IN VITRO EVALUATION OF THE ANTIBACTERIAL EFFECT OF CHITOSAN COATED TEICOPLANIN-LOADED LIPID NANOPARTICLES AGAINST STAPHYLOCOCCUS AUREUS

Yıl 2024, , 1050 - 1057, 10.09.2024

Berrin Küçüktürkmen , Merve Eylül Kıymacı

https://doi.org/10.33483/jfpau.1512913

Öz

Objective: The aim of this research is to formulate teicoplanin-loaded solid lipid nanoparticles (SLN) coated with chitosan to sustain teicoplanin release for effective antibacterial therapy.
Material and Method: “Double emulsion-solvent evaporation technique was used for the production of SLNs. The nanoparticles were characterised in terms of morphology, size, encapsulation efficacy, in-vitro drug release and antibacterial activity studies after optimization of process and formulation parameters.
Result and Discussion: Transmission electron microscopy images confirmed the formation of spherical SLNs. With chitosan coating, the size increased (from 80 nm to 106 nm) and the negative value of zeta potential (- 11.29) changed to positive (+34.41). The in-vitro release data showed prolonged release of teicoplanin from optimized SLN and chitosan-coated SLN (c-SLN) formulations over 1 week. The antibacterial activity study (S. aureus and Methicillin-resistant S. aureus) showed the activity of the teicoplanin loaded SLN formulations. In conclusion, this study demonstrated the potential of c-SLN for effective delivery of teicoplanin.

Anahtar Kelimeler

Chitosan, coating, lipid nanoparticle, precirol ATO 5, teicoplanin

KİTOSAN KAPLI TEİKOPLANİN YÜKLÜ LİPİD NANOPARTİKÜLLERİN STAPHYLOCOCCUS AUREUS'A KARŞI ANTİBAKTERİYEL ETKİSİNİN İN VİTRO DEĞERLENDİRİLMESİ

Öz

Amaç: Bu araştırmanın amacı, etkili antibakteriyel tedavi için teikoplanin salımını uzatmak üzere kitosanla kaplanmış teikoplanin yüklü katı lipit nanopartiküllerinin (SLN) formüle edilmesidir.
Gereç ve Yöntem: SLN'ler çift emülsifikasyon-solvent buharlaştırma tekniği ile hazırlanmıştır. Proses ve formülasyon parametrelerinin optimizasyonu sonrasında nanopartiküller morfoloji, boyut, enkapsülasyon etkinliği, in vitro etken madde salımı ve antibakteriyel aktivite çalışmaları açısından karakterize edilmiştir.
Sonuç ve Tartışma: Geçirimli elektron mikroskobu görüntüleri küresel SLN'lerin oluşumunu doğrulamıştır. Kitosan kaplama ile boyut artmış (80 nm'den 106 nm'ye) ve zeta potansiyelinin negatif değeri (-11.29) pozitife (+34.41) dönüşmüştür. İn vitro salım verileri, optimize edilmiş SLN ve kitosan kaplı SLN (c-SLN) formülasyonlarından teikoplaninin 1 hafta boyunca uzun süreli salınımını göstermiştir. Antibakteriyel aktivite çalışması (S. aureus ve Metisiline dirençli S. aureus), teikoplanin yüklü SLN formülasyonlarının aktivitesini göstermiştir. Sonuç olarak, bu çalışma c-SLN'nin teikoplaninin etkin verilişi için potansiyelini ortaya koymuştur.

Anahtar Kelimeler

Kaplama, kitosan, lipit nanopartikül, precirol ATO 5, teikoplanin

Kaynakça

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