PHENOLIC AND ANTIOXIDANT PROFILE: FTIR AND LC-MS ANALYSES OF SERAPIAS ORIENTALIS

Yıl 2024, , 853 - 866, 10.09.2024

Erdi Can Aytar , Yasemin Özdener Kömpe

https://doi.org/10.33483/jfpau.1448197

Öz

Objective: The objective of this study is to characterize the aerial parts of S. orientalis using Fourier Transform Infrared (FTIR) spectroscopy, evaluate the phenolic content and antioxidant activity of seeds, stems, and flowers, and conduct quantitative analysis of phenolic compounds using LC-MS/MS.
Material and Method: Fourier Transform Infrared (FTIR) spectroscopy was employed to characterize the aerial parts of S. orientalis. The analysis focused on identifying various functional groups such as -OH vibrations associated with polysaccharides, C-H vibrations from lipids and lignin compounds, and C=O vibrations related to cellulose derivatives. The total phenolic, flavonoid, flavanol, tannin, and proanthocyanidin contents of S. orientalis seeds, stems, and flowers were evaluated using standard analytical methods. DPPH radical scavenging activity was determined to assess antioxidant potential, with IC50 values calculated for each plant part. Quantitative analysis of phenolic compounds in the plant extract was conducted using LC-MS/MS. The abundance of various phenolic acids including P-coumaric acid, trans-ferulic acid, caffeic acid, and vanillic acid was determined. Additionally, other phenolic compounds such as gallic acid, chlorogenic acid, salicylic acid, (+) taxifolin, rutin hydrate, ellagic acid, quercetin dihydrate, and apigenin were also detected and quantified.
Result and Discussion: The evaluation of phenolic content showed differences among different plant parts, with flowers exhibiting the highest total phenolic and proanthocyanidin content. Seeds demonstrated superior DPPH radical scavenging activity. Quantitative analysis of phenolic compounds using LC-MS/MS highlighted the abundance of various phenolic acids and other phenolic compounds in S. orientalis. These findings underscore the potential of S. orientalis as a valuable source of natural antioxidants. Overall, the results suggest that S. orientalis possesses significant phenolic diversity and antioxidant activity, which could contribute to its potential applications in various industries, including pharmaceuticals and nutraceuticals.

Anahtar Kelimeler

FTIR, LC-MS analysis, plant secondary metabolites, quantitative analysis

FENOLİK VE ANTİOKSİDAN PROFİLİ: SERAPIAS ORIENTALIS'İN FTIR VE LC-MS ANALİZLERİ

Öz

Amaç: Bu çalışmanın amacı, S. orientalis'in topraküstü kısımlarını Fourier Dönüşümü Kızılötesi (FTIR) spektroskopisi kullanarak karakterize etmek, tohumların, sapların ve çiçeklerin fenolik içeriğini ve antioksidan aktivitesini değerlendirmek ve LC-MS/MS kullanarak fenolik bileşiklerin nicel analizini yapmaktır.
Gereç ve Yöntem: S. orientalis'in topraküstü kısımlarını karakterize etmek için Fourier Dönüşümü Kızılötesi (FTIR) spektroskopisi kullanıldı. Analiz, polisakkaritlerle ilişkilendirilen -OH titreşimleri, lipitlerden ve lignin bileşiklerinden kaynaklı C-H titreşimleri ve selüloz türevleriyle ilişkilendirilen C=O titreşimleri gibi çeşitli fonksiyonel grupları tanımlamaya odaklandı.S. orientalis tohumlarının, saplarının ve çiçeklerinin toplam fenolik, flavonoid, flavanol, tanin ve proantosiyanidin içeriği standart analitik yöntemler kullanılarak değerlendirildi. Antioksidan potansiyeli değerlendirmek için DPPH radikal temizleme aktivitesi belirlendi ve her bitki kısmı için IC50 değerleri hesaplandı. Bitki ekstraktındaki fenolik bileşiklerin nicel analizi LC-MS/MS kullanılarak gerçekleştirildi. P-kumarik asit, trans-ferulik asit, kafeik asit ve vanilinik asit gibi çeşitli fenolik asitlerin bol miktarda bulunduğu belirlendi. Ayrıca, galik asit, klorojenik asit, salisilik asit, (+) taksifolin, rutin hidrat, ellajik asit, kuersetin dihidrat ve apigenin gibi diğer fenolik bileşikler de tespit edildi ve nicel olarak belirlendi.
Sonuç ve Tartışma: Fenolik içeriğin değerlendirilmesi, farklı bitki parçaları arasında farklılıklar gösterdi, çiçekler en yüksek toplam fenolik ve proantosiyanidin içeriğini sergiledi. Tohumlar üstün DPPH radikal temizleme aktivitesi gösterdi.LC-MS/MS kullanarak fenolik bileşiklerin nicel analizi, S. orientalis'te çeşitli fenolik asitlerin ve diğer fenolik bileşiklerin bol miktarda bulunduğunu vurgulanmaktadır. Genel olarak, sonuçlar S. orientalis'in önemli bir fenolik çeşitliliğe ve antioksidan aktiviteye sahip olduğunu göstermektedir, bu da onun farmasötik ve nutrasötikler dahil çeşitli endüstrilerde potansiyel uygulamalarına katkıda bulunabileceğini düşündürmektedir.

Anahtar Kelimeler

Bitki ikincil metabolitleri, FTIR, LC-MS analizi, nicel analiz

Kaynakça

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