DETERMINATION OF ANTIOXIDANT ACTIVITY OF HIPPOPHAE RHAMNOIDES L. GROWING IN TURKEY AND INVESTIGATION OF ITS EFFECTS ON THE LIVER TOXICITY IN RATS

Öz

Objective: The goal of the present work was to investigate the antioxidant properties of the extracts of Hippophae rhamnoides L. and to determine their effects on liver toxicity in rats. Material and Method: Metal chelation, reducing power and DPPH radical scavenging methods were used in the antioxidant activity analysis of extracts. The total phenolic content was determined using the folin-ciocalteu reagent. Plant extracts were administered orally to the rats at doses of 500 µg/kg for 2 days. Each animal group was composed of six female Albino Wistar rats with an average weight of 250 g. Microscopic examination was carried out to observe any pathological changes in the rat livers. Result and Discussion: Water extract showed the highest radical scavenging activity (48.65%), reducing power (0.291 absorbance at 700 nm) and metal chelating (35.40%) at 1 mg/ml concentration. Histopathological studies showed that especially water extract reduced the severity of CCl4-induced intoxication. Hippophae rhamnoides L. extracts were found to have antioxidant activity, and also Hippophae rhamnoides L. water extract was shown to be particularly effective in preventing liver damage.

Anahtar Kelimeler

• Antioxidant activity
• DPPH
• Hippophae rhamnoides L.
• rat liver toxicity
• reducing power

TÜRKİYE’DE YETİŞEN HİPPOPHAE RHAMNOİDES L. BİTKİSİNİN ANTİOKSİDAN AKTİVİTESİNİN BELİRLENMESİ VE SIÇANLARDA KARACİĞER TOKSİSİTESİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI

Öz

Amaç: Bu çalışmanın amacı Hippophae rhamnoides L. meyve ekstraktlarının antioksidan özelliklerini araştırmak ve sıçanlarda oluşturulan karaciğer toksisitesi üzerindeki etkilerini belirlemektir. Gereç ve Yöntem: Ekstraktların antioksidan aktivite analizinde metal iyonu kelatlama, indirgeme kuvveti ve DPPH radikal tutuklama yöntemleri kullanıldı. Toplam fenolik bileşik miktarları folin-ciocalteu ayıracı ile tespit edildi. Bitki ekstraktları 2 gün süre ile 500 µg/kg doz olacak şekilde ratlara oral olarak verildi. Her bir hayvan grubu ortalama ağırlığı 250 g olan altı adet dişi Albino Wistar sıçanlardan oluşturuldu. Sıçanların karaciğerlerinde herhangi bir patolojik değişiklik olup olmadığını gözlemlemek için mikroskobik inceleme yapıldı. Sonuç ve Tartışma: Su ekstraktı, 1 mg/ml derişimde en yüksek radikal giderme aktivitesini (%48.65), indirgeme gücünü (700 nm'de 0.291 absorbans) ve metal iyonu kelatlamayı (%35.40) gösterdi. Histopatolojik çalışmalar özellikle su ekstraktının CCl4 kaynaklı intoksikasyonun şiddetini azalttığını göstermiştir.

Anahtar Kelimeler

• Antioxidan aktivite
• DPPH
• Hippophae rhamnoides L.
• indirgeme kuvveti
• rat karaciğer toksisitesi

Kaynakça

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