SIÇAN MİKROZOMLARINDA CYP1A1 AKTİVİTESİNİN CİTRUS L. MEYVE SULARI İLE MODÜLASYONU

Öz

Amaç: Citrus L. meyve suları, küresel meyve suyu pazarının yaklaşık üçte ikisini oluşturan diyette yaygın olarak tüketilen içeceklerden biridir. Meyve sularının tıbbi ilaçlarla eş zamanlı tüketilmesi bitki-ilaç etkileşim riskini ortaya çıkarmaktadır. Önceki çalışmalarda C. paradisi, C. aurantifolia, C. maxima ve C. sinensis meyve sularının sitokrom (CYP)1A1 aktivitesini etkilediği belirtilmiştir. C. australasica, C. limon, C. reticulata × C. sinensis ve C. japonica meyve sularının CYP1A1 aktivitesi üzerindeki etkisini değerlendiren bir çalışma bulunmamaktadır. Gereç ve Yöntem: Bu çalışmada, narenciye meyve sularının sıçan karaciğer dokusu üzerindeki CYP1A1 aktivitesini 7-etoksirezorufin O-deetilaz yöntemi kullanarak değerlendirilmiştir. Sonuç ve Tartışma: Çalışılan altı meyve suyu arasında C. sinensis, C. japonica ve C. australasica'nın CYP1A1 aktivitesini sırasıyla %50, %50 ve %60 oranında inhibe ettiği bulunmuştur. Bu meyve suları, CYP 1A1 izoenzimi tarafından metabolize olan ilaçlar alınırken dikkatli kullanılmalıdır.

Anahtar Kelimeler

• Citrus
• CYP1A1
• EROD
• Meyve suyu

MODULATION OF CYP1A1 ACTIVITY IN RAT MICROSOMES BY CITRUS L. FRUIT JUICES

Öz

Objectives: Juices prepared from citrus fruits make up almost two-thirds of the worldwide market for juice, which makes them one of the most common beverages in the diet. The simultaneous consumption of fruit juices and medicinal drugs poses a risk of herb-drug interactions. Previous studies have noted that Citrus paradisi Macfad., Citrus aurantifolia Linn., Citrus maxima (Burm) Merrill, and Citrus sinensis (L.) Osbeck juices affect cytochrome (CYP) 1A1 activity. Studies testing the effects of Citrus australasica F. Muell., Citrus limon (L.) Osbeck, Citrus reticulata Blanco× Citrus sinensis (L.) Osbeck, and Citrus japonica Thunb. fruit juices on CYP1A1 activity are lacking. Material and Methods: This study evaluate the CYP1A1 activity of Citrus fruit juices on rat liver tissues using the 7-ethoxyresorufin O-deethylase (EROD) assay. Result and Discussion: Among the six fruit juices studied, C. sinensis, C. japonica and C. australasica were found to inhibit CYP1A1 activity by 50%, 50% and 60%, respectively. These juices should be used with caution when taking medicines metabolized by the CYP1A1 isoenzyme.

Anahtar Kelimeler

• Citrus
• CYP1A1
• EROD
• Fruit juice

Destekleyen Kurum

None

Etik Beyan

Before beginning the study, approval from the ethical committee was secured, as per a decision of the Ankara University Health Sciences Ethical Committee dated March 14, 2018, and numbered 2018-6-45.

Teşekkür

This study was presented as a poster presentation in the form of a summary at the 14th International Symposium on Pharmaceutical Sciences (ISOPS) Symposium.

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