A VALIDATED ADSORPTIVE STRIPPING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD FOR THE QUANTIFICATION OF LINAGLIPTIN FROM URINE SAMPLE

Öz

Objective: In this study, a low-cost, sensitive and practical electroanalytical method was developed and validated for the determination of Linagliptin (LNG), a therapeutic agent used in the treatment of Diabetes Mellitus, one of the most common chronic diseases worldwide, characterized by high blood glucose levels and directly affecting millions of people. Material and Method: Electroanalytical studies were completed using screen-printed carbon electrodes in pH: 8 Britton - Robinson buffer solution. Mechanistic studies were carried out by cyclic voltammetry technique. Adsorptive stripping differential pulse voltammetry technique was used for the electrochemical determination of LNG. Result and Discussion: The developed method exhibited linearity in the range of 0.1 - 7.5 µM and the limit of detection value was calculated as 37 nM. The developed method was applied for the determination of LNG from urine and a recovery value of 100.02% was obtained.

Anahtar Kelimeler

• Adsorptive stripping differential pulse voltammetry
• antidiabetic drug
• electrochemical determination
• linagliptin

İDRARDAN LİNAGLİPTİN TAYİNİ İÇİN ADSOPRTİF SIYIRMA DİFERANSİYEL PULS VOLTAMETRİK YÖNTEM GELİŞTİRİLMESİ VE VALİDASYONU

Öz

Amaç: Bu çalışmada, dünya çapında en yaygın kronik hastalıklardan biri olan, yüksek kan şekeri ile karakterize, ve milyonlarca insanı doğrudan etkileyen bir rahatsızlık olan Diabetes Mellitus tedavisinde kullanılan terapötik bir ajan olan Linagliptin (LNG) tayini için düşük maliyetli, hassas ve pratik bir elektroanalitik yöntem geliştirilmiş ve valide edilmiştir. Gereç ve Yöntem: Elektroanalitik çalışmalar pH: 8 Britton - Robinson tampon çözelti ortamında perde baskılı carbon elektrotlar kullanılarak tamamlanmıştır. Mekanistik çalışmalar dönüşümlü voltametri tekniği ile yapılmıştır. LNG'nin elektrokimyasal tayini için de adsorptif sıyırma diferansiyel puls voltametri tekniği kullanılmıştır. Sonuç ve Tartışma: Geliştirilen yöntem 0.1 – 7.5 µM aralığında doğrusallık sergilemiş ve gözlenebilme sınırı 37 nM olarak hesaplanmıştır. Geliştirilen yöntem idrardan LNG tayini için uygulanmış ve % 100.02'lik bir geri kazanım değeri elde edilmiştir.

Anahtar Kelimeler

• Adsorptive sıyırma diferansiyel puls voltametri
• antidiyabetik ilaç
• elektrokimyasal analiz
• linagliptin

Kaynakça

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