KORNEA AMELİYATLARI SONRASINDA KULLANILABİLECEK GÖZ KURULUĞUNU ÖNLEYİCİ İN-SİTU HİDROJEL FORMÜLASYONLARININ TASARIMI VE İN VİTRO KARAKTERİZASYONU

Öz

Amaç: Göz kuruluğunu önlemek için kullanılan göz damlalarının gözde kalış süresinin kısa olmasıyla alternatif dozaj şekillerinin araştırılmasını gerektirmiştir. İn-situ hidrojeller, saklama sırasında sol fazında olup çevresel koşullarda jel fazına geçerek hedef bölgede daha uzun süre kalabilmektedir. Bu çalışmada, doğal ve biyouyumu polimerler kullanılarak göz sıcaklığında sol-jel geçişi yapabilen in-situ hidrojel formülasyonları geliştirilmiş ve karakterizasyonları gerçekleştirilmiştir. Gereç ve Yöntem: HA, CMC, PAA ve sitrik asit, fiziksel karıştırma yöntemi esas alınarak 37°C sıcaklıkta distile su içerisine serpilerek homojen dağılımı sağlanmıştır. Göz fizyolojisine uygunluğu karakterizasyon çalışmaları ile kontrol edilmiştir. pH ölçümü, şişme/erozyon ve sol-jel geçiş sıcaklığı değerlendirilmesi ve viskozite ölçümü karakterizsyon çalışmaları altında gerçekleştirilmiştir. Tüm veriler değerlendirilerek optimum özelliklere sahip en uygun formülasyon/lar seçilerek hızlandırılmış stabilite çalışmaları gerçekleştirilmiştir. Sonuç ve Tartışma: Su tutma kapasitesi yüksek, biyouyumlu, gözde uzun süre kalabilen in-situ hidrojel formülasyonları için hyaluronik asit, poliakrilamid ve karboksimetil selüloz kombinasyonu uygun bulunmuştur. 7 günlük hızlandırılmış in vitro stabilite çalışmasında, formülasyonların pH ve viskozite değerlerinde önemli bir değişiklik gözlenmemiştir.

Anahtar Kelimeler

• Hyaluronik asit
• in-situ hidrojel
• karboksimetil selüloz
• kornea
• poliakrilamid

DESIGN AND CHARACTERIZATION OF IN-SITU HYDROGEL FORMULATIONS TO PREVENT DRY EYE AFTER CORNEAL SURGERIES

Öz

Objective: The short retention time of eye drops used to prevent dryness has necessitated the exploration of alternative dosage forms. İn-situ hydrogels remain in a sol phase during storage but transition to a gel phase under environmental conditions, allowing prolonged retention at the target site. In this study, in-situ hydrogel formulations capable of sol-gel transition at ocular temperature were developed using natural and biocompatible polymers, and their characterizations were performed. Material and Method: HA, CMC, PAA, and citric acid were homogeneously dispersed in distilled water at 37°C by the physical mixing method. The suitability for ocular physiology was verified through characterization studies. pH measurement, swelling/erosion assessment, sol-gel transition temperature evaluation, and viscosity measurement were conducted as part of the characterization studies. Based on the evaluation of all data, the most suitable formulation(s) with optimal properties were selected, and accelerated stability studies were performed. Result and Discussion: The combination of hyaluronic acid, polyacrylamide, and carboxymethyl cellulose was found suitable for in-situ hydrogel formulations with high water retention capacity, biocompatibility, and prolonged ocular residence time. During the 7-day accelerated in vitro stability study, no significant changes were observed in the pH and viscosity values of the formulations.

Anahtar Kelimeler

• Carboxymethyl cellulose
• cornea
• hyaluronic acid
• in-situ hydrogel
• polyacrylamide

Kaynakça

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