REAL-TIME MONITORING OF CYTOTOXIC ACTIVITY OF SAFRANAL COMPOUND ON LUNG CANCER AND BREAST CANCER

Öz

Objective: Lung adenocarcinoma and breast cancer are the most commonly occurring cancer types and they are the leading cause of cancer-related deaths worldwide. Safranal has various pharmacological effects and have cytotoxic properties. Aim of this study was by continuous monitoring to assay the cytotoxicity of safranal on human lung carcinoma cell line (A549), human breast cancer cell line (MCF-7) and human bronchial epithelial cell lines (Beas-2b). Material and Method: Cells were treated 1, 10, 100 µM concentrations with safranal and xCELLigence real-time cell analyzer were used to determine this compound effect on cell viability. The cell index was monitored every 15 minutes by visualizing the impedance of the E-plate wells. Result and Discussion: Consequently the studies, it was determined that the safranal has cytotoxic effect on MCF-7 cell line and did not has a toxic effect on A549 and Beas-2b cells. In the MCF-7 cell line, cell index alterations were found to be decreased at all concentrations compared with the untreated control. The combination of conventional anticancer drugs and safranal can be used to increase the effectiveness of these drugs considering cancer type.

Anahtar Kelimeler

• A549 cell line
• cytotoxicity
• MCF-7 cell line
• safranal
• xCELLigence

AKCİĞER KANSERİ VE MEME KANSERİ HÜCRELERİNDE SAFRANAL BİLEŞİĞİNİN SİTOTOKSİK AKTİVİTESİNİN GERÇEK ZAMANLI İZLENMESİ

Öz

Amaç: Akciğer adenokarsinomu ve meme kanseri en sık görülen kanser türleridir ve dünya çapında kansere bağlı ölümlerin önde gelen nedenlerindendir. Safranal bileşiğinin çeşitli farmakolojik etkileri ve sitotoksik özellikleri vardır. Bu çalışmanın amacı, safranal bileşiğinin insan akciğer karsinomu hücre hattı (A549), insan meme kanseri hücre hattı (MCF-7) ve insan bronşiyal epitel sağlıklı hücre (Beas-2b) hatları üzerindeki sitotoksisitesini sürekli izleme yoluyla tahlil etmektir. Gereç ve Yöntem: Hücreler, safranal ile 1, 10, 100 µM konsantrasyonda muamele edildi ve bu bileşiğin hücre canlılığı üzerindeki etkisini belirlemek için xCELLigence gerçek zamanlı hücre analizörü kullanılmıştır. E-plaka kuyularının empedansı aracılığıyla hücre indeksi görüntülenerek her 15 dakikada bir izlenmiştir. Sonuç ve Tartışma: Yapılan çalışmalar sonucunda safranal bileşiğinin MCF-7 hücre hattı üzerinde sitotoksik etkiye sahip olduğu, A549 ve Beas-2b hücreleri üzerinde toksik etkisinin olmadığı belirlenmiştir. MCF-7 hücre hattında, hücre indeksi değişiklikleri, kontrol grubu ile karşılaştırıldığında tüm konsantrasyonlarda azaldığı tespit edilmiştir. Kanser türüne göre bu ilaçların etkinliğini artırmak için geleneksel antikanser ilaçlar ve safranal bileşiği kombinasyonu kullanılabilir.

Anahtar Kelimeler

• A549 hücre hattı
• MCF-7 hücre hattı
• safranal
• sitotoksisite
• xCELLigence

Kaynakça

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