PARONYCHIA ARGENTEA LAM.’IN ANTİOKSİDAN, ANTİDİYABETİK VE ANTİOBEZİTE ETKİ POTANSİYELLERİNİN DEĞERLENDİRİLMESİ

Öz

Amaç: Paronychia Mill. cinsine ait bitkiler dünyanın birçok bölgesinde çeşitli hastalıklara karşı geleneksel olarak kullanılmaktadır. Bu araştırmada Paronychia argentea Lam.’ın toprak üstü kısımlarından hazırlanan ekstrenin antioksidan, antidiyabetik ve antiobezite etkileri araştırılmış ve total fenol ve flavonoid içerikleri analiz edilmiştir. Gereç ve Yöntem: P. argentea’nın toprak üstü kısımlarından hazırlanan %80 etanol ekstresinin antioksidan aktivitesi araştırılırken DPPH ve ABTS radikal süpürücü aktivite, demir indirgeme güç, metal bağlama kapasite ve total antioksidan kapasite yöntemleri kullanılmıştır. Diğer taraftan ekstrenin antidiyabetik etkisi α-glukozidaz, α-amilaz enzim inhibisyon testleri ile antiobezite etkisi ise pankreatik lipaz enzim inhibisyon testi ile belirlenmiştir. P. argentea ekstresinin toplam flavonoit ve toplam fenol içeriği UV spektrofotometrik yöntemlerle değerlendirilmiştir. Sonuç ve Tartışma: P. argentea toprak üstü ekstresinin (2000 µg/ml) metal bağlama kapasitesi ve DPPH radikal süpürücü aktivitesi sırasıyla %34.19 ± 1.68 ve %65.88 ± 1.43 olduğu bulunmuştur. Ekstrenin konsantrasyonu arttıkça tüm enzim sistemlerinde inhibisyon yüzdesinde artış gözlenmiştir. P. argentea ekstresi α-glukozidaz enzimi üzerindeki en güçlü inhibitör etkisini 2000 µg/ml konsantrasyonda (%60.80 ± 1.94) göstermiştir. Ekstrenin 47.69 ± 1.03 mg GAE/g ekstre toplam fenol içeriğine ve 40.42 ± 1.32 mg QE/g ekstre toplam flavonoid içeriğine sahip olduğu tespit edilmiştir. Bulgular sonucunda ekstrenin ılımlı antioksidan aktiviteye sahip olduğu ve enzim sistemleri üzerinde en güçlü inhibitör etkiyi α-glukozidaz enzimine karşı gösterdiği görülmüştür. P. argentea toprak üstü kısımlarından hazırlanan ekstre üzerinde daha ileri antidiyabetik çalışmalarının yapılmasına ihtiyaç vardır.

Anahtar Kelimeler

• Antioksidan
• enzim inhibisyonu
• Paronychia

ASSESSMENT OF THE ANTIOXIDANT, ANTIDIABETIC AND ANTIOBESITY EFFECT POTENTIALS OF PARONYCHIA ARGENTEA LAM.

Öz

Objective: Plants belonging to the genus Paronychia Mill. are traditionally used in many parts of the world against various diseases. In this study, antiobesity, antioxidant and antidiabetic effects of the extract prepared from the aerial parts of Paronychia argentea Lam. were investigated and total phenol and flavonoid contents were analysed. Material and Method: DPPH and ABTS radical scavenging activity, ferric reducing power, metal chelating capacity and total antioxidant capacity methods were used to investigate the antioxidant activity of 80% ethanol extract prepared from the aerial parts of P. argentea. On the other hand, the antidiabetic effect of the extract was determined by α-glucosidase, α-amylase enzyme inhibition tests and antiobesity effect was determined by pancreatic lipase enzyme inhibition test. Total flavonoid and total phenol contents of the P. argentea extract were evaluated by UV spectrophotometric methods. Result and Discussion: The metal chelating capacity and DPPH radical scavenging activity of P. argentea extract (2000 µg/ml) were 34.19 ± 1.68% and 65.88 ± 1.43%, respectively. As the extract concentration increased, an increase in the inhibition percentage was observed in all enzyme systems. P. argentea extract showed the strongest inhibitory effect on α-glucosidase enzyme at 2000 µg/ml (60.80 ± 1.94%). Total phenol content of the extract was 47.69 ± 1.03 mg GAE/g extract and total flavonoid content was 40.42 ± 1.32 mg QE/g extract. The results showed that the extract had moderate antioxidant activity and the strongest inhibitory effect on enzyme systems was on α-glucosidase enzyme. Further antidiabetic studies should be carried out on the extract prepared from the aerial parts of P. argentea.

Anahtar Kelimeler

• Antioxidant
• enzyme inhibition
• Paronychia

Teşekkür

We would like to thank Ayşegül Culu for her help in collecting the plant.

Kaynakça

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