RAPHANUS SATIVUS L. ‘RED MEAT’ KÖKLERİNİN SUBKRİTİK SU VE MİKRODALGA EKSTRELERİNİN KOLİNESTERAZ VE TİROZİNAZ İNHİBİTÖR AKTİVİTESİ

Öz

Amaç: Raphanus sativus L. Red Meat köklerinin mikrodalga destekli ve subkritik su ekstrelerinin kolinesteraz ve tirozinaz inhibitör aktivite potansiyeli araştırılmıştır. Gereç ve Yöntem: Geleneksel çözücü ve gelişmiş ekstraksiyon sistemlerinden elde edilen ekstrelerin toplam fenol, flavonoid ve antosiyanin içeriği spektrofotometrik olarak ölçülmüştür. Asetilkolinesteraz, bütirilkolinesteraz ve tirozinaz inhibitör aktiviteleri, kinetik okumalarla absorbans değişim hızının hesaplanmasıyla araştırılmıştır. Sonuç ve Tartışma: Subkritik su ekstresinin sırasıyla 0.71 mg/ml, 2.13 mg/ml ve 1.21 mg/ml IC50 değerleri ile en yüksek asetilkolinesteraz, bütirilkolinesteraz ve tirozinaz inhibitör aktivitelerini sağladığı bulunmuştur. Toplam fenol (27.57 mg GAE/g) ve flavonoid (4.80 mg QE/g) içeriği analizi ile de tutarlı olarak, kırmızı etli turpun subkritik su ekstresi enzim inhibitör aktivite amacıyla üretilen ürünler için potansiyel bir kaynak olarak değerlendirilebilir.

Anahtar Kelimeler

• Karpuz turpu
• kolinesteraz
• mikrodalga
• Raphanus sativus
• subkritik su
• tirozinaz

Proje Numarası

2018-ÖNAP-ECZF-0009

CHOLINESTERASE AND TYROSINASE INHIBITORY ACTIVITY OF SUBCRITICAL WATER AND MICROWAVE EXTRACTS OF RAPHANUS SATIVUS L. ‘RED MEAT’ RADIX

Öz

Objective: Cholinesterase and tyrosinase inhibitory activity potential of microwave-assisted and subcritical water extracts of Raphanus sativus L. Red Meat roots were investigated. Material and Method: Total phenol, flavonoid and anthocyanin content of extracts from conventional solvent and advanced extraction systems were spectrophotometrically quantified. Acetylcholinesterase, butyrylcholinesterase and tyrosinase inhibitory activities were investigated with the calculation of the rate of absorbance change with kinetic readings. Result and Discussion: The subcritical water extract was found to provide highest acetylcholinesterase, butyrylcholinesterase and tyrosinase inhibitory activities with an IC50 of 0.71 mg/ml, 2.13 mg/ml and 1.21 mg/ml, respectively. Consistent with the anaylsis of total phenol (27.57 mg GAE/g) and flavonoid (4.80 mg QE/g) contents, subcritical water extract of red meat radish can be considered as a potential source for products aiming enzyme inhibitory activity.

Anahtar Kelimeler

• Cholinesterase
• microwave
• Raphanus sativus
• red meat radish
• subcritical water
• tyrosinase

Destekleyen Kurum

This work was a part of a project supported by Izmir Katip Celebi University Scientific Research Projects Funds [2018-ÖNAP-ECZF-0009]

Proje Numarası

2018-ÖNAP-ECZF-0009

Etik Beyan

The author declares that the ethics committee approval is not required for this study.

Teşekkür

Access to the facilities of Novel Fluidic Technologies Laboratory and Pharmaceutical Sciences Research Center (FABAL) at Ege University, and Central Research Laboratories (MERLAB) at Izmir Katip Celebi University are highly appreciated.

Kaynakça

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