EXTRACTION-FREE SPECTROPHOTOMETRIC DETERMINATION OF MELOXICAM USING BROMOTHYMOL BLUE

Öz

Objective: The purpose of the present work was to develop and validate a fast, simple and sensitive extraction-free spectrophotometric technique for the quantitative determination of meloxicam based on the reaction with bromothymol blue. Material and Method: The reference standard of meloxicam, bromothymol blue and finished dosage forms of meloxicam were used in the study. Absorption measurements were performed on the Analytic Jena UV-visible spectrophotometer model Specord 200. Result and Discussion: The developed method is based on the formation of the colored reaction product between meloxicam and bromothymol blue in acetone medium with absorption maximum at 348 nm. The method meets the requirements of the State Pharmacopoeia of Ukraine for such validation characteristics as specificity, linearity, precision, accuracy, robustness and range of application. The obedience to Beer's law is observed in the range of meloxicam concentrations 0.80-2.40 mg/100 ml, the correlation coefficient is 0.9998. The range of application of the method is 60-140%.

Anahtar Kelimeler

• Meloxicam
• bromothymol blue
• sulfonephthalein dyes
• spectrophotometry
• validation studies

Destekleyen Kurum

Zaporizhzhia State Medical University

BROMOTİMOL MAVİSİ KULLANILARAK EKSTRAKSİYON OLMADAN MELOKSİKAMIN SPEKTROFOTOMETRİK TAYİNİ

Öz

Amaç: Mevcut çalışmanın amacı, bromotimol mavisi ile reaksiyona dayalı olarak meloksikamın kantitatif tayini için hızlı, basit ve hassas ekstraksiyon gerektirmeyen bir spektrofotometrik teknik geliştirmek ve doğrulamaktır. Gereç ve Yöntem: Çalışmada meloksikamın referans standardı, bromotimol mavisi ve meloksikamın bitmiş dozaj formları kullanılmıştır. Absorpsiyon ölçümleri, Analitik Jena UV-görünür spektrofotometre modeli Specord 200 üzerinde gerçekleştirilmiştir. Sonuç ve Tartışma: Geliştirilen yöntem, 348 nm’de maksimum absorpsiyon gözlenerek aseton ortamında meloksikam ve bromotimol mavisi arasındaki reaksiyonun boyalı ürününün oluşmasına dayanmaktadır. Geliştirilen teknik, özgüllük, doğrusallık, kesinlik, doğruluk, sağlamlık ve uygulama aralığı gibi validasyon özellikleri için Ukrayna Devlet Farmakopesi şartlarını karşılamaktadır. Beer yasasına uygunluk 0.80-2.40 mg/100 ml konsantrasyon aralığında gözlemlenir. Korelasyon katsayısı 0.9998’dir. Tekniğin uygulama aralığı %60-140’tır.

Anahtar Kelimeler

• Meloksikam
• bromotimol mavisi
• sülfonatalein boyaları
• spektrofotometri
• validasyon çalışmaları

Kaynakça

1. 1. Ghlichloo, I., Gerriets, V. (2021). Nonsteroidal anti-inflammatory drugs (NSAIDs). StatPearls. Retrieved May 2, 2022, from https://www.statpearls.com/articlelibrary/viewarticle/25968/.
2. 2. Buheruk, V.V., Voloshyna, O.B., Kovalchuk, L.I., Balashova, I.V., Naidionova, O.V. (2021). Potential role of acetylsalicylic acid and other non-steroidal anti-inflammatory drugs in cancer risk reduction (literature review). Zaporozhye Medical Journal, 23(3), 436-441. [CrossRef]
3. 3. Wongrakpanich, S., Wongrakpanich, A., Melhado, K., Rangaswami, J. (2018). A comprehensive review of non-steroidal anti-inflammatory drug use in the elderly. Aging and Disease, 9(1), 143. [CrossRef]
4. 4. Khalil, N.Y., Aldosari, K.F. (2020). Meloxicam. Profiles of Drug Substances, Excipients and Related Methodology, 45, 159-197. [CrossRef]
5. 5. Engelhardt, G., Homma, D., Schlegel, K., Utzmann, R., Schnitzler, C. (1995). Anti-inflammatory, analgesic, antipyretic and related properties of meloxicam, a new non-steroidal anti-inflammatory agent with favourable gastrointestinal tolerance. Inflammation Research, 44(10), 423-433. [CrossRef]
6. 6. Council of Europe. (2016). Meloxicam. In European pharmacopoeia: Publ. in accordance with the convention on the elaboration of a European pharmacopoeia, pp. 2994-2996.
7. 7. United States Pharmacopoeial Convention. (2019). Meloxicam. In United States pharmacopoeia: The National Formulary, pp. 2767-2769.
8. 8. Mahood, A.M., Najm, N.H. (2019). Spectrophotometric estamation of meloxicam using charge transfer complex. IOP Conference Series: Materials Science and Engineering, 571(1), 012081. [CrossRef]

9. 9. Abbas, R.F., Mahdi, N.I., Amer Waheb, A.A., Aliwi, A.G., Falih, M.S. (2019). Fourth derivative and compensated area under the curve spectrophotometric methods used for analysis meloxicam in the local market tablet. Al-Mustansiriyah Journal of Science, 29(3), 70-76. [CrossRef]
10. 10. Hasan, S.H., Othman, N.S., Surkhi, K.M. (2014). Spectrophotometric method for determination of meloxicam in pharmaceutical formulations using N-bromosuccinimide as an oxidant. International Journal of Pharma Sciences and Research, 5(12), 963-969.
11. 11. Gumułka, P., Dąbrowska, M., Starek, M. (2020). Microanalysis of selected NSAIDs using the spectrophotometric method. Eng, 1(2), 211-221. [CrossRef]
12. 12. Mallikarjuna, H., Shivaprasad, K.H., Venugopala Reddy, K.R., Lokesh K.S. (2016). Spectrophotometric determination of some non-steroidal anti-inflammatory drugs by oxidative coupling reaction. Asian Journal of Research in Chemistry, 3(3), 1070.
13. 13. Ahmad, S., Deepika, S., Amol, P., Kapil, W., Md. Usman, M.R. (2017). Novel RP-HPLC method development and validation of meloxicam suppository. Indian Journal of Pharmaceutical Education and Research, 51(4), 644-649. [CrossRef]
14. 14. Jallal, F.A., Baban, O.S. (2011). Determination of meloxicam in pharmaceutical formulation Byazo-coupling reaction with sulphanilic acid using both batch and flow-injection technique. Rafidain Journal of Science, 22(7), 121-132. [CrossRef]
15. 15. Sahoo, N.K., Sahu, M., Rao, P.S., Rani, N.S., Devi, J.N.V.I., Ghosh, G. (2014). Validation of assay indicating method development of meloxicam in bulk and some of its tablet dosage forms by RP-HPLC. SpringerPlus, 3(1), 94. [CrossRef]
16. 16. Cox, S., Hayes, J., Yarbrough, J., Veiga-Parga, T., Greenacre, C. (2014). High-performance liquid chromatography determination of meloxicam and piroxicam with ultraviolet detection. Chromatography Research International, 2014, 521697. [CrossRef]
17. 17. Parys, W., Bober, K., Pyka-Pająk, A., Dołowy, M. (2019). The application of TLC and densitometry for quantitative determination of meloxicam in tablets. Current Pharmaceutical Analysis, 15(7), 785-794. [CrossRef]
18. 18. Cerón-Pérez, A., Juárez-Moreno, M.G., Martínez-Sánchez, M.M., González-Leal, M., Sosa-Domínguez, A. (2021). Developing a voltammetric method for meloxicam determination using a glassy carbon electrode modified with multi-walled carbon nanotubes (GC/MWCNT). ECS Transactions, 101(1), 57-67. [CrossRef]
19. 19. Chaplenko, A.A., Monogarova, O.V., Oskolok, K.V. (2018). Spectroscopic and colorimetric determination of meloxicam, lornoxicam, tenoxicam in drugs. International Journal of Pharmaceutical and Biological Archives, 9(1), 31-35.
20. 20. Abed, R.I., Hadi, H. (2021). Determination of meloxicam using direct and indirect flow injection spectrophotometry. Current Pharmaceutical Analysis, 17(2), 254-264. [CrossRef]
21. 21. Sane, R.T., Surve, V., Francis, M. (2000). Extractive colorimetric determination of meloxicam from its pharmaceutical preparation. Indian Drugs, 8, 390-393.
22. 22. Bishop, E. (1972). Indicators. International series of monographs in analytical chemistry. Pergamon Press, Oxford, p. 756.
23. 23. Florea, M., Ilie, M. (2017). Ion-pair spectrophotometry in pharmaceutical and biomedical Analysis: Challenges and perspectives. Spectroscopic Analyses - Developments and Applications. [CrossRef]
24. 24. Alizadeh, N., Rezakhani, Z. (2012). Extractive spectrophotometric determination of ketoconazole, clotrimazole and fluconazole by ion-pair complex formation with bromothymol blue and picric acid. Journal of the Chilean Chemical Society, 57(2), 1104-1108. [CrossRef]
25. 25. Nguyen, T.D., Le, H.B., Dong, T.O., Pham, T.D. (2018). Determination of fluoroquinolones in pharmaceutical formulations by extractive spectrophotometric methods using ion-pair complex formation with bromothymol blue. Journal of Analytical Methods in Chemistry, 2018, 1-11. [CrossRef]
26. 26. Rahman, N., Manirul Haque, S., Azmi, S.N.H., Rahman, H. (2017). Optimized and validated spectrophotometric methods for the determination of amiodarone hydrochloride in commercial dosage forms using N-bromosuccinimide and bromothymol blue. Journal of Saudi Chemical Society, 21(1), 25-34. [CrossRef]
27. 27. Nguyen, T.D. (2022). Extractive spectrophotometric determination of nimodipine through ion-pair complex formation with bromothymol blue. Journal of Science and Technique, 17(1). [CrossRef]
28. 28. Prashanth, K.N., Basavaiah, K., Raghu, M.S. (2012). Rapid and sensitive spectrophotometric measurement of nonspecific beta blocker propranolol hydrochloride using three sulphonphthalein dyes in pure form, pharmaceuticals and human urine. Chemical Sciences Journal, 2012, 1-14.
29. 29. State Pharmacopoeia of Ukraine (2015). (2nd ed). Kharkiv: Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines, p.1148.