NASAL CARRIAGE OF STAPHYLOCOCCUS AUREUS IN PHARMACIST AND PHARMACY PERSONNEL

Öz

Objective: The aim of this study was to determine the Staphylococcus aureus (S. aureus) nasal carriage rates and risk factors in pharmacist and pharmacy personnel.

Material and Method: 300 nasal swabs were collected from volunteers (pharmacist and pharmacy personnel) working in pharmacies in Ankara, Turkey. Samples were identified as S. aureus by phenotypic methods. Methicillin resistance of the strains was determined in accordance with the recommendations of the Clinical and Laboratory Standards Institute (CLSI) by the disk diffusion method and the presence of the mecA gene was investigated by Polymerase Chain Reaction (PCR). Volunteers were asked to answer some questions (age, sex etc.) and risk factors for nasal S. aureus carriage were investigated.

Result and Discussion: S. aureus was detected in 64 (21.3%) of 300 samples and of which 4 (1.3%) were identified as Methicillin Resistance Staphylococcus aureus (MRSA). S. aureus carriage rates were found to be 25.7% in pharmacist and 20% in pharmacy personnel. There was no significant difference between these two groups (p>0.05). A significant difference was found between some risk factors (smoking, diabetes, and outpatient treatment in hospital within the past year) and nasal S. aureus carriage (p<0.05). We think that compliance with hand hygiene and effective infection control policies can reduce the rates of S. aureus and MRSA carriage.

Anahtar Kelimeler

• mecA
• methicillin resistance Staphylococcus aureus
• polymerase chain reaction
• Staphylococcus aureus

ECZACI VE ECZANE PERSONELİNDE STAPHYLOCOCCUS AUREUS BURUN TAŞIYICILIĞI

Öz

Amaç: Bu çalışmanın amacı eczacı ve eczane personelindeki Staphylococcus aureus (S. aureus) burun taşıyıcılık oranlarının ve risk faktörlerinin belirlenmesidir.

Gereç ve Yöntem: Ankara’daki eczanelerde çalışan eczacı ve eczane personelinden oluşan gönüllü bireylerden 300 nazal sürüntü örneği toplandı. Örnekler fenotipik yöntemler ile S. aureus olarak tanımlandı. Suşların metisilin direnci CLSI önerileri doğrultusunda disk difüzyon yöntemi ile belirlendi ve mecA geninin varlığı polimeraz zincir reaksiyonu (PZR) ile araştırıldı. Gönüllülerden bazı sorulara (yaş, cinsiyet vb.) cevap vermesi istenerek nazal S. aureus taşıyıcılığı için risk faktörleri araştırıldı.

Sonuç ve Tartışma: 300 örneğin 64’ü (21.3%) S. aureus ve 4’ü (1.3%) metisilin dirençli Staphylococcus aureus (MRSA) olarak tespit edildi. Nazal S. aureus taşıyıcılık oranları eczacılarda % 25.7, eczane personelinde % 20 olarak belirlendi. Bu iki grup arasında anlamlı bir fark bulunmadı (p>0.05). Bazı risk faktörleri (sigara içmek, şeker hastası olmak ve son bir yıl içerisinde hastanede ayakta tedavi görmek) ile nazal S. aureus taşıyıcılığı arasında anlamlı bir fark bulundu (p<0.05). El hijyenine ve enfeksiyon kontrol politikalarına uyumun S. aureus ve MRSA taşıyıcılık oranlarını azaltabileceğini düşünmekteyiz.

Anahtar Kelimeler

• mecA
• methicillin resistance Staphylococcus aureus
• polymerase chain reaction
• Staphylococcus aureus

Kaynakça

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