KOENZİM Q10 YÜKLÜ MİSEL İÇEREN KONTAKT LENSLERİN HAZIRLANMASI VE İN-VİTRO KARAKTERİZASYONU

Öz

Amaç: Koenzim Q10 (CoQ10), göz sağlığı için potansiyel terapötik faydalar sunmakta, ancak göze uygulandığında düşük çözünürlük ve biyoyararlanım sorunlarıyla karşı karşıya kalmaktadır. Bu çalışma, CoQ10 taşıyan polimerik miselleri Pluronic F127 ve çözücü buharlaştırma tekniği kullanarak kontakt lenslere yükleyerek CoQ10 teslimatını artırmayı amaçlamaktadır Gereç ve Yöntem: Pluronic F127 ile çözücü buharlaştırma yöntemi kullanılarak CoQ10 kapsüllenmiş polimerik miseller üretildi. Ticari kontakt lensler daha sonra bu misellerle yüklendi. Yüklenmiş lenslerin karakterizasyonu, ışık geçirgenliği, şişme davranışı ve göz yüzeyinin kısıtlamalarını taklit eden olmayan emilim koşulları altında ilaç salım profili değerlendirmelerini içermektedir. Sonuç ve Tartışma: Yüklenmemiş lensler %91.78±3.29 ışık geçirgenliği ve %47.51±4.45 şişme oranı sergilerken, misel yüklü lensler yüksek ışık geçirgenliği seviyeleri (%95.31±0.80) göstererek optik berraklığı sağlamıştır. Şişme çalışmaları boyutta hafif bir artışa (%48.1±4.4) işaret etmektedir. Lenslere, etkili bir şekilde 403.6±21.8 µg CoQ10 yüklenmiştir. İn vitro salım profili altı saat boyunca kontrollü salım göstermiş, sürekli ilaç salımı için potansiyel olduğu belirtilmiştir. Bu sonuçlar, etkin oküler ilaç taşıma için misel yüklü kontakt lenslerin uygulanabilirliğini vurgulamakta ve uzun vadeli etkinlik ve güvenliklerinin daha fazla araştırılmasını gerektirmektedir.

Anahtar Kelimeler

• Çözünürlük arttırma
• koenzim Q10
• kontakt lens
• polimerik misel

Proje Numarası

2022/70

PREPARATION AND IN-VITRO CHARACTERIZATION OF CONTACT LENSES CONTAINING COENZYME Q10 LOADED MICELLES

Öz

Objective: Coenzyme Q10 (CoQ10) offers potential therapeutic benefits for ocular health, yet faces challenges of poor solubility and bioavailability when applied to the eye. This study aimed to enhance CoQ10 delivery using contact lenses by incorporating CoQ10-loaded polymeric micelles, using Pluronic F127 and solvent evaporation technique. Material and Method: Polymeric micelles encapsulating CoQ10 were produced via solvent evaporation with Pluronic F127. Commercial contact lenses were subsequently loaded with these micelles. Characterization of the loaded lenses included assessments of light transmittance, swelling behavior, and drug release profile under non-sink conditions, simulating the constraints of the ocular surface. Result and Discussion: The unloaded lenses exhibited a light transmittance of 91.78±3.29% and swelling percentage of 47.51±4.45% while micelle-loaded lenses demonstrated high light transmittance levels (95.31±0.80%), ensuring optical clarity. Swelling studies showed a slight increase in size to 48.1±4.4%. The lenses effectively encapsulated 403.6±21.8 µg of CoQ10. In vitro release profile exhibited controlled release over six hours, indicating potential for sustained drug delivery. These results highlight the feasibility of micelle-loaded contact lenses for efficient ocular drug delivery, warranting further exploration into their long-term effectiveness and safety.

Anahtar Kelimeler

• Coenzyme Q10
• contact lens
• polymeric micelle
• solubility enhancement

Destekleyen Kurum

Trakya Üniversitesi Bilimsel Araştırma Proje Birimi

Proje Numarası

2022/70

Teşekkür

Trakya University Scientific and Technological Research Council has provided the grant this research under the grant 2022/70. The author is grateful to Dr.Burcu Uner for size measurements.

Kaynakça

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