KOJİK ASİT İLE LAVANDULA ANGUSTIFOLIA L. UÇUCU YAĞININ ANTİMİKROBİYAL KOMBİNASYONU

Öz

Amaç: Lamiaceae familyasına ait bir bitki olan Lavandula angustifolia L. (Lavanta), kozmetik, yiyecek ve biyolojik etkilerinden dolayı en çok kullanılan uçucu yağlardan birisidir. Kojik asit beyazlatıcı etkisi için kozmetikte ve antimikrobiyal, antibakteriyel, antiviral gibi farmasötik etkilerinden dolayı kullanılmaktadır. Bu çalışmanın amacı Farmakope kalitesindeki Lavandula angustifolia uçucu yağının kimyasal kompozisyonunu belirlemektir. Buna ek olarak cilt patojenlerine karşı antimikrobiyal aktivitesi ve kojik asit ile uçucu yağın aktivite olarak antibakteriyel sinerjisi incelenmiştir. Gereç ve Yöntem: Bu çalışmada, uçucu yağın kimyasal bileşimi araştırılmıştır. Uçucu yağın içerdiği ana bileşenler GK-AİD ve GK-KS ile linalil asetat (%43,3) ve linalol (%38,6) olarak tespit edilmiştir. Antimikrobiyal aktivite Candida albicans, C. glabrata, Staphylococcus aureus ve Salmonella typhimurium'a karşı değerlendirildi. Sonuç ve Tartışma: Uçucu yağ ve kojik asit C. albicans ve S. aureus'a karşı zayıf antimikrobiyal etki gösterdi. MİK değerleri S. typhimurium'a karşı uçucu yağda 10 mg/ml ve kojik asit 1,25 mg/ml olarak hesaplandı. Sinerjik aktiviteyi değerlendirmek için dama tahtası mikrodilüsyon yöntemi ile uçucu yağ kojik asit ile S. typhimurium'a karşı birleştirildi. Test edilen cilt patojen mikroorganizmalar arasında S. typhimurium, kojik aside karşı daha duyarlıdır. Bu nedenle S. typhimurium'a karşı sinerjik aktivite araştırıldı ve bağımsız etki gözlemlenmiştir.

Anahtar Kelimeler

• Antimikrobiyal aktivite
• kojik asit
• Lavandula angustifolia
• sinerjik aktivite

ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID

Öz

Objective: Lavandula angustifolia L. (Lavender) is one of the most plants essential oils used in the cosmetic, food and biological activities. Kojic acid has been used in cosmetics for its whitening effect and pharmaceutical activity. The aim of this study to determine the composition of the Pharmacopoeia quality L. angustifolia essential oil (EO). Moreover, antimicrobial activities against skin pathogens and synergistic antibacterial activity were also examined of EO and kojic acid. Material and Method: In this work, chemical composition of the EO was defined. Linalyl acetate (43.3%) and linalool (38.6%) were determined as the major components by GC-MS and GC-FID, simultaneously. The antimicrobial activity was evaluated against Candida albicans, C. glabrata, Staphylococcus aureus and Salmonella typhimurium. Result and Discussion: EO and kojic acid showed weak antimicrobial effects. MIC values were determined as the EO 10 mg/ml and kojic acid 1.25 mg/ml against S. typhimurium. To assess the synergistic activity was evaluated by the checkerboard microdilution assay, EO was combined with kojic acid against S. typhimurium. Among the tested skin pathogen microorganisms, S. typhimurium was more sensitive to kojic acid. Therefore, synergic activity was investigated against S. typhimurium and found indifferent effect.

Anahtar Kelimeler

• Antimicrobial activity
• kojic acid
• Lavandula angustifolia
• synergistic activity

Destekleyen Kurum

Laber Kimya and Sns Gıda and Kozmetik

Etik Beyan

The authors declare that the ethics committee approval is not required for this study.

Teşekkür

The authors would like to thank Prof. Dr. Fatih Demirci (Anadolu University, Eskisehir) for supporting this work and the Laber Kimya and Sns Gıda and Kozmetik for financial supports.

Kaynakça

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