SÜLFAMETOKSAZOLÜN ENDOTEL VE FİBROBLAST HÜCRELERİ ÜZERİNDEKİ ETKİLERİNİN DEĞERLENDİRİLMESİ

Öz

Amaç: Sülfametoksazol (SMX), bakteriyel enfeksiyonların tedavisinde kullanılan bir sülfonamid türevi ilaçtır. SMX sadece enfeksiyon tedavisinde değil, aynı zamanda doku rejenerasyonu ve yara iyileşmesindeki olası rolüyle de potansiyel terapötik uygulama alanları ilgi uyandırmaktadır. Bu çalışmada SMX’in fibroblast ve endotel hücrelerinin canlılığı, migrasyonu ve kollajen gen ekspresyonu üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada, endotel hücre hattı olarak HUVEC ve fibroblast hücre hattı olarak ise L929 kullanıldı. Hücre canlılığı MTT testi ile, migrasyon inhibisyonu yara iyileşme testi ile ve COL1A1 gen ekspresyonu ise qRT-PCR yöntemi ile değerlendirildi. Sonuç ve Tartışma: SMX’in, endotel ve fibroblast hücrelerinde yara iyileşmesini destekleyecek bazı olumlu etkiler gösterdiği ancak bazı konsantrasyonların hücresel süreçleri olumsuz etkileyebileceği bulundu. SMX’in kullanıldığı terapötik alanlarının genişletilebilmesi için daha ayrıntılı in vitro araştırmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler

• Endotelyal hücre
• fibroblast hücresi
• sülfametoksazol
• yara iyileşmesi

EVALUATION OF THE EFFECTS OF SULFAMETHOXAZOLE ON ENDOTHELIAL AND FIBROBLAST CELLS

Öz

Objective: Sulfamethoxazole (SMX) is a sulfonamide derivative drug used in the treatment of bacterial infections. The potential role of SMX not only in the treatment of infections but also in tissue regeneration and wound healing has aroused interest in its potential therapeutic applications. In this study, we aimed to determine the effects of SMX on fibroblast and endothelial cell viability, migration and collagen gene expression. Material and Method: In this study, HUVEC was used as endothelial cell line and L929 as fibroblast cell line. Cell viability was evaluated by MTT assay, migration inhibition by wound healing assay and COL1A1 gene expression by qRT-PCR method. Result and Discussion: SMX was found to show some positive effects on endothelial and fibroblast cells to promote wound healing, but some concentrations may negatively affect cellular processes. More detailed in vitro research is needed to expand the therapeutic areas in which SMX is used.

Anahtar Kelimeler

• Endothelial cell
• fibroblast cell
• sulfamethoxazole
• wound healing

Kaynakça

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