BİSFENOL A VE BİSFENOL S’NİN MCF7 HÜCRE HATTINDA NEDEN OLDUĞU DNA HASARININ ARAŞTIRILMASI

Öz

Amaç: Bu çalışmada, Bisfenol A (BPA) ve Bisfenol S (BPS)'nin MCF7 hücre hattında neden olduğu DNA hasarının belirlenmesi amaçlanmıştır. Gereç ve Yöntem: DNA hasarı BPA ve BPS’nin 0.1, 0.5, 1, 5, 10 ve 50 μM konsantrasyonlarda MCF7 hücre hattında COMET yöntemi ile belirlenmiştir. Sonuç ve Tartışma: Çalışılan tüm BPA ve BPS konsantrasyonları (0.1, 0.5, 1, 5, 10 ve 50 μM) MCF7 hücre hattında kontrole kıyasla önemli ölçüde DNA hasarına neden olmuştur (p<0.05). BPS, çalışılan en yüksek 3 konsantrasyonda (5, 10 ve 50 μM) DNA hasarını BPA'dan daha fazla indüklemiştir (p<0.05). Bu çalışma bisfenol türevlerinin de BPA gibi DNA hasarına neden olabileceğini göstermektedir.

Anahtar Kelimeler

• Bisfenol A
• bisfenol S
• COMET testi
• DNA hasarı
• MCF7 hücre hattı

DETERMINATION OF DNA DAMAGE INDUCED BY BISPHENOL A AND BISPHENOL S IN MCF7 CELL LINE

Öz

Objective: This study aimed to determine the DNA damage induced by Bisphenol A (BPA) and Bisphenol S (BPS) on MCF7 cell line. Material and Method: DNA damage was determined by COMET assay in MCF7 cell line at 0.1, 0.5, 1, 5, 10 and 50 μM concentrations of BPA and BPS. Result and Discussion: All BPA and BPS concentrations studied (0.1, 0.5, 1, 5, 10 and 50 μM) significantly induced DNA damage on MCF7 cell line compared with control (p<0.05). BPS significantly induced DNA damage more than BPA at the 3 highest concentrations studied (5, 10 and 50 μM) (p<0.05). This study shows that bisphenol derivatives can also cause DNA damage like BPA.

Anahtar Kelimeler

• Bisphenol A
• bisphenol S
• COMET assay
• DNA damage
• MCF7 cell line

Kaynakça

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