QUERCUS MACRANTHERA SUBSP. SYSPIRENSIS (K. KOCH) MENITSKY’İN DAL VE YAPRAK EKSTRELERİNİN FİTOKİMYASAL ANALİZİ VE ANTİBAKTERİYEL AKTİVİTESİ ÜZERİNE BİR ÇALIŞMA

Öz

Amaç: Meşe türleri dünya genelinde geleneksel kullanımı olan tıbbi bitkilerdendir. Tanen bakımından oldukça zengin olan bu türlerin içerdikleri polifenolik bileşikler açısından antibakteriyel ajan olarak potansiyelleri vardır. Bu çalışmada Türkiye için endemik olan Quercus macranthera subsp. syspirensis’in dal ve yapraklarının antibakteriyel potansiyeli ve fitokimyasal içeriği araştırılmıştır.

Gereç ve Yöntem: Bitki materyalleri 2020 yılında Araç'tan (Kastamonu/Türkiye) toplanmıştır. Kurutulmuş ve toz haline getirilmiş dal ve yapraklardan metanol ekstreleri hazırlanmıştır. Antibakteriyel aktivite, minimum inhibisyon konsantrasyonu (MIC) olarak sıvı mikrodilüsyon yöntemiyle, Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 35984, Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Acinetobacter baumannii ATCC 19606 ve Bacillus subtilis ATCC 6633 mikroorganizmaları üzerine test edilmiştir. Ekstrelerin GC-MS analizi, Agilent 5973N dört kutuplu kütle spektrometresi (Agilent, ABD) ile donatılmış bir Agilent 6890 gaz kromatografı kullanılarak yapılmıştır. Bileşikler, numunenin kütle spektrum oranı NIST 2014 Kütle Spektral Kütüphanesinde bulunan verilerle karşılaştırılarak tanımlanmıştır.

Sonuç ve Tartışma: Sonuç olarak, dal ekstrelerinin yaprak ekstrelerinden daha etkili olduğu bulundu ve her iki ekstrenin de en yüksek antibakteriyel aktiviteyi Bacillus subtilis ATCC 6633 suşuna karşı gösterdiği belirlendi. Ekstreler ayrıca diğer test suşları üzerinde değişen konsantrasyonlarda aktivite gösterdi.      

Anahtar Kelimeler

• Antibakteriyel
• GC-MS
• dal
• yaprak
• Quercus macranthera subsp. syspirensis

A STUDY ON PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF QUERCUS MACRANTHERA SUBSP. SYSPIRENSIS (K. KOCH) MENITSKY BRANCH AND LEAF EXTRACTS

Öz

Objective: Oak species are medicinal plants with traditional use around the world. These species, which are very rich in tannins, have potential as antibacterial agents in terms of the polyphenolic compounds content. In this study, the antibacterial potential and phytochemical content of the branches and leaves of Quercus macranthera subsp. syspirensis, which is endemic to Turkey, were investigated.

Material and Method: Plant materials were collected from Araç (Kastamonu/Turkey) in 2020. Methanol extracts were prepared from dried and powdered branches and leaves. The antibacterial activity test was evaluated by broth microdilution method as a minimal inhibition concentration (MIC) against Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 35984, Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Acinetobacter baumannii ATCC 19606 and Bacillus subtilis ATCC 6633. The GC-MS analysis of extracts were performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). The compounds were identified by comparing the mass spectrum ratio of the sample with the data available in NIST 2014 Mass Spectral Library.

Result and Discussion: As a result, it was found that the branch extracts were more effective than the leaf extracts and both branch and leaf extracts showed the highest activity against Bacillus subtilis ATCC 6633 strain (48.8 µg/ml, 97.6 µg/ml, respectively). The extracts also showed antibacterial activity at varying concentrations on other test strains.

Anahtar Kelimeler

• Antibacterial
• branch
• GC-MS
• leaf
• Quercus macranthera subsp. syspirensis

Kaynakça

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