AMFOTERİSİN-B ENKAPSÜLE EDİLMİŞ NANOPARTİKÜLLERİN ANTİMİKROBİYAL POTENSİNİN DEĞERLENDİRİLMESİ

Öz

Amaç: Bu çalışmanın amacı, Amfoterisin B yüklü polimerik nanopartiküllerin geliştirilmesi ve Amfoterisin B nanoformülasyon örneklerinin ve ticari olarak temin edilen Amfoterisin B örneklerinin potensinin, Amerika Birleşik Devletleri Farmakopesi'nde ayrıntılı olarak açıklanan protokole göre referans Amfoterisin B standardı ile karşılaştırmalı olarak belirlenmesidir. Gereç ve Yöntem: Amfoterisin B nanopartikülleri tek emülsiyon yöntemi kullanılarak üretilmiştir. Referans Amfoterisin B standardının antimikrobiyal potensi ile ticari Amfoterisin B ve Amfoterisin B'nin nanoformülasyonunun potenslerinin kıyaslanması, Amerika Birleşik Devletleri Farmakopesinde belirtilen disk difüzyon yöntemi kullanılarak gerçekleştirilmiştir. Sonuç ve Tartışma: Ortalama hidrodinamik çapı 215.14±0.72 nm ve PDI değeri 0.18±0.02 olan Amfoterisin B yüklü poli(etilen glikol)-b-poli(ɛ-kaprolakton) nanopartikülleri başarıyla geliştirilmiştir. HPLC yöntemi kullanılarak belirlenen Amfoterisin B enkapsülasyon etkinliği %66.4±1.42 olarak bulunmuştur. Ticari Amfoterisin B'nin % potensi %95.7 olarak hesaplanırken, Amfoterisin B'nin nanoformülasyonunun % potensi %99.1 olarak hesaplanmış olup bu bulgu, taşıyıcı sistem olarak polimerik nanopartiküllerin kullanılmasının avantajını ortaya koymaktadır.

Anahtar Kelimeler

• Amfoterisin B
• antimikrobiyal potens
• farmakope yöntemi
• polimerik nanopartiküller

DEVELOPMENT OF AMPHOTERICIN-B LOADED NANOPARTICLES AND EVALUATION THE ANTIMICROBIAL POTENCY

Öz

Objective: The aim of this study is the development of Amphotericin B loaded polymeric nanoparticles and the determination of the potency of Amphotericin B nanoformulation samples and commercially supplied Amphotericin B samples in comparison with reference Amphotericin B standard, according to the protocol detailed in the United States Pharmacopoeia. Material and Method: Amphotericin B nanoparticles were fabricated using single emulsion method. The comparison of the potencies of the AmB nanoformulation and commercial Amphotericin B with the antimicrobial potency of the reference Amphotericin B standard was performed using the disk diffusion method specified in the United States Pharmacopeia. Result and Discussion: Amphotericin B loaded poly(ethylene glycol)-b-poly(ɛ-caprolactone) nanoparticles successfully developed having the average hydrodynamic diameter of 215.14±0.72 nm and PDI value of 0.18±0.02. The Amphotericin B encapsulation efficiency, which was determined using an HPLC method, was 66.4±1.42%. The % potency of commercial Amphotericin B was calculated as 95.7%, while the % potency of the nanoformulation of Amphotericin B was calculated as 99.1%, indicating the favor of utilizing polymeric nanoparticles as delivery systems.

Anahtar Kelimeler

• Amphotericin B
• antimicrobial potency
• pharmacopoeia method
• polymeric nanoparticles

Kaynakça

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