SİLDENAFİL CD İLE İNDÜKLENEN AKUT TOKSİSİTEDE TNF-α VE IL-6 DÜZEYLERİNİ DÜŞÜRÜR

Öz

Amaç: Bu çalışmada, dişi sıçanlarda kadmiyum (Cd) kaynaklı toksisitede sildenafilin (SIL) inflamasyon ve histopatolojik değişiklikler üzerindeki etkilerinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: İnflamasyon derecesini değerlendirmek için interlökin-6 (IL-6) ve tümör nekroz faktörü-alfa (TNF- α) seviyeleri ve karaciğer, akciğer ve böbreklerdeki histopatolojik değişiklikler değerlendirilmiştir. Sonuç ve Tartışma: SIL, Cd ile indüklenen doku hasarının patogenezinde rol oynayan TNF-α ve IL-6'nın hücresel salınımını önemli ölçüde azaltmıştır. SIL tek başına uygulandığında, kontrol grubuna benzer histopatolojik etkiler göstermiştir. Bununla birlikte, SIL'in Cd ile birlikte uygulanmasının karaciğerde portal ven genişlemesini ve merkezi ven büyümesini önlediği, böbrek dokusunda nekrozu önlediği, ancak akciğeri etkilemediği bulunmuştur. SIL'in dokular üzerinde değişken koruyucu etkileri olmasına rağmen, sonuçlarımız doku hasarı yönetiminde SIL kullanımının in vivo oksidatif stres kaynaklı proinflamatuar sitokin aktivasyonunu modüle etmedeki etkinliğinin araştırılabileceği ve nihayetinde Cd kaynaklı doku hasarını önlemeye yardımcı olabileceği fikrini desteklemektedir. Çalışmamız, SIL'in sıçanlarda Cd kaynaklı akut toksisiteyi azaltabildiğini göstermiştir. SIL, ağır metallerin toksisitesine karşı koruyucu bir ajan olarak kullanılabilir.

Anahtar Kelimeler

• Doku hasarı
• kadmiyum
• karaciğer enzimleri
• oksidatif stres
• sildenafil

SILDENAFIL DECREASED TNF-α AND IL-6 LEVELS IN CD‐INDUCED ACUTE TOXICITY

Öz

Objective: This study aimed to evaluate the effects of sildenafil (SIL) on inflammation and histopathological changes in cadmium (Cd)-induced toxicity in female rats. Material and Method: Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF- α) levels were measured to assess the degree of inflammation. Histopathological changes in the liver, lungs and kidneys were also assessed. Result and Discussion: SIL significantly reduced the cellular release of TNF-α and IL-6, which have been implicated in the pathogenesis of Cd-induced tissue damage. When SIL was administered alone, it showed histopathological effects similar to the control group. However, it was found that co-administration of SIL with Cd prevented portal vein dilation and central vein enlargement in the liver, prevented necrosis in kidney tissue, but did not affect the lung. Although SIL has variable protective effects on tissues, our results are in support of the idea that the use of SIL in tissue damage management can be investigated for its efficacy in modulating oxidative stress-induced proinflammatory cytokine activation in vivo and ultimately help prevent Cd-induced tissue damage. Our study has shown that SIL can reduce Cd-induced acute toxicity in rats. SIL may be use as a protective agent against toxicity of heavy metals.

Anahtar Kelimeler

• Cadmium
• liver enzymes
• oxidative stress
• sildenafil
• tissue damage

Kaynakça

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