ANTİ-KANSER İLACI PALBOCICLIB'İN BOR KATKILI ELMAS ELEKTROTTA DİFERANSİYEL PULS VOLTAMETRİ YÖNTEMİ KULLANILARAK İNSAN BİYOLOJİK SIVILARINDAN TAYİNİ

Öz

Amaç: Bu çalışmada Palbociclib'i doğru bir şekilde ölçmek için bor katkılı elmas elektrotta (BDDE) diferansiyel puls voltametrisi (DPV) kullanan çok verimli ve hassas bir elektrokimyasal teknik geliştirilmiştir. Gereç ve Yöntem: Tüm deneylerde bor katkılı elmas elektrot, platin tel karşı elektrot ve Ag/AgCl referans elektrot ile birlikte 10 ml kapasiteli tipik üç elektrotlu hücre kullanılmıştır. Elektrokimyasal ölçümler sırasında BDDE'de DPV ve döngüsel voltametri (CV) yöntemleri kullanılmıştır. Sonuç ve Tartışma: Elektrokimyasal karakterizasyon incelemelerinden elde edilen deneysel bulgulara dayanarak, Palbociclib'in BDDE'deki oksidasyon davranışının geri dönüşümsüz olduğu ve difüzyonla düzenlendiği belirlenmiştir. Anodik pik akımı, referans madde çözeltisi, insan serumu ve idrar numuneleri için DPV yöntemiyle pH 2.0 fosfat tampon çözeltisinde (PBS) sırasıyla 0.01-1 µM, 0.02-0.8µM ve 0.02-0.8 µM konsantrasyon aralığında doğrusal bir ilişki görülmüştür Tespit limitleri standart ilaç çözeltisi, insan serumu ve idrar numuneleri için sırasıyla 2.28 nM, 2.93 nM ve 1.31 nM olarak bulunmuştur. Geliştirilen yöntemin validasyonu için tekrarlanabilirliği, tekrar üretilebilirliği, seçiciliği, kesinliği ve doğruluğu araştırılmış ve hesaplanmıştır. Bu yöntem insan serum ve idrar örneklerinde Palbociclib analizi için başarıyla uygulanmıştır.

Anahtar Kelimeler

• Bor katkılı elmas elektrot
• diferansiyel puls voltammetri
• idrar örneği
• insan serum örneği
• palbociclib

DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE

Öz

Objective: A very efficient and sensitive electrochemical technique utilizing differential pulse voltammetry (DPV) at a boron-doped diamond electrode (BDDE) was devised to measure Palbociclib in this study. Material and Method: All experiments employed typical three-electrode cell of 10 ml capacity in conjunction with boron-doped diamond electrode, a platinum wire counter electrode, and an Ag/AgCl reference electrode. During electrochemical measurements, DPV and cyclic voltammetry (CV) methods was utilized at BDDE. Result and Discussion: Based on experimental findings from electrochemical characterization investigations, it was determined that oxidation behavior of Palbociclib in BDDE is irreversible and regulated by diffusion. Anodic peak current exhibited a linear relationship within concentration range of 0.01–1 µM, 0.02–0.8 µM, and 0.02–0.8 µM in pH 2.0 phosphate buffer solution (PBS) for reference substance solution, human serum, and urine samples, respectively. Limits of detection were found as 2.28 nM, 2.93 nM, and 1.31 nM for standard drug solution, human serum and urine samples, respectively. In order to validate the developed method, its repeatability, reproducibility, selectivity, precision and accuracy in all environments were investigated and calculated. This method was successfully applied for the analysis of Palbociclib in human serum and urine samples .

Anahtar Kelimeler

• Boron-doped diamond electrode
• differential pulse voltammetry
• human serum sample
• palbociclib
• urine sample

Kaynakça

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