APIACEAE BİTKİLERİNDEN ELDE EDİLEN DOĞAL KUMARİNLERİN ANTİBAKTERİYEL AKTİVİTELERİNİN DEĞERLENDİRİLMESİ

Öz

Amaç: Bu çalışmanın amacı, on dört doğal kumarin türevinin Staphylococcus epidermidis (ATCC 12228), Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 8739) ve Salmonella enterica serovar Typhimurium (ATCC 14028) bakteri suşlarına karşı antibakteriyel aktivitesini değerlendirmektir. Gereç ve Yöntem: On dört doğal kumarinin antibakteriyel aktivitesi: 4’-senecioyloxyostol (1), deltoin (2), smyrnioridin (3), karatavicinol (4), badrakemin (5), colladonin (6), badrakemone (7), colladonin acetate (8), anatolicin (9), 14’-hydroxycolladonin (10), 14’-hydroxybadrakemin (11), 14’-acetoxycolladonin (12), 14’-acetoxybadrakemone (13) ve 14’-acetoxybadrakemin (14) disk difüzyon yöntemi kullanılarak belirlenmiştir. Sonuç ve Tartışma: Test edilen mikroorganizmalar arasında, Staphylococcus epidermidis kumarin türevlerine karşı en yüksek duyarlılığı gösterirken, Escherichia coli en az duyarlılık gösteren suş olarak belirlenmiştir. Colladonin ve colladonin asetat, sırasıyla E. coli ve S. enterica serovar Typhimurium bakterilerine karşı antibakteriyel aktivite göstermezken, test edilen diğer tüm bakteri suşlarına karşı antibakteriyel aktivite göstermiştir. Özellikle, 4’-senecioyloxyostol 64 µg/disk konsantrasyonunda en güçlü antibakteriyel aktiviteyi 6.6 -7.5 mm zon çapları ile göstermiştir.

Anahtar Kelimeler

• Antibakteriyel
• Apiaceae
• biyolojik aktivite
• disk difüzyon yöntemi
• kumarin

EVALUATION OF THE ANTIBACTERIAL ACTIVITIES OF NATURAL COUMARINS FROM APIACEAE PLANTS

Öz

Objective: This study aimed to determine the antibacterial activity of fourteen natural coumarin derivatives against various bacterial strains including Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 6538), Staphylococcus epidermidis (ATCC 12228), Escherichia coli (ATCC 8739), and Salmonella enterica serovar Typhimurium (ATCC 14028). Material and Method: The antibacterial activity of the following fourteen natural coumarins: 4’-senecioyloxyostol (1), deltoin (2), smyrnioridin (3), karatavicinol (4), badrakemin (5), colladonin (6), badrakemone (7), colladonin acetate (8), anatolicin (9), 14’-hydroxycolladonin (10), 14’-hydroxybadrakemin (11), 14’-acetoxycolladonin (12), 14’-acetoxybadrakemone (13), and 14’-acetoxybadrakemin (14) was investigated by the disc diffusion method. Result and Discussion: Among the microorganisms tested, Staphylococcus epidermidis showed the highest sensitivity to coumarin derivatives, while Escherichia coli was the least sensitive. Colladonin and colladonin acetate showed activity against all bacterial strains tested, except E. coli and S. enterica serovar Typhimurium, respectively. In particular, 4’-senecioyloxyostol exhibited the strongest antibacterial activity at a concentration of 64 µg/disc, with zones of inhibition ranging from 6.6 to 7.5 mm.

Anahtar Kelimeler

• Antibacterial
• Apiaceae
• biological activity
• coumarin
• disc diffusion method

Kaynakça

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