KOLOREKTAL KANSERDE ARTMIŞ ENDOGLİN DÜZEYİ İLE İNFLAMATUVAR TÜMÖR MİKROÇEVRE İLİŞKİSİ

Öz

Amaç: Kolorektal kanser (CRC) dünyada en yaygın görülen kanserlerden biri olup, kansere bağlı ölümlerin önde gelen nedenleri arasındadır. Tümörlerin sıklıkla kronik enflamasyon ortamında geliştiği ve tümör biyopsi örneklerinde enflamatuvar hücrelerin mevcut olduğu gözlemine dayanarak, enflamasyon ve kanser arasında nedensel bir bağlantı öne sürülmüştür. Epidemiyolojik çalışmalar, kronik enflamasyonun farklı kanser türlerine zemin hazırladığını ortaya çıkarmıştır. Kronik inflamasyonun kanser gelişimini destekleyebildiğini ve tümör kaynaklı enflamasyonun kartopu etkisi yaratarak tümör progresyonunu sürdürdüğünü gösteren çok sayıda kanıt bulunmaktadır. Endoglin, vasküler endotel hücrelerinde tanımlanmış bir transmembran glikoprotein olup, endoglinin çeşitli hastalıklarda anjiyogenez ve inflamasyon gelişimi ile ilişkili olduğu gösterilmiştir. Bu çalışmada, kolorektal kanser hastalarında endoglin ile inflamatuvar mikroçevre ilişkisinin araştırılması amaçlandı. Gereç ve Yöntem: Kolorektal kanser tanısı almış 50 hasta ile 50 sağlıklı gönüllü çalışmaya dahil edildi. Plazma örneklerinde, endoglin ve yaygın olarak bilinen enflamasyon belirteçlerinden olan sPLA2, cPLA2, NfκB, TGF-β1 düzeyleri ELISA (enzyme-linked immunosorbent assay) yöntemi ile ölçüldü. Ayrıca, endoglin ve PLA2 mRNA ekspresyon düzeyleri Real-Time PCR ile belirlendi. Sonuç ve Tartışma: Kolorektal kanser hastalarında plazma endoglin, NfκB, TGFβ1 düzeylerinin, kontrol grubuna göre anlamlı olarak yüksek olduğu saptanırken, cPLA2 ve sPLA2 düzeylerinde gruplar arasında anlamlı bir farklılık bulunmadı. Kolorektal kanser grubuna ait plazma endoglin düzeyleri ile NFκB arasında anlamlı pozitif korelasyon saptandı. Gen ekspresyon analizi bulguları, kolorektal kanser grubunda endoglin ve PLA2 mRNA ekspresyon düzeylerinin, kontrol grubuna göre anlamlı olarak yüksek olduğunu gösterdi. Sonuç olarak, bu çalışma endoglinin kolorektal kanserdeki artışının inflamasyon gelişimi ile ilişki olabileceğini ve inflamatuvar mikroçevre ile ilişkili kötü prognozda rol oynayabileceğini göstermiştir.

Anahtar Kelimeler

• Endoglin
• Kolorektal Kanser
• NFκB
• PLA2
• TGFβ1

ELEVATED ENDOGLIN LEVELS AND THEIR LINK TO THE INFLAMMATORY TUMOR MICROENVIRONMENT IN COLORECTAL CANCER

Öz

Objective: Colorectal cancer (CRC) is one of the most common cancers worldwide and is one of the leading causes of cancer-related deaths. The causal link between inflammation and cancer has been proposed based on the observation that tumors often develop in a chronic inflammatory environment and that inflammatory cells are present in tumor biopsy specimens. Epidemiological studies have revealed that chronic inflammation provides a basis for different types of cancer. There is a great deal of evidence to suggest that chronic inflammation can support cancer development and that tumor-induced inflammation maintains tumor progression by creating a snowball effect. Endoglin is a transmembrane glycoprotein identified in vascular endothelial cells and has been shown to be associated with angiogenesis and inflammation in various diseases. This study aimed to investigate the relationship between endoglin and the inflammatory microenvironment in colorectal cancer patients. Material and Method: 50 patients diagnosed with colorectal cancer and 50 healthy volunteers were included in the study. In plasma samples, endoglin and commonly known inflammation markers such as sPLA2, cPLA2, NfκB, and TGFβ1 levels were measured by ELISA (enzyme-linked immunosorbent assay) method. In addition, endoglin and PLA2 mRNA expression were determined by Real-Time PCR. Result and Discussion: In colorectal cancer patients, plasma endoglin, NfκB, TGFβ1 levels were found to be significantly higher than in the control group, while no significant difference was found between the groups in cPLA2 and sPLA2 levels. A significant positive correlation was found between plasma endoglin levels and NFκB in the colorectal cancer group. Gene expression analyses showed that endoglin and PLA2 mRNA expression levels were significantly higher in the colorectal cancer group than in the control group. In conclusion, this study showed that the increase in endoglin in colorectal cancer may be associated with the development of inflammation and may play a role in poor prognosis associated with the inflammatory microenvironment.

Anahtar Kelimeler

• Colorectal cancer
• Endoglin
• NFκB
• PLA2
• TGFβ1

Kaynakça

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