CİSTUS CRETİCUS L.: ANTİOBEZİTE, ANTİMİKROBİYAL VE ANTİBİYOFİLM ÖZELLİKLERİ

Öz

Amaç: Cistus creticus L. Türkiye'nin kıyı bölgelerinde yaygın olarak bulunur. Bu çalışmada, sulu ve etanollü ekstrelerin sitotoksik, antiobezite, antimikrobiyal ve antibiyofilm özelliklerinin yanı sıra toplam fenolik ve flavonoit içeriği in vitro olarak araştırılmıştır. Gereç ve Yöntem: Cistus creticus'un çiçekli toprak üstü kısımlarından hareketle etanol ve su kullanılarak iki farklı ekstre hazırlanmıştır. Toplam fenolik içerik ve toplam flavonoitler sırasıyla Folin-Ciocalteu ve alüminyum klorür kolorimetrik yöntemleriyle belirlenmiştir. Cistus creticus'un toprak üstü kısımlarının etanollü ve sulu ekstrelerinin 3T3-L1 hücre canlılığı üzerindeki etkisi metil tiyazol tetrazolyum (MTT) ile belirlenmiş ve bitki ekstrelerinin 3T3-L1 adipositlerinde farklılaşma ve lipit birikimi üzerindeki etkileri Yağ-Kırmızı O Boyama ile değerlendirilmiştir. Ayrıca, MIC değerleri ve antibiyofilm aktiviteleri de incelenmiştir. Sonuç ve Tartışma: EtOH ve sulu ekstrelerin toplam fenol içeriği sırasıyla 134.2849 mg GAE/g ve 96,1803 mg GAE/g olarak belirlenmiştir. Su ve EtOH ekstrelerindeki toplam flavonoitlerin sırasıyla 33.1942 mgKE/g ve 22.8338 mgKE/g olduğu bulunmuştur. En düşük MİK değerleri Bacillus subtilis DSM 1971, Bacillus licheniformis DSM 13 ve Bacillus amyloliquefaciens DSM 7 suşları için belirlenirken, en yüksek MİK konsantrasyonu Escherichia coli ve Enteroccus gallinarum suşları için bulunmuştur. Pseudomonas aeruginosa ATCC 27853 ve Escherichia coli ATCC 25922'nin MIC/16 konsantrasyonunun da biyofilm oluşumunu engellemede etkili olduğu kanıtlanmıştır. EtOH ekstresi ile muamele edilen 3T3-L1 adipositlerinde lipid birikimi üzerinde belirgin ancak güçlü olmayan etkiler gözlemledik.

Anahtar Kelimeler

• Antibiofilm özellik
• antimikrobiyal aktivite
• antiobezite aktivite
• Cistus creticus
• total fenol içeriği
• toplam flavonoit içeriği

CISTUS CRETICUS L.: ANTIOBESITY, ANTIMICROBIAL AND ANTIBIOFILM PROPERTIES

Öz

Objective: Cistus creticus L. is widespread in the coastal regions of Türkiye. In this study, we investigated the cytotoxic, antiobesity, antimicrobial and antibiofilm properties as well as the total phenolic and flavonoid content of both aqueous and ethanolic extracts in vitro. Material and Method: Two different extracts were prepared from the flowering aerial parts of Cistus creticus using ethanol and water. The total phenolic content and total flavonoids were determined by the Folin-Ciocalteu method and the aluminum chloride colorimetry, respectively. The effect of extracts on the cell viability of 3T3-L1 was determined by methyl thiazole tetrazolium (MTT), and the evaluation of differentiation and the effects of the plant extracts on lipid accumulation in 3T3-L1 adipocytes was performed by Oil-Red O staining. In addition, MIC values and antibiofilm activities were also investigated. Result and Discussion: The total phenol content of the EtOH and water extract was determined to be 134.2849 mg GAE/g and 96.1803 mg GAE/g, respectively. The total flavonoids in the water and EtOH extracts were found to be 33.1942 mgQE/g and 22.8338 mgQE/g, respectively. The lowest MIC values were determined for the strains Bacillus subtilis DSM 1971, Bacillus licheniformis DSM 13 and Bacillus amyloliquefaciens DSM 7, while the highest MIC concentration was found for the strains Escherichia coli and Eenterococcus gallinarum. The MIC/16 concentration of Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 also proved to be effective in inhibiting biofilm formation. We observed that noticeable but not strong effects on lipid accumulation were observed in 3T3-L1 adipocytes treated with EtOH extract.

Anahtar Kelimeler

• Antibiofilm property
• antimicrobial activity
• antiobesity activity
• Cistus creticus
• total flavonoid content
• total phenol content

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