DETERMINATION OF THE PROTECTIVE EFFECTS OF RESVERATROL AGAINST ALCOHOL-INDUCED INFLAMMATION AND APOPTOSIS IN HUVEC CELL LINE

Öz

Objective: Alcohol is a toxic substance that leads to tissue and organ damage through an inflammatory cascade involving numerous cytokines, chemokines, and reactive oxygen species. Given that chronic inflammation is recognized as a central factor in various diseases, recent studies have focused on foods rich in polyphenols with anti-inflammatory and immunomodulatory properties. This study aims to investigate the protective and therapeutic effects of resveratrol, a widely studied polyphenol, on alcohol-induced human umbilical vein endothelial cell (HUVEC) lines. Material and Method: In our study, 4 different experimental groups were formed as control group, alcohol exposure group, resveratrol group, alcohol and resveratrol co-administered group. The doses to be applied were determined as the inhibitory concentration 30 (IC30) dose (4.4%) for alcohol and the highest non-toxic dose (20 µM) for resveratrol according to the results obtained from cytotoxicity experiments. The study was conducted on human umbilical vein endothelial cell (HUVEC) lines. The parameters examined, including Interleukin 18 (IL-18), C-reactive protein (CRP), and caspase-3 activity, were measured using ELISA kits. Result and Discussion: Resveratrol supplementation has been shown to provide protection against inflammation by significantly reducing the levels of the inflammatory cytokine interleukin 18 (IL-18) in response to alcohol (%20.31, p<0.05). Additionally, resveratrol significantly reduced C-reactive protein (CRP) levels (%17.41, p<0.05). It was also demonstrated that alcohol significantly increased caspase-3 activity in HUVEC cell lines, while resveratrol administration effectively inhibited this increase (%12.75 p<0.05). It is suggested that resveratrol reduces CRP levels and mediates caspase-3 inhibition by alleviating alcohol-induced oxidative stress. Given that resveratrol has not shown any adverse effects on human health and possesses protective properties against oxidative stress, it is considered that it could be used as a nutraceutical supplement in conjunction with pharmaceutical treatments.

Anahtar Kelimeler

• Alcohol
• caspase-3
• CRP
• IL-18
• resveratrol

HUVEC HÜCRE HATTINDA ALKOL İLE İNDÜKLENEN İNFLAMASYON VE APOPTOZA KARŞI RESVERATROLÜN KORUYUCU ETKİLERİNİN BELİRLENMESİ

Öz

Amaç: Alkol çok sayıda sitokin, kemokin ve reaktif oksijen türlerini içeren inflamatuar bir kaskad yoluyla doku ve organ hasarına yol açan toksik bir maddedir. Kronik inflamasyonun çeşitli hastalıklarda merkezi bir rol oynadığı kabul edildiğinden, son yıllarda yapılan çalışmalar anti-inflamatuar ve immünomodülatör özelliklere sahip polifenoller açısından zengin gıdalara odaklanmıştır. Bu çalışmanın amacı, oldukça yaygın şekilde araştırılan bir polifenol olan resveratrolün, alkol ile indüklenen insan umblikal ven endotel (HUVEC) hücre hatları üzerinde koruyucu ve terapötik etkilerinin incelenmesidir. Gereç ve Yöntem: Çalışmamızda, kontrol grubu, alkol maruziyeti grubu, resveratrol grubu, alkol ve resveratrol birlikte uygulanan grup olmak üzere 4 farklı deney grubu oluşturulmuştur. Uygulanacak dozlar, sitotoksisite deneylerinden elde edilen sonuçlara göre alkol için inhibitör konsantrasyon 30 (IC30) dozu (%4,4) ve resveratrol için toksisite göstermeyen en yüksek doz (20 µM) olarak belirlenmiştir. İnsan umblikal ven endotel (HUVEC) hücre hattı üzerinde çalışılmıştır. Çalışmada incelenmiş olan İnterlökin 18 (IL-18), C reaktif protein (CRP), Kaspaz-3 aktivitesi parametreleri ELISA kitleri kullanılarak ölçülmüştür. Sonuç ve Tartışma: Resveratrol takviyesinin alkole karşı, inflamatuar sitokinlerden interlökin 18 (IL-18)’i önemli ölçüde azaltarak inflamasyona karşı koruma sağladığı gösterilmiştir (%20.31, p<0.05). Ek olarak, resveratrol C-reaktif protein (CRP) seviyelerinde önemli bir azalma sağlamıştır (%17.41, p<0.05). Ayrıca HUVEC hücre hattında alkolün kaspaz-3 aktivitesini anlamlı olarak artırdığı, resveratrol uygulanmasının ise bu artışı anlamlı şekilde inhibe ettiği gösterilmiştir (%12.75 p<0.05). Resveratrolün alkolün neden olduğu oksidatif stresi azaltarak CRP seviyelerini azalttığı ve kaspaz-3 inhibisyonuna aracılık ettiği düşünülmektedir. Resveratrolün insan sağlığı üzerine herhangi bir olumsuz etki göstermediği ve oksidatif strese karşı koruyucu özelliklere sahip olduğu görüldüğünden ilaç tedavilerine ek bir nutrasötik olarak kullanılabileceği düşünülmektedir.

Anahtar Kelimeler

• Alkol
• CRP
• IL18
• kaspaz 3
• resveratrol

Kaynakça

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