IN VITRO ASSESSMENT OF HEMOSTATIC PERFORMANCES OF SALVIA VERTICILLATA L, ACHILLEA BIEBERSTEINII AFAN., TRAGOPOGON AUREUS BOISS., AND CEPHALARIA PROCERA FISCH. & AVÉ-LALL.

Yıl 2023, , 883 - 893, 20.09.2023

Songül Karakaya , Özlem Özdemir , Ümit İncekara , Hasan Türkez , Oksana Sytar , Özkan Aksakal

https://doi.org/10.33483/jfpau.1266421

Cited By: 1

Öz

Objective: Hemostasis is an inherent function and natural process to prevent or stop bleeding. Nowadays great efforts are being made to develop novel, economical and high-performance products to control bleeding. In this study, we aimed to assess the in vitro hemostatic effects of four several plant species used in folk medicine for different purposes including Salvia verticillata, Achillea biebersteinii, Tragopogon aureus, and Cephalaria procera.
Material and Method: The extracts with different solvent nature were prepared and their hemostatic efficacy were determined using optical aggregometry.
Result and Discussion: The present results clearly revealed that the extracts of S. verticillata showed the highest efficacy on platelet aggregation in presence of adenosine-diphosphate (ADP) (80.77%), collagen (80.78%), and arachidonic acid (AA) (73.71%) when compared to other plant extracts. Again, the most effective platelet aggregation (47.27%) was determined after the application C. procera within the presence of epinephrine (EPI). Moreover, we first executed that ethyl acetate extracts led to the highest percentages of platelet aggregation in the fact of APD, collagen, AA and EPI. In a conclusion, our findings suggested that the tested medicinal plants in particular S. verticillata and C. procera could be novel and natural sources of effective hemostatic agents.

Anahtar Kelimeler

Achillea biebersteinii, Cephalaria procera, hemostasis, Salvia verticillata, Tragopogon aureus

Destekleyen Kurum

Atatürk University (FBA-2018-6832).

Proje Numarası

FBA-2018-6832

SALVIA VERTICILLATA L., ACHILLEA BIEBERSTEINII AFAN., TRAGOPOGON AUREUS BOISS. VE CEPHALARIA PROCERA FISCH. & AVÉ-LALL.’NIN HEMOSTATİK PERFORMANSLARININ İN VİTRO DEĞERLENDİRİLMESİ

Öz

Amaç: Hemostaz, kanamayı önlemek veya durdurmak için doğal bir işlev ve doğal bir süreçtir. Günümüzde kanama kontrolü için yeni, ekonomik ve yüksek performanslı ürünler geliştirmek için büyük çaba sarf edilmektedir. Bu çalışmada, halk hekimliğinde farklı amaçlarla kullanılan Salvia verticillata, Achillea biebersteinii, Tragopogon aureus ve Cephalaria procera gibi dört farklı bitki türünün in vitro hemostatik etkilerini değerlendirmeyi amaçladık.
Gereç ve Yöntem: Farklı polaritelerde ekstreler hazırlandı ve hemostatik etkinlikleri optik agregometri kullanılarak belirlendi.
Sonuç ve Tartışma: Mevcut sonuçlar, diğer bitki özleri ile karşılaştırıldığında S. verticillata ekstrelerinin adenozin-difosfat (ADP) (%80.77), kollajen (%80.78) ve araşidonik asit (AA) (%73.71) varlığında trombosit agregasyonu üzerinde en yüksek etkinliği gösterdiğini açıkça ortaya koymuştur. Yine epinefrin (EPI) varlığında en etkili trombosit agregasyonu (%47.27) C. procera uygulamasından sonra belirlendi. Ayrıca, öncelikle etil asetat ekstrelerinin APD, kollajen, AA ve EPI olgusunda en yüksek trombosit agregasyonu yüzdelerini göstermiştir. Sonuç olarak, bulgularımız, özellikle S. verticillata ve C. procera'nın etkili hemostatik ajanların yeni ve doğal kaynakları olabileceğini gösterdi.

Anahtar Kelimeler

Achillea biebersteinii, Cephalaria procera, hemostaz, Salvia verticillata, Tragopogon aureus

Proje Numarası

FBA-2018-6832

Kaynakça

• 1. Nayal, R., Abajy, M.Y., Takla, S. (2015). Investigating in vitro the hemostatic effect of some medicinal plants, Res. J of Aleppo univ, Medicine Sience Series, No.100.
• 2. Yılmaz, Y.K. (2013). Vascular injuries: Introduction, history, the diagnosis and treatment methods. Turkiye Klinikleri Cardiovascular Surgery-Special Topics, 5(2), 18-25.
• 3. Periayah, M.H., Halim, A.S., Mat, S.A.Z. (2017). Mechanism action of platelets and crucial blood coagulation pathways in hemostasis. International Journal of Hematology-Oncology and Stem Cell Research, 11(4), 319-327.
• 4. Ghoshal, K., Bhattacharyya, M. (2014). Overview of platelet physiology: Its hemostatic and nonhemostatic role in disease pathogenesis. The Scientific World Journal, 2014, 781857. [CrossRef]
• 5. Paniccia, R., Priora, R., Liotta, A.A., Abbate, R. (2015). Platelet function tests: A comparative review. Vascular Health and Risk Management, 11, 133-148. [CrossRef]
• 6. Ebrahimia, F., Torbati, M., Mahmoudic, J., Valizadeh, H. (2020). Medicinal plants as potential hemostatic agents. Journal of Pharmacy & Pharmaceutical Science, 23, 11-23. [CrossRef]
• 7. Born, G.V.R. (1962). Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature, 194, 927-929. [CrossRef]
• 8. Zhou, L., Schmaier, A.H. (2005). Platelet aggregation testing in platelet-rich plasma: Description of procedures with the aim to develop standards in the field. American Journal of Clinical Pathology, 123, 172-183. [CrossRef]
• 9. Yarali, N., Oruc, M., Bay, A., Dalgic, B., Bozkaya, I.O., Arıkoglu, T., Kara, A., Bahattin, T. (2010). A new hemostatic agent-Ankaferd blood stopper: Management of gastrointestinal bleeding in an infant and other experiences in children. Pediatric Hematology and Oncology, 27, 592-596. [CrossRef]
• 10. Okten, S., Kurt, M., Onal, I.K, Haznedaroglu, I.C. (2011). Use of Ankaferd blood stopper for controlling actively bleeding fundal varices. Singapore Medical Journal, 52(1), e11-2.
• 11. Bilgili, H., Kosar, A., Kurt, M., Onal, I.K., Goker, H., Captug, O., Shorbagi, A., Turgut, M., Kekilli, M., Kar, K.O., Kirazli, S., Aksu, S., Haznedaroglu, I.C. (2009). Hemostatic efficacy of ankaferd blood stopper in a swine bleeding model. Medical Principles and Practice. 18, 165-169. [CrossRef]
• 12. Tepe, B., Eminagaoglu, O., Akpulat, H.A., Aydin, E. (2007). Antioxidant potentials and rosmarinic acid levels of the methanolic extracts of Salvia verticillata (L.) subsp. verticillata and S. verticillata (L.) subsp. amasiaca (Freyn & Bornm.) Bornm. Food Chemistry, 100(3), 985-989. [CrossRef]
• 13. Matkowski, A., Zielińska, S., Oszmiański, J., Lamer-Zarawsk, E. (2008). Antioxidant activity of extracts from leaves and roots of Salvia miltiorrhiza Bunge, S. przewalskii Maxim., and S. verticillata L. Bioresource Technology, 99(16), 7892-7896. [CrossRef]
• 14. Jaffal, S.M., Manal, A.A. (2019). Antinociceptive action of Achillea biebersteinii methanolic fower extract is mediated by interaction with cholinergic receptor in mouse pain models. Infammopharmacology, 27, 961-968. [CrossRef]
• 15. Katanić, S.S., Srećković, N., Mišić, D., Gašić, U., Imbimbo, P., Monti, D.M., Mihailović, V. (2020). Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae)-A plant rich in rosmarinic acid. Industrial Crops and Products, 143, 111932. [CrossRef]
• 16. Plantlist Web site. (2019). Retrieved August 23, 2019, from http://www.theplantlist.org/tpl1.1/record/gcc-131516. Erişim tarihi: 23.08.2019.
• 17. Coruh, I., Gormez, A.A., Ercisli, S., Bilen, S. (2007). Total phenolics, mineral elements, antioxidant and antibacterial activities of some edible wild plants in Turkey. Asian Journal of Chemistry, 19(7), 5755-5762.
• 18. Karakaya, S., Polat, A., Aksakal, Ö., Sümbüllü, Y.Z., İncekara, Ü. (2019). An ethnobotanical investigation on medicinal plants in South of Erzurum (Turkey). Ethnobotany Research & Applications, 18, 13.
• 19. Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler), İstanbul: Nezahat Gökyiğit Botanic Garden Publications, p.108, 211, 316, 580.
• 20. Ozgen, U., Kaya, Y., Coskun, M. (2004). Ethnobotancal studies in the villages of the district of Ilıca (province Erzurum), Turkey. Economic Botany, 58(4), 691-696.
• 21. Tozlu, G. (2010). Biological observations on Agapanthia osmanlis Reiche & Saulcy (Coleoptera: Cerambycidae) associated with Cephalaria procera Fisch & Lall. (Dipsacaceae) in Northeastern Turkey. Journal of Entomological Research Society, 12(1), 15.
• 22. Plantlist Web site. (2019). Retrieved August 23, 2019, from http://www.theplantlist.org/tpl1.1/record/kew-2709407. Erişim tarihi: 23.08.2019.
• 23. Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 6, 144-158.
• 24. Slinkard, K., Singleton, V.L. (1977). Total phenols analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28, 49-55.
• 25. Mavi, A., Terzi, Z., Ozgen, U., Yildirim, A., Coşkun, M. (2004). Antioxidant properties of some medicinal plants: Prangos ferulacea (Apiaceae), Sedum sempervivoides (Crassulaceae), Malva neglecta (Malvaceae), Cruciata taurica (Rubiaceae), Rosa pimpinellifolia (Rosaceae), Galium verum subsp. verum (Rubiaceae), Urtica dioica (Urticaceae). Biological and Pharmaceutical Bulletin, 27, 702-705. [CrossRef]
• 26. Brand, W.W., Cuvelier, M.E., Berset, C. (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28, 25-30. [CrossRef]
• 27. Ugur, A., Saraç, N., Çankal, D.A., Özle, M. (2016). The antioxidant and antimutagenic activities of Ankaferd blood stopper, a natural hemostatic agent used in dentistry. Turkish Journal of Medical Sciences, 46, 657-663. [CrossRef]
• 28. Páez, X., Hernández, L. (2003). Topical hemostatic effect of a common ornamental plant, the Geraniaceae Pelargonium zonale. The Journal of Clinical Pharmacology, 43, 291-295. [CrossRef]
• 29. Kose, R., Sogut, O., Erdemir, T., Koruk, I. (2012). Hemostatic efficacy of folkloric medicinal plant extract in a rat skin bleeding model. Dermatologic Surgery, 38, 760-766. [CrossRef]
• 30. Mekhfi, H., El Haouari, M., Legssyer, A., Bnouham, M., Aziz, M., Atmani, F., Adnane, R., Abderrahim, Z. (2004). Platelet anti-aggregant property of some Moroccan medicinal plants. Journal of Ethnopharmacology, 94, 317-322. [CrossRef]