INVESTIGATION OF THE EFFECT OF WHEAT GERM OIL ON DIABETES-RELATED TESTICULAR DAMAGE IN TERMS OF OXIDATIVE STRESS PARAMETERS

Yıl 2024, , 158 - 168, 20.01.2024

Aylin Balcı Özyurt , Sezen Yılmaz Sarıaltın

https://doi.org/10.33483/jfpau.1384461

Öz

Objective: Our study aimed to investigate the oxidative stress-mediated effect of wheat germ oil (WGO) on testicular damage caused by diabetes.
Material and Method: 42 male Wistar albino rats were randomly divided into 6 groups: control, control low-dose (100 mg/kg/day), control high-dose (1000 mg/kg/day), diabetes control, diabetes low-dose (100 mg/kg/day), diabetic high-dose (1000 mg/kg/day). RY/carrier cornoil was applied to the diabetes and control groups by gavage for 28 days. Blood glucose levels were measured every week. Following euthanasia, testicular tissues were removed. Tissues were homogenized and total protein levels were measured by Bradford method. MDA levels were measured in tissues as an indicator of lipid peroxidation, and glutathione levels were measured as an indicator of oxidative stress.
Result and Discussion: Both low and high dose WGO administration in the control group caused a decrease in relative testicular weight. The relative testicular weight of the high dose diabetic group decreased compared to the diabetic control group. There was no significant difference in blood glucose levels and body weights depending on WGO (p>0.05). Among diabetic animals, the lowest testicular MDA levels were found in the high -dose group. Glutathione levels increased in the control low-dose group. However, there was no significant difference between the groups in terms of MDA and glutathione levels (p>0.05). Further research is needed to investigate the effectiveness of WGO, which has a traditionally usage and antioxidant activity, on sexual hormones and other molecular pathways.

Anahtar Kelimeler

Germ oil, oxidative stress, testicular damage, wheat germ

Proje Numarası

21B0237006

RUŞEYM YAĞININ DİYABETE BAĞLI TESTİKÜLER HASAR ÜZERİNDEKİ ETKİSİNİN OKSİDATİF STRES PARAMETRELERİ YÖNÜNDEN İNCELENMESİ

Öz

Amaç: Ruşeym yağının (RY) diyabetin neden olduğu testiküler hasar üzerinde oksidatif stres aracılı etkisinin araştırılması amaçlanmıştır.
Gereç ve Yöntem: 42 erkek Wistar albino sıçan randomize 6 gruba ayrılmıştır: kontrol, kontrol düşük doz (100 mg/kg/gün), kontrol yüksek doz (1000 mg/kg/gün), diyabet kontrol, diyabet düşük doz (100 mg/kg/gün), diyabet yüksek doz (1000 mg/kg/gün). Diyabet ve kontrol gruplarına 28 gün süre ile gavajla RY uygulanmıştır. Her hafta kan glukoz düzeyleri ölçülmüştür. Ötenazinin ardından testis dokuları çıkartılmıştır. Dokular homojenize edilmiş ve Bradford yöntemi ile total protein düzeyleri ölçülmüştür. Lipit peroksidasyonunun göstergesi olarak dokularda MDA düzeyleri, oksidatif stresin göstergesi olarak glutatyon düzeyleri ölçülmüştür.
Sonuç ve Tartışma: Kontrol grubunda hem düşük, hem de yüksek doz RY uygulaması rölatif testis ağırlığında azalmaya neden olmuştur. Diyabetik yüksek doz RY grubunun rölatif testis ağırlığı diyabet kontrol grubuna göre azalmıştır. Kan glukoz düzeyleri ve vücut ağırlıklarında RY’ye bağlı olarak anlamlı farklılık görülmemiştir (p>0.05). Diyabetik hayvanlar arasında en düşük testis MDA düzeyleri diyabet yüksek doz grubunda bulunmuştur. Kontrol düşük doz grubunda glutatyon düzeyleri artmıştır. Ancak gruplar arasında MDA ve glutatyon düzeyi bakımından anlamlı farklılık bulunmamıştır (p>0.05). Halk arasında kullanımı ve antioksidan aktivitesi olan RY’nin seksüel hormonlar ve diğer moleküler yolaklar üzerindeki etkisinin araştırılacağı yeni çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

Buğday ruşeymi, oksidatif stres, ruşeym yağı, testiküler hasar

Etik Beyan

Çalışmamız Kobay Deney Hayvanları Laboratuvarı A.Ş. Hayvan Deneyleri Yerel Etik Kurulu tarafından 15.09.2021 tarih ve 582 sayılı karar ile onaylanmıştır.

Proje Numarası

21B0237006

Teşekkür

Bu çalışma Ankara Üniversitesi Bilimsel Araştırma Projeleri tarafından desteklenmiştir (21B0237006).

Kaynakça

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