A co-culture study to determine the supportive role of probiotics on immune system against cancer cells

Abstract

In this study, two different in vitro co-culture systems were established in order to determine the supportive role of probiotic Lactobacillus acidophilus LA-5 on macrophages when used in combination with doxorubicin in both human- and mouse-origin cancerous cell lines. First, a co-culture study was undertaken using RAW 264.7 mouse macrophage cells that activated by LA-5 against 4T1 mouse triple negative breast adenocarcinoma cells. After the initial study, a second co-culture was performed using THP-1 human monocyte-like cells activated by LA-5 against CaCo-2 human colorectal adenocarcinoma cells. The supplementary potential of LA-5 on the macrophages was evaluated by investigating the viabilities of 4T1 and CaCo-2 cells via an MTT test. The data obtained in the study indicated that the viability of cancerous cells 4T1 and CaCo-2 decreased to ≈40% and ≈30% in the groups that used LPS-activated macrophages stimulated with LA-5 and the combination of LA-5 and doxorubicin (1/2 IC50), respectively. In conclusion, the use of LA-5 could be encouraged as a dietary supplementary for cancer patients due to its superior effects on immune stimulation.

Keywords

• Probiotics
• Lactobacillus acidophilus LA-5
• Immune system
• Cancer cells

References

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