DOBUTAMİN TAYİNİ İÇİN MIP ESASLI ELEKTROKİMYASAL SENSÖRÜN GELİŞTİRİLMESİ

Öz

Amaç: En yaygın sentetik katekolaminlerden biri olan dobutamin (DBT), renal, kardiyovasküler, hormonal ve merkezi sinir sistemlerinde önemlidir. Çalışmamızda DBT analizi için ilk MIP tabanlı elektrokimyasal sensörün üretilmesi önerilmiştir. MIP(DBT)/GCE, 4-aminobenzoik asidin (4-ABA) DBT varlığında elektropolimerleştirilmesiyle geliştirildi. Analitik performans ve doğrulama değerlendirmesi hem standart çözelti hem de ticari serum örneklerinde gerçekleştirildi. Gereç ve Yöntem: DBT, Vem ilaç firmasından temin edilmiştir. 5 mM [Fe(CN)6]3-/4- çözeltisinin hazırlanması için, potasyum ferrisiyanür ([K3Fe(CN)6]), potasyum ferrosiyanür (K4[Fe(CN)) 6].3H2O, 0.1 M KCl'de karıştırıldı. DBT varlığında polimerik filmi oluşturmak için 4-ABA kullanıldı. Elektrokimyasal ölçümler, IVIUM kompaktStat.h potansiyostatta (Eindhoven, Hollanda) Pt teli, Ag/AgCl referans elektrotu ve camsı karbon elektrottan (GCE) oluşan üç elektrotlu bir sistem kullanılarak gerçekleştirildi. Sonuç ve Tartışma: MIP(DBT)/GCE sensörü, DBT'yi belirlemek için standart çözeltide ve insan serumunun ticari numunelerinde test edildi. DBT konsantrasyonları ΔI değerlerine göre çizildiğinde her iki ortamda da 1×10-13 ile 1×10-12 M arasında doğrusal bir yanıt elde edildi. TS sonuçları ise sırasıyla standart çözelti ve ticari insan serumda 0.087×10-13 ve 0.033×10-13 olarak bulundu. %Geri kazanım ve %BSS sırasıyla %99.68-100.32 ve %1.38-1.66 olarak belirlendi.

Anahtar Kelimeler

• Dobutamin
• elektrokimyasal MIP sensör
• insan serumunun ticari örnekleri
• tayin
• validasyon

THE FABRICATION OF MIP BASED ELECTROCHEMICAL SENSOR FOR THE DETERMINATION OF DOBUTAMINE

Öz

Objective: Dobutamine (DBT), one of the most common synthetic catecholamines, is important in the renal, cardiovascular, hormonal, and central nervous systems. In our study, it is proposed to produce the first MIP-based electrochemical sensor for DBT analysis. MIP(DBT)/GCE was developed by electropolymerization of 4-aminobenzoic acid (4-ABA) in the presence of DBT. Analytical performance and validation evaluation were performed on both standard solution and commercial serum samples. Material and Method: DBT was supplied by the Vem pharmaceutical company. For the preparation of 5 mM [Fe(CN)6]3-/4- solution, known amounts of potassium ferricyanide ([K3Fe(CN)6]) and potassium ferrocyanide (K4[Fe(CN)6].3H2O mixed in 0.1 M KCl solution. The 4-ABA was used to create the polymeric film in the presence of DBT. Electrochemical measurements were actualized in IVIUM compactStat.h potentiostat (Eindhoven, The Netherlands) using a three-electrode system consisting of Pt wire, Ag/AgCl reference electrode, and glassy carbon electrode (GCE). Result and Discussion: The MIP(DBT)/GCE sensor was applied to standard solution and commercial serum samples. When DBT concentrations were plotted against ΔI values, a linear response between 1×10-13 and 1×10-12 M was obtained in both environments. LOD results were found to be 0.087×10-13 M and 0.033×10-13 M in standard solution and commercial human serum, respectively. Recovery% and RSD% were found to be 99.68-100.32% and 1.38-1.66%, respectively.

Anahtar Kelimeler

• Commercial samples of human serum
• determination
• dobutamine
• electrochemical MIP sensor
• validation

Kaynakça

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