ASKORBİK ASİT İÇEREN POLİELEKTROLİT KİTOZAN-JELATİN HİDROJELLERİN GELİŞTİRİLMESİ VE KARAKTERİZASYONU

Öz

Amaç: Çalışmanın amacı, güçlü bir antioksidan olan askorbik asit içeren, polielektrolit kompleksi olan ve olmayan kitozan-jelatin hidrojelleri formüle etmektir. Gereç ve Yöntem: Polielektrolit kompleksi oluşumunun, jelatin konsantrasyonunun (%10-20) ve kitozan:jelatin oranının (1:1, 1:2, 2:1 a/a) reolojik özellikler, in vitro salım ve enkapsülasyon etkinliği üzerindeki etkisi araştırılmıştır. Salım sonuçları AUC, MDT ve MRT kullanılarak karşılaştırılmıştır. Ayrıca MTT testi kullanılarak L929 hücre hattı üzerindeki 24 ve 72 saatlik sitotoksik ve proliferatif etkileri incelenerek geliştirilen hidrojelin topikal kullanım potansiyeli değerlendirilmiştir Sonuç ve Tartışma: Polielektrolit kompleksi oluşumu, ilaç salımının gelişmesine ve viskozitenin artmasına yol açmıştır. Boş ve ilaç yüklü polielektrolit hidrojellerin hücre canlılığının 72 saat sonunda tüm formülasyonlarda (kitozan:jelatin oranı 1:2 a/a olan formülasyonlar hariç) %70’in üzerinde olması, askorbik asit ve hidrojellerin hücresel toksisiteye neden olmadığını ve güvenle kullanılabilir olduğunu göstermektedir. Jelatin oranının en fazla %50 olması gerektiği ve fazla jelatinin hücre canlılığını azalttığı kanıtlanmıştır. Sonuç olarak F6 kodlu polielektrolit hidrojel (%20 jelatin; 2:1 a/a kitozan:jelatin), yüksek MDT ve AUC değerleri ve >%80 hücre canlılığı ile en uzun kontrollü ilaç salınımına yol açtığı için ideal formülasyondur. Sonuç olarak, polielektrolit kompleks oluşumu daha uygundur ve istenen özellikleri elde etmek için kitozan:jelatin oranı ve jelatin konsantrasyonu manipüle edilebilir.

Anahtar Kelimeler

• Askorbik asit (vitamin C)
• hidrojeller
• jelatin
• kitozan
• polielektrolit kompleks kitozan-jelatin

DEVELOPMENT AND CHARACTERIZATION OF ASCORBIC ACID LOADED POLYELECTROLYTE CHITOSAN-GELATIN HYDROGELS

Öz

Objective: Aim of study was to formulate chitosan-gelatin hydrogels containing ascorbic acid, an antioxidant, with/without polyelectrolyte-complex. Material and Method: Effect of formation polyelectrolyte-complex, gelatin concentration (10-20%) and chitosan:gelatin ratio(1:1, 1:2, 2:1w/w) on the rheological properties, in-vitro release, encapsulation efficiency of hydrogels were investigated. Dissolution rates were also compared using area under dissolution curve (AUC), mean dissolution time (MDT), mean residence time (MRT). Also, the potential for topical use of the hydrogel was evaluated by examining the 24-and 72-hours cytotoxic and proliferative effects on L929 cell line using MTT test. Result and Discussion: Polyelectrolyte complex formation led to improved drug release and increased viscosity. Cell viability of the free and drug-loaded polyelectrolyte-hydrogels was over 70% at the end of the 72h in all formulations (except formulations with chitosan:gelatin ratio of 1:2w/w) showed that ascorbic acid and hydrogels did not cause cellular toxicity and could be used safely. It has been demonstrated that the gelatin ratio should be at most 50%, and excess gelatin reduces cell viability. F6-coded-polyelectrolyte-hydrogel (20% gelatin; 2:1 chitosan:gelatin w/w) was ideal formulation as it led to best sustained drug release with high MDT and AUC values, and cell viability >80%. In conclusion, polyelectrolyte-complex formation is more superior, and chitosan:gelatin ratio and gelatin concentration can be manipulated to obtain the desired properties.

Anahtar Kelimeler

• Ascorbic acid (vitamin C)
• chitosan
• gelatin
• hydrogels
• polyelectrolyte complex chitosan-gelatin

Kaynakça

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