomposites." Biosensors and Bioelectronics 26.8 (2011): 3450-3455.
14. [14] Ates, Murat, et al. "A Study of the electrochemical behavior of poly [N-vinyl carbazole] formed on carbon-fiber microelectrodes and its response to dopamine." IEEE Sensors Journal 8.10 (2008): 1628-1639.
15. [15] Ates, Murat, et al. "Carbon fiber microelectrodes electrocoated with polycarbazole and poly (carbazole-co-p-tolylsulfonyl pyrrole) films for the detection of dopamine in presence of ascorbic acid." Microchimica Acta 160.1-2 (2008): 247-251.
16. [16] Sezer, Esma, Özlem Yavuz, and A. Sezai Saraç. "N‐Vinylcarbazole‐Acrylamide Copolymer Electrodes Electrochemical Response to Dopamine." Journal of The Electrochemical Society 147.10 (2000): 3771-3774.
17. [17] Bott, Adrian W. "Electrochemical techniques for the characterization of redox polymers." Current Separations 19.3 (2001): 71-75.
18. [18] Katz, Eugenii, and Itamar Willner. "Probing biomolecular interactions at conductive and semiconductive surfaces by impedance spectroscopy: routes to impedimetric immunosensors, DNA‐sensors, and enzyme biosensors." Electroanalysis 15.11 (2003): 913-947.
19. [19] Guan, Jian-Guo, Yu-Qing Miao, and Qing-Jie Zhang. "Impedimetric biosensors." Journal of bioscience and bioengineering 97.4 (2004): 219-226.
20. [20] Sezgintürk, Mustafa Kemal. "A new impedimetric biosensor utilizing vegf receptor-1 (flt-1): Early diagnosis of vascular endothelial growth factor in breast cancer." Biosensors and Bioelectronics 26.10 (2011): 4032-4039.
21. [21] Liu, Shufeng, et al. "Electrochemical DNA biosensor fabrication with hollow gold nanospheres modified electrode and its enhancement in DNA immobilization and hybridization." Biosensors and Bioelectronics 25.7 (2010): 1640-1645.
22. [22] Zhu, Ningning, et al. "Sensitive impedimetric DNA biosensor with poly (amidoamine) dendrimer covalently attached onto carbon nanotube electronic transducers as the tether for surface confinement of probe DNA." Biosensors and Bioelectronics 25.6 (2010): 1498-1503..
23. [23] Macdonald, Digby D. "Reflections on the history of electrochemical impedance spectroscopy." Electrochimica Acta 51.8 (2006): 1376-1388..
24. [24] Dolas, Hacer, and A. Sezai Sarac. "An impedance-morphology study on poly (3-methylthiophene) coated electrode obtained in boron trifluoride diethyl etherate–acetonitrile." Synthetic Metals 195 (2014): 44-53.
25. [25] Dolas, Hacer, and A. Sezai Sarac. "Electrosynthesis of Poly (3-dodecyl thiophene) in Acetonitrile with Boron Trifluoride Diethyl Etherate: The Effect of the Electrolyte on Electrochemical Impedance and Morphology." Journal of The Electrochemical Society 159.1 (2011): D1-D8.
26. [26] Dolas, Hacer, Esma Sezer, and A. Sezai Sarac. "Synthesis, Characterization and Electrochemical Polymerization of a Comonomer Bearing Thiophene and Imidazole: The Comparison of Impedance Behavior on Different Surfaces." ECS Journal of Solid State Science and Technology 5.5 (2016): P211-P217.