TÜRKİYE’DE YETİŞEN SCORZONERA TÜRLERİNİN FİTOKİMYASAL ANALİZİ İÇİN VALİDE EDİLMİŞ YPSK YÖNTEMİ

Öz

Amaç: Bu çalışmada, Türkiye, Avrupa, Moğolistan ve Çin gibi yetiştiği ülkelerde sebze ve tıbbi bitki olarak kullanılan Scorzonera L. (Asteraceae) türleri fenolik bileşikleri açısından değerlendirilmiştir. Gereç ve Yöntem: Türkiye’nin farklı bölgelerinden toplanan Scorzonera cinsine ait yirmi-beş örnek, klorojenik asit, hiperozit, izoorientin, orientin, 7-metil izoorientin, izokersetin, luteolin-7-O-β-glikozit, rutin, swertisin, viteksin gibi bazı standart bileşikler kullanılarak yeni geliştirilen ve valide edilen bir Yüksek Performanslı Sıvı Kromatografisi (YPSK) yöntemi ile incelendi. Her standart bileşik için tespit limiti ve tayin limiti hesaplandı. Sonuç ve Tartışma: Toprak üstü kısımların köklere kıyasla fenolik bileşikler açısından çok daha zengin olduğu ortaya konmuştur. Test edilen tüm türlerin toprak üstü kısımları ve köklerinde klorojenik asit tespit edilirken en yüksek miktarda ise Scorzonera kotschyi’nin toprak üstü kısımlarında (1787.26±32.88 µg/g) bulunmuştur. Test edilen türlerin çoğunda değişen miktarlarda hiperozit, izoorientin, izokersetin ve orientin saptanmıştır. Sırasıyla, Scorzonera aucheriana (572.93±0.04 µg/g), Scorzonera laciniata ssp. laciniata (524.07±5.06 µg/g), Scorzonera tomentosa (892.00±4.58 µg/g) ve Scorzonera cana var. jacquiniana (309.23±1.69 µg/g)’nın toprak üstü kısımları bu bileşikleri yüksek miktarlarda içerirken, viteksin, rutin and luteolin-7-O-β-glikozit test edilen türlerde daha düşük oranda tespit edilmiştir.

Anahtar Kelimeler

• Asteraceae
• fitokimyasal analiz
• flavonoit
• Scorzonera
• validasyon

VALIDATED HPLC METHOD TO ANALYZE PHYTOCHEMICAL STRUCTURE OF SCORZONERA SPECIES GROWN IN TÜRKİYE

Öz

Objective: The current study evaluated Scorzonera L. (Asteraceae) species, which are used as vegetables and medicinal plants in different countries where they grow naturally, such as Türkiye, Europe, Mongolia, and China, for their phenolic composition. Material and Method: The twenty-five members of the Scorzonera genus, collected from different parts of Turkey, were investigated using a newly developed and validated High-Performance Liquid Chromatography (HPLC) method using some standard compounds, including chlorogenic acid, hyperoside, isoorientin, orientin, 7-O-methyl-isoorientin, isoquercetin, luteolin-7-O-β-glycoside, rutin, swertisin, and vitexin. The limit of detection and quantification levels were determined for each standard compound. Result and Discussion: This study has revealed that the aerial parts are rich in phenolic compounds, with significantly higher amounts than the roots. Chlorogenic acid was detected in aerial parts and roots of all tested species and Scorzonera kotschyi aerial parts contained the highest amount (1787.26±32.88 µg/g). Most of the tested species contained varying amounts of hyperoside, isoorientin, isoquercetin, and orientin. Scorzonera aucheriana (572.93±0.04 µg/g), Scorzonera laciniata ssp. laciniata (524.07±5.06 µg/g), Scorzonera tomentosa (892.00±4.58 µg/g) and Scorzonera cana var. jacquiniana (309.23±1.69 µg/g) aerial parts contain these compounds respectively in higher amount. In contrast, vitexin, rutin and luteolin-7-O-β-glycoside were detected in a relatively small number of the tested species.

Anahtar Kelimeler

• Asteraceae
• flavonoid
• phytochemical analysis
• Scorzonera
• validation

Kaynakça

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