ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ENDEMIC HAPLOPHYLLUM MYRTIFOLIUM BOISS.

Öz

Objective: Haplophyllum myrtifolium Boiss. an endemic species for Türkiye, belongs to the Rutaceae family. In this study, antioxidant and antimicrobial effects of n-hexane and methanol extracts of the aerial parts were examined. Material and Method: The total phenolic content of the extracts was examined by a spectrophotometric method using Folin-Ciocalteu reagent (FCR). The DPPH free radical scavenging and ABTS test were applied to determine the antioxidant effect. The antibacterial effects of H. myrtifolium extracts were assayed against Stapylococcus aureus ATCC 29213, Bacillus subtilis NRRL B478 and Enterecoccus faecalis ATCC 51299 by the broth microdilution method. Result and Discussion: The total phenolic content was found to be 44.69±0.021 mg GAE/g for the methanol extract. The methanol extract of H. myrtifolium exhibited higher antioxidant activity (IC50 0.120±0.0008 mg/ml) compared to the n-hexane extract (IC50 0.256±0.015 mg/ml). ABTS test results, the data closest to the TEAC standard was seen in methanol extract (1.783±0.0062 mM). The minimum bactericidal concentrations of the H. myrtifolium methanol extracts varied between 0.125-2 mg/ml and the n-hexane extracts showed antimicrobial activity with the 3 mg/ml concentration.

Anahtar Kelimeler

• Antimicrobial activity
• antioxidant activity
• Haplophyllum myrtifolium
• total phenolic content

ENDEMİK HAPLOPHYLLUM MYRTIFOLIUM BOİSS.'UN ANTİMİKROBİYAL VE ANTİOKSİDAN AKTİVİTESİ

Öz

Amaç: Haplophyllum myrtifolium Boiss. Türkiye'ye özgü bir tür olup, Rutaceae familyasına aittir. Bu çalışmada, toprak üstü kısımlarının metanol ve n-hekzan ekstrelerinin antimikrobiyal ve antioksidan etkileri araştırılmıştır. Gereç ve Yöntem: Toplam fenol içeriği, gallik asit eşdeğeri (GAE) olarak Folin-Ciocalteu reaktifi (FCR) kullanılarak belirlenmiştir. Antioksidan etkiyi belirlemek için DPPH ve ABTS testi uygulanmıştır. H. myrtifolium ekstrelerinin antibakteriyel etkileri Stapylococcus aureus ATCC 29213, Bacillus subtilis NRRL B478 ve Enterecoccus faecalis ATCC 51299'a karşı brot mikrodilüsyon yöntemiyle test edilmiştir. Sonuç ve Tartışma: Metanol özütü için toplam fenolik içerik 44.69±0.021 mg GAE/g olarak bulundu. H. myrtifolium metanol özütü, n-hekzan özütüne (IC50 0.256±0.015 mg/ml) kıyasla daha yüksek antioksidan aktivite (IC50 0.120±0.0008 mg/ml) gösterdi. ABTS test sonuçlarına göre TEAC standardına en yakın veri metanol ekstrelerinde (1.783±0.0062 mM) görüldü. H. myrtifolium metanol ekstrelerinin minimum bakterisidal konsantrasyonları 0.125-2 mg/ml arasında değişirken, n-hekzan ekstreleri 3 mg/ml konsantrasyonda antimikrobiyal aktivite göstermiştir.

Anahtar Kelimeler

• Antimikrobiyal aktivite
• antioksidan aktivite
• Haplophyllum myrtifolium
• toplam fenolik içerik

Kaynakça

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