ATRIPLEX HALIMUS YAPRAKLARINDAN ELDE EDİLEN BİYOAKTİF EKSTRAKTLARIN TOPLAM FENOLİK VE FLAVONOİD ÖLÇÜMÜ VE ANTİOKSİDAN AKTİVİTESİ

Öz

Amaç: Bu çalışmada, Atriplex halimus bitkisinin tamamının çeşitli solvent ekstrelerini, in vitro güçlü antioksidan aktivite sergileme yetenekleri ve ayrıca toplam fenolik ve flavonoid içerikleri açısından tarayarak, gıda ve farmasötik formülasyonlarda gelecek yeni antioksidanlar için potansiyel kaynakları belirleme amaçlandı. Gereç ve Yöntem: Etil eter, etil asetat ve n-butanol ekstreleri dahil olmak üzere Atripleks halimus ekstrelerindeki polifenollerin ve flavonoidlerin toplam miktarının yanı sıra bunların bu bitkinin antioksidan aktivitesi üzerindeki karşılık gelen etkilerini belirlemek için geleneksel prosedürler kullanılarak gerçekleştirildi. Sonuç ve Tartışma: Mevcut araştırmada, bütanolik ekstredeki toplam fenolik ve flavonoid içeriğinin 68.20 mg gallik asit eşdeğeri (GAE)/g kuru ekstrakt) ve 439 mg kersetin eşdeğeri (QE)/g kuru ekstre olduğu bulunmuştur. Hidro-alkolik ekstresi, artan polariteye sahip solventler (etil eter, etil asetat ve n-butanol) ile sıvı/sıvı partitisyona tabi tutulmasıyla, serbest radikal DPPH'nin atıkları ve HPTLC'yi ve bunların indirgeme kinetiklerini ortadan kaldırmasıyla ekstre edildi. Butanol ve etil asetat ekstreleri sırasıyla 2.1959 ve 2.4234 mg/ml IC50 değerleriyle DPPH radikali süpürücü aktivitesine sahip olduğu bulundu.

Anahtar Kelimeler

• Antioksidan aktivite
• Atriplex halimus
• biyoaktif ekstre
• DPPH
• fitokimyasal
• quercetin

TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS

Öz

Objective: This study sought to identify potential sources for upcoming novel antioxidants in food and pharmaceutical formulations by screening various solvent extracts from the leaves of Atriplex halimus Lin. for their ability to exhibit strong antioxidant activity in vitro, as well as their total phenolic and flavonoid contents. Material and Method: To determine the total amount of polyphenols and flavonoids in Atriplex halimus extracts, including ethyl ether, ethyl acetate, and n-butanol extracts, as well as their corresponding impact on this plant's antioxidant activity, were carried out using the conventional procedures. Result and Discussion: In the current investigation, total phenolic and flavonoid contents in butanolic extract were found to be 68.20 mg gallic acid equivalent (GAE)/g dry extract) and 439 mg quercetin equivalent (QE)/g dry extract. The hydro-alcoholic extract was extracted by liquid/liquid partition with solvents of increasing polarity: ethyl ether, ethyl acetate and n-butanol) by the free radical DPPH removing garbage and HPTLC as well as their reduction kinetics. It was found that the extract of butanol and ethyl acetate had powerful uplifting power garbage DPPH with IC50 values of 2.1959 and 2.4234 mg/ml, respectively.

Anahtar Kelimeler

• Antioxidant activity
• Atriplex halimus
• bioactive extract
• DPPH
• phytochemical
• quercetin

Kaynakça

1. 1. Slimani, A., Moussaoui, A., Laazouni, A., Hamadi, N. (2016). Phytochemical screening, contribution to the study of the antifungal effect of flavonoids from different parts of Ziziphus lotus of South-West Algeria. Asian Journal of Pharmaceutical and Clinical Research, 10(2), 20.
2. 2. Lim, Y., Lim, T., Tee, J. (2007). Antioxidant properties of several tropical fruits: A comparative study. Food Chemistry, 103, 1003-1008. [CrossRef]
3. 3. Ismail, A., Marjan, Z., Foong, C. (2004). Total antioxidant activity and phenolic content in selected vegetables. Food Chemistry, 87, 581-586. [CrossRef]
4. 4. Benhammou, N., Bekkara, F.A., Panovska, T.K. (2009). Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus. Comptes Rendus Chimie, 12, 1259-1266. [CrossRef]
5. 5. Abd El-Rahman, H.H., Mohamed, M.I., Gehad, A.E.A., Awadallah, I.M. (2006). Ameliorating the anti-nutritional factors effect in Atriplex halimus on sheep and goats by ensiling or polyethylene glycol (PEG) supplementation. International Journal of Agriculture and Biology, 8, 766.
6. 6. Ziane, L., Lazouni, H.A., Moussaoui, A., Namidi, N., Djellouli, M., Belabbes, A. (2015). Flavonoid from methanolic extract of Limoniastrum feei (girard) batt (Plumbaginaceae). Asian Journal of Pharmaceutical and Clinical Research, 8, 218-219.
7. 7. King, M.B., Bott, T.R. (1993). Extraction of Natural Products Using Near- Critical Solvents. Chapman & Hall, Glasgow, p.325.
8. 8. Kumar, G.P., Kumar, S.V., Kumar, A.S. (2017). Quantification of total phenolic and flavonoid contents, and evaluation of free radical scavenging potential of Vernonia cinerea. Asian Pacific Journal of Health Sciences. 4(3), 279-287. [CrossRef]

9. 9. Rajappa, K.C., Sasidhar, B.S., Naika, M., Keri, R.S. (2018). Phytochemical composition of Caesalpinia crista extract as potential source for inhibiting cholinesterase and β-amyloid aggregation: Significance to Alzheimer's disease. Asian Pacific Journal of Tropical Biomedicine. 8(10), 500-512. [CrossRef]
10. 10. Rydberg, J. (2004). Solvent Extraction Principles and Practice. CRC Press, Boca Raton, p.480.
11. 11. Rosset, R., Jardy A., Caude, M. (1991). Chromatographie en phase liquide et supercitique, Masson, Paris, p.919.
12. 12. Hostettman, K., Marston, A., Hostettman, M. (1998). Preparative chromatography techniques: Applications in natural product isolation, Springer: Berlin Heidelberg. p.240.
13. 13. Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200. [CrossRef]
14. 14. Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for the antioxidant activity. Songklanakarin Journal of Sciences and Technology, 26, 211-219.
15. 15. Raquibul Hasan, S.M., Mokarram Hossain, M., Raushanara, A., Mariam, J., Ehsanul Hoque, M., Shafiqur, R. (2009). DPPH free radical scavenging activity of some Bangladeshi medicinal plants. Journal of Medicinal Plants Research, 3(11), 875-879.
16. 16. Mahmoudi, S., Khali, M., Benkhaled, A., Benamirouche, K., Baiti, I. (2016). Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaves extracts from ten Algerian Ficus carica L. varieties. Asian Pacific Journal of Tropical Biomedicine, 6, 239-245. [CrossRef]
17. 17. Ziane, L., Djellouli, M., Berghioua, A. (2021). Short Communication: Chemical composition, antioxidant andantimicrobial activity of Fagonia longispina (Zygophyllaceae) of Algeria. Biodiversitas Journal of Biological Diversity, 22, 3448-3453. [CrossRef]