CYTOTOXICITY SCREENING AND ANTIOXIDANT CAPACITY ASSESSMENT OF THE INNER PERIANTH SEGMENTS OF 14 RUMEX SPECIES GROWN IN TÜRKİYE

Yıl 2024, , 456 - 469, 20.05.2024

Nadire Özenver , Yiğit Erkmen , Filiz Boyalı , Ömür Demirezer

https://doi.org/10.33483/jfpau.1391998

Öz

Objective: Breast cancer is one of the most prevalent cancer types worldwide. Antioxidant sources may prevent the occurrence of cancer. Natural sources rich in phenolics, thus, may provide alternate agents in the management of breast cancer. Rumex species are widely distributed in Turkish flora. Emerging evidence has pointed out the antitumoral property of Rumex species on a variety of cancer cells. In the present study, we propose to test the ethanolic extracts of the inner perianth segments of 14 Rumex species on four breast cancer cells with different origins. We also demonstrated their toxicity on healthy cells.
Material and Method: We performed the resazurin reduction assay to examine the cytotoxicity and toxicity. Furthermore, we determined the phenolic contents of the extracts as an indicator of their antioxidant profile and ascertained their antioxidant activities by DPPH radical, ABTS radical cation scavenging activity and cupric ion-reducing antioxidant capacity assays.
Result and Discussion: The ethanolic extracts of the inner perianth segments of Rumex species exhibited remarkable cytotoxicity profiles neither on breast cancer cells nor on healthy H9c2 rat myoblastoma cells. However, they usually displayed strong antioxidant activities due to possessing high phenolic content.

Anahtar Kelimeler

Antioxidant, breast cancer, cytotoxicity, rumex, total phenol

Etik Beyan

The present study does not require The Ethics Committee Approval.

Destekleyen Kurum

Hacettepe University Scientific Research Projects Unit

Proje Numarası

THD-2022-20462

Teşekkür

A part of this study was supported by grants from Hacettepe University Scientific Research Projects Unit (Project No: THD-2022-20462).

TÜRKİYE’DE YETİŞTİRİLEN 14 RUMEX TÜRÜNÜN İÇ PERİANT SEGMENTLERİNİN SİTOTOKSİSİTE TARAMA VE ANTİOKSİDAN KAPASİTE DEĞERLENDİRMESİ

Öz

Amaç: Meme kanseri dünya çapında en yaygın görülen kanser türlerinden biridir. Antioksidan kaynaklar kanserin oluşumunu önleyebilir. Dolayısıyla fenolikler açısından zengin doğal kaynaklar meme kanseri tedavisinde alternatif ajanlar sağlayabilir. Rumex türleri Türkiye florasında geniş bir dağılım göstermektedir. Bilimsel çalışmalar Rumex türlerinin çeşitli kanser hücreleri üzerindeki antitümöral özelliğine işaret etmektedir. Bu çalışmada, 14 Rumex türünün iç periant segmentlerinin etanolik ekstrelerinin farklı kökenlere sahip dört meme kanseri hücresi üzerinde test edilmeleri amaçlanmıştır. Ayrıca sağlıklı hücreler üzerindeki toksisiteleri de değerlendirilmiştir.
Gereç ve Yöntem: Sitotoksisite ve toksisiteyi incelemek için resazurin redüksiyon yöntemi kullanılmıştır. Ayrıca; antioksidan profillerinin bir göstergesi olarak ekstrelerin fenolik içerikleri belirlenmiş ve DPPH, ABTS radikali süpürücü aktivite ve bakır iyonu redükleyici antioksidan kapasite yöntemleri ile antioksidan karakteristikleri belirlenmiştir.
Sonuç ve Tartışma: Rumex türlerinin iç periant segmentlerinin etanolik ekstreleri, sağlıklı H9c2 sıçan miyoblastoma hücreleri üzerinde toksik olmamasına rağmen, meme kanseri hücreleri üzerinde dikkate değer sitotoksisite profilleri sergilememiştir. Ancak; genellikle yüksek fenolik içeriğe sahip güçlü antioksidan aktiviteler sergilemektedirler.

Anahtar Kelimeler

Antioksidan, meme kanseri, rumex, sitotoksisite, total fenol

Proje Numarası

THD-2022-20462

Kaynakça

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