ORİGANUM ONİTES L. ETANOLİK EKSTRAKTI OKSİDAN, İNFLAMATUAR VE APOPTOTİK BELİRTEÇLERİ DÜZENLEYEREK SIÇANLARDA ASETİK ASİT İLE İNDÜKLENEN ÜLSERATİF KOLİTİ HAFİFLETİR

Öz

Amaç: Bu çalışmada Origanum onites etanolik özütünün (O. onites EE) antioksidan ve anti-inflamatuar etkileri in vitro olarak ve inflamasyon, apoptoz, oksidatif stres ve doku hasarıyla ilişkili parametreler üzerindeki etkileri asetik asit (AA) ile indüklenen sıçan ülseratif kolit (UC) modelinde değerlendirilmiştir. Gereç ve Yöntem: O. onites'in kurutulmuş kısımları maserasyon yöntemi kullanılarak etanol ile ekstre edildi. Daha sonra, ekstrenin anti-DPPH ve anti-ABTS serbest radikal temizleme, anti-5-lipoksijenaz aktiviteleri ve toplam sekonder metabolik miktarları ölçüldü. Sıçan grupları kontrol + salin (C), UC + salin (A), UC + O. onites EE (O), UC + Sülfasalazin (S) olarak belirlendi. Tüm tedaviler 3 ardışık gün boyunca günde bir kez uygulandı. Sonuç ve Tartışma: O. onites EE, toplam fenolik, flavonoid ve triterpen içeriklerine iyi miktarda, sahipti ve ABTS, DPPH radikallerine karşı yüksek bir antioksidan aktivite ve 5-LOX enzimine karşı yüksek bir anti-inflamatuar aktivite gösterdi. AA ile uygulanması ile artan luminol-lusigenin, TNF-α, IL-1, IL-17, TLR-9, kaspaz-3, kaspaz-9, MMP-3, makroskobik ve mikroskobik skorlar, O. onites EE ve sülfasalazin tedavileriyle azaldı. Sonuç olarak, O. onites EE'nin sıçanlarda kolit üzerinde iyi bir terapötik etkisi vardır; bu da esas olarak total flavonoid, fenolik ve triterpen içeriği ve Anti-5-LOX ve serbest radikal temizleyici aktivitesinden kaynaklanmaktadır.

Anahtar Kelimeler

• Anti-5-LOX
• apoptoz
• IL-17
• IL-10
• Origanum onites
• TRL-9
• ülseratif kolit

ORIGANUM ONITES L. ETHANOLIC EXTRACT ALLEVIATES ACETIC ACID-INDUCED ULCERATIVE COLITIS IN RATS BY REGULATING OXIDANT, INFLAMMATORY, AND APOPTOTIC MARKERS

Öz

Objective: This study aims to evaluate the antioxidant and anti-inflammatory effects of Origanum onites ethanolic extract (O. onites EE) in vitro and its effects on inflammation, apoptosis, oxidative stress, and tissue damage-related parameters in the acetic acid (AA)-induced ulcerative colitis (UC) model in rats. Material and Method: Dried parts of O. onites were extracted with ethanol using the maceration method. Then, the extract’s anti-DPPH and anti-ABTS free radical scavenging, anti-5-lipoxygenase (LOX) activities, and total secondary metabolic quantities were evaluated in vitro. The rat groups were determined as control + saline (C), AA + saline (A), AA + O. onites EE (O), AA + Sulfasalazine (S). The treatments were applied once a day for 3 consecutive days. Result and Discussion: O. onites EE showed high amount of total phenolic, total flavonoid, and total triterpene contents, high antioxidant activity against ABTS, DPPH radicals, and anti-inflammatory effects against 5-LOX enzyme. Due to AA-induction, increased luminol-lucigenin, TNF-α, IL-1β, IL-17, TLR-9, caspase-3, caspase-9, MMP-3, macroscopic and microscopic scores attenuated by O. onites EE and sulfasalazine treatments. In conclusion, O. onites EE has a significant therapeutic effect on rats' colitis due to its total flavonoid, phenolic, and triterpene content, and its Anti-5-LOX and free radical scavenging activity.

Anahtar Kelimeler

• Anti-5-LOX
• apoptosis
• IL-17
• IL-10
• Origanum onites
• TLR-9
• ulcerative colitis

Etik Beyan

All procedures for experimental protocols of the present study involving animals were performed following the ethical standards of the institution or practice at which the studies were conducted. Approval was granted by the Ethics Committee of University Marmara (Date: 2023, No:50.2023mar). All experimental practices concerning rats were executed following "The Guide for the Care and Use of Laboratory Animals" (www.nap.edu/ catalog/5140.html) and the principles of ARRIVE guidelines.

Kaynakça

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