CHROOCOCCUS MINUTUS (KÜTZİNG) NÄGELİ'NİN (CHROOCOCCALES, CYANOBACTERIA) ANTİOKSİDAN, ANTİBAKTERİYEL VE ALFA-GLUKOZİDAZ İNHİBİSYON ÖZELLİKLERİNİN TOPLAM FENOLİK İÇERİĞİ VE İN VİTRO ANALİZİ

Öz

Amaç: Siyanobakteriler, önemli biyolojik aktivitelere sahip yeni biyoaktif madde kaynaklarıdır. Bu çalışmada, bir tatlı su siyanobakterisi olan Chroococcus minutus'un toplam fenolik içeriği (TPC) ve biyoaktif (antioksidan, antidiyabetik ve antibakteriyel) özellikleri incelenmiştir.

Gereç ve Yöntem: C. minutus'un metanol özütü hazırlanarak, TPC, antioksidan ve alfa-glukozidaz inhibisyon özelliklerini belirlemek için farklı biyolojik deneylere tabi tutulmuştur. Antibakteriyel aktivite, tıbbi açıdan önemli çeşitli bakteriyel patojenlere karşı mikrotitre plaka seyreltme deneyi kullanılarak yapılmıştır.

Sonuç ve Tartışma: C. minutus'un TPC'si 11.27 ± 0.14 mg GAE/g'dir. C. minutus'un antioksidan etkinliği, 255 μg/ml IC50 değeri ile güçlü DPPH temizleme aktivitesine sahip olmasıyla karakterize edilir. Alfa-glukozidaz inhibisyon özelliğinin değerlendirilmesi, C. minutus özütünün, akarboza (standart antidiyabetik ilaç) kıyasla 5.50 μg/ml IC50 ile güçlü inhibisyon aktivitesine sahip olduğunu gösterdi. Ek olarak, C. minutus özütü, Bacillus cereus'a, Listeria monocytogenes'e ve Metisiline dirençli Staphylococcus aureus'a karşı güçlü antibakteriyel aktiviteler sergilemiştir. Mevcut araştırma, farmasötik uygulamalarda önemli kullanımı olan aktif metabolitlerin kaynağı olarak C. minutus'un potansiyelini göstermektedir.

Anahtar Kelimeler

• Biyolojik aktivite
• siyanobakteriler
• tatlı su
• Filipinler

TOTAL PHENOLIC CONTENT AND IN VITRO ANALYSIS OF ANTIOXIDANT, ANTIBACTERIAL, AND ALPHA-GLUCOSIDASE INHIBITION PROPERTIES OF CHROOCOCCUS MINUTUS (KÜTZING) NÄGELI (CHROOCOCCALES, CYANOBACTERIA)

Öz

Objective: Cyanobacteria are novel sources of bioactive substances with important biological activities. In this study, the total phenolic content (TPC) and bioactive (antioxidant, antidiabetic, and antibacterial) properties of a freshwater cyanobacteria, Chroococcus minutus were investigated.

Material and Method: Methanol extract of C. minutus were prepared and subjected to different biological assays to determine the TPC, antioxidant, and alpha-glucosidase inhibition properties. The antibacterial activity was done using microtiter plate dilution assay against various medically important bacterial pathogens.

Result and Discussion: C. minutus has a TPC of 11.27 ± 0.14 mg GAE/g. Antioxidant efficiency of C. minutus are characterized by having potent DPPH scavenging activity with IC50 value of 255 μg/ml. Assessment of alpha-glucosidase inhibition property showed that C. minutus extract have potent inhibition activity with IC50 of 5.50 μg/ml as compared to acarbose (standard antidiabetic drug). In addition, C. minutus extract exhibited potent antibacterial activities against Bacillus cereus, Listeria monocytogenes, and Methicillin-resistant Staphylococcus aureus. The current investigation shows the potential of C. minutus as source of active metabolites with important use in pharmaceutical applications.

Anahtar Kelimeler

• Biological activity
• cyanobacteria
• freshwater
• Philippines

Destekleyen Kurum

University of the Philippines Los Banos

Kaynakça

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