KURUTMA YÖNTEMİNE GÖRE NANE BİTKİSİNİN FENOLİK İÇERİĞİNİN VE ANTİOKSİDAN AKTİVİTESİNİN KARŞILAŞTIRILMASI

Öz

Amaç: Bitkilerin antioksidan aktiviteleri kurutma yöntemlerinden önemli ölçüde etkilenir. Bu çalışmada, hava ile kurutulmuş (HK) ve dondurularak kurutulmuş (DK) nanenin (Mentha piperita L.) sulu ekstrelerinde toplam fenolik içeriği ve antioksidan aktivitesi ölçülmüştür. Amaç, kurutma yöntemine göre antioksidan aktivitesinde değişiklik olup olmadığını belirlemektir.

Gereç ve Yöntem: Bu bitkilerin toplam fenolik bileşiklerini ve antioksidan kapasitelerini belirlemek için Folin-Ciocalteu, CUPRAC, DPPH, ABTS, DMPD yöntemleri kullanılmıştır.

Sonuç ve Tartışma: Sonuçlar, gallik asit, trolox, askorbik asit ve butile hidroksianisol (BHA) gibi bilinen antioksidan standartlarla karşılaştırılarak değerlendirilmiştir. Total fenolik madde içeriği (TFM), hava ile kurutulmuş bitki ekstresi için 59.91 mg/g GAE (Gallik asit eşdeğeri), liyofilize bitki ekstresi için 58.2 mg/g GAE olarak hesaplanmıştır. Tüm yöntemlerden elde edilen sonuçlara göre, HK örneklerinde radikal süpürme aktivitesinin DK örneklerinden biraz daha yüksek olduğu bulunmuştur. Sonuç olarak, havayla kurutmanın, nanenin antioksidan aktivitesine katkıda bulunan bileşiklerde bozulmaya neden olmadığı anlaşılmıştır.

Anahtar Kelimeler

• Hava ile kurutma
• antioksidan aktivite
• dondurarak kurutma
• nane

Proje Numarası

02/2012-47

COMPARISON OF THE PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY IN PEPPERMINT PLANT ACCORDING TO THE DRYING METHOD

Öz

Objective: Antioxidant activities of the plants were significantly affected by their drying methods. In the current study, total phenolic contents and antioxidant activity of aqueous extracts of peppermint (Mentha piperita L.) dried by two different methods, air-dried (AD) and freeze-dried (FD) were measured. The aim was to determine if there were any changes in the antioxidant activity according to the drying method.

Material and Method: In order to determine the total phenolic compounds and antioxidant capacity of air-dried (AD) and freeze-dried (FD) mint, were used Folin Ciocalteu, CUPRAC, DPPH, ABTS, DMPD methods.

Result and Discussion: Results were evaluated by comparing with the known antioxidant standards, such as gallic acid, trolox, ascorbic acid, and butylatedhydroxyanisole (BHA). The total phenolic contents (TPC) were calculated as 59.9 mg/g GAE for AD plant extract and 58.2 mg/g GAE for FD extract. According to the obtained results from all methods, radical scavenging activities were found to be slightly higher in AD samples than in FD samples. In conclusion, the results show that air-drying does not cause degradation in compounds that contribute to the antioxidant activity of the peppermint. 

Anahtar Kelimeler

• Air-drying
• antioxidant activity
• freeze-drying
• peppermint

Destekleyen Kurum

Gazi University Research Fund

Proje Numarası

02/2012-47

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